Novel conserved oligodendrocyte surface epitope identified by monoclonal antibody 4860

Abstract

Oligodendrocytes are the myelinating cells of the central nervous system. They differentiate from oligodendrocyte precursor cells through several intermediate states that can be followed by characteristic morphological changes and the expression of marker molecules. However, most oligodendrocyte lineage markers demarcate either the precursor or the differentiated oligodendrocyte in restricted subcellular compartments. Here, we describe a novel marker of the oligodendrocyte lineage recognised by the monoclonal antibody clone 4860. It selectively labels the surfaces of differentiated oligodendrocytes in culture and clearly differs from other oligodendrocyte markers. Importantly, the 4860 epitope highlights developing white matter tracts in rodent and avian brains and thus represents a useful and conserved feature. The 4860 epitope is not associated with protein backbones as revealed by the related 487/L5 antibody. Furthermore, the epitope disappears upon lipid extraction from cryosections or inhibition of sphingolipid synthesis in cultured oligodendrocytes. Thus, we conclude that mAb 4860 represents a novel lipid-based oligodendrocyte marker.

Regulatory Mechanisms that Mediate Tenascin C-Dependent Inhibition of Oligodendrocyte Precursor Differentiation

“…Immunocytochemical stainings were performed as previously described (von Holst et al, 2006;Czopka et al, 2009a).…”

Laser Lesion in the Mouse Visual Cortex Induces a Stem Cell Niche-Like Extracellular Matrix, Produced by Immature Astrocytes

“…The 4860 epitope was found on astroglial cells, shown by double staining with GFAP (Figure 8). In the healthy CNS, the epitope is part of glycolipids on cells of the oligodendrocyte lineage that have nearly completed differentiation and have already downregulated NG2 and other earlier markers (Czopka et al, 2009). During postnatal development, the epitope is strongly expressed and shows a diffuse staining pattern in the adult cortex.…”

“…Here, both antibodies have been shown to label different subpopulations of neural stem/progenitor cells. A related epitope, detected by the mAb 4860, has been found on cells of the oligodendrocyte lineage in the developing CNS (Czopka et al, 2009). Perineuronal nets (PNNs) are a specialized form of matrix that enwraps subtypes of neurons and is associated with plasticity restriction in the adult CNS.…”

Pluripotent stem cell-derived radial glia-like cells as stable intermediate for efficient generation of human oligodendrocytes

“…F). This antigen was recently shown to be expressed in rodent oligodendrocytes between the O4‐ and MBP‐positive stage (Czopka et al, ). The expression of MBP confirms the capacity for myelin gene expression (Fig.…”

“…Differentiation along the oligodendrocyte lineage was clearly demonstrated by the expression of several lineage‐specific markers, such as NG2, O4, 4860, CXCR7, MBP, MAG, MOG, MAL1/2, and MYT1 (Buchet and Baron‐Van Evercooren, ; Czopka et al, ; Frank ; Gottle et al, ; Nielsen et al, ). In addition, RT‐PCR analysis at distinct stages during oligodendroglial in vitro differentiation revealed the sequential activation of the oligodendrocyte‐specific transcription factors NKX6.2 , OLIG1/2 , NKX2.2 , and SOX10 (Rowitch, ; Wegner, ).…”