Novel curcumin-loaded gel-core hyaluosomes with promising burn-wound healing potential: Development, in-vitro appraisal and in-vivo studies

El-Refaie, Wessam M.; Elnaggar, Yosra S.R.; El-Massik, Magda A.; Abdallah, Ossama Y.

doi:10.1016/j.ijpharm.2015.03.052

Cited by 120 publications

(83 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The bags were transferred to ambered glass bottles containing 10 ml of water:ethanol (1:1), in horizontal shaking (Horizontal shaking water bath Wisebath Õ , DAIHAN-Scientific Co., Ltd., Seoul, Korea) water bath at 100 strokes/min, adjusted to 37 ± 0.5 C. The total volume was withdrawn at different time intervals (0.5, 1, 2, 3, 4 and 5 h) and replaced with an equivalent volume of a previously warmed fresh medium. The amount of Cur released was measured using UV spectrophotometer at lambda max 431 nm (El-Refaie et al, 2015). The percentage release was calculated on the cumulative drug released at different time intervals.…”

Section: In Vitro Release Testing (Dialysis Method)mentioning

confidence: 99%

“…Samples were measured spectrophotometrically. The mucosa was then gently washed to remove excess formula, and entrapped Cur was extracted by soaking in acetone overnight (El-Refaie et al, 2015). The samples were centrifuged (Cooling centrifuge, Centurion Scientific Ltd, UK) at 4000 rpm and 4 C for 30 min; samples were appropriately diluted and analyzed spectrophotometrically.…”

Section: Ex Vivo Permeation and Retention Testmentioning

confidence: 99%

See 1 more Smart Citation

A new approach for treatment of precancerous lesions with curcumin solid–lipid nanoparticle-loaded gels: in vitro and clinical evaluation

et al. 2015

Self Cite

View full text Add to dashboard Cite

Objective: Preparation and characterization of curcumin solid-lipid nanoparticle (CurSLN)-loaded mucoadhesive gel for local treatment of oral precancerous lesions with low dose. Methodology: The formulated CurSLNs were dispersed in a mucoadhesive gel matrix to be applied to the buccal mucosa. Conventional mucoadhesive gel using binary system was adopted. The prepared gels were evaluated for in vitro drug dialysis, ex vivo mucoadhesion test and ex vivo permeation study using chicken buccal mucosa. Short-term clinical evaluation was carried out on 10 patients suffering oral erythroplakia in terms of pain index and lesion size measurement.1 Results:The results showed that the loaded gel with CurSLN showed good mucoadhesion property and 25 min in vivo residence time. In addition to stability enhancement for the Cur powder. All formulae did not show any drug permeated, however, significant amount of Cur was retained within the chicken buccal mucosal tissue confirmed by histological examination. Significant reduction in pain, and complete healing was observed after 6 weeks of treatment. Conclusion:The local use of Cur in low dose is a promising option for treatment of precancerous lesions. The lack of local anti-inflammatory compounds with reduced side effects intensifies the importance of studying natural products for this purpose.

show abstract

Section: In Vitro Release Testing (Dialysis Method)mentioning

confidence: 99%

Section: Ex Vivo Permeation and Retention Testmentioning

confidence: 99%

A new approach for treatment of precancerous lesions with curcumin solid–lipid nanoparticle-loaded gels: in vitro and clinical evaluation

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…6,[14][15][16][17][18][19][20][21][22] Lipidbased liquid crystalline nanoparticles (LCN) have recently captured the obvious interest of pharmaceutical research to enhance the bioavailability of lipophilic drugs. 23 LCN enjoy characteristics of biocompatibility, bioadhesivity, and biodegradability offering various advantages for brain delivery.…”

Section: Introductionmentioning

confidence: 99%

Novel piperine-loaded Tween-integrated monoolein cubosomes as brain-targeted oral nanomedicine in Alzheimer’s disease: pharmaceutical, biological, and toxicological studies

Elnaggar¹,

Etman²,

Abdelmonsif³

et al. 2015

IJN

141

View full text Add to dashboard Cite

Alzheimer’s disease (AD) is one of the most patient devastating central nervous system diseases with no curative therapy. An effective oral therapy with brain-targeting potential is required that is hampered by blood–brain barrier. Piperine (PIP) is a natural alkaloid with memory enhancing potentials. Oral PIP delivery suffers from its hydrophobicity and first-pass metabolism. In this study, novel Tween-modified monoolein cubosomes (T-cubs) were elaborated as bioactive nanocarriers for brain-targeted oral delivery of PIP. Seven liquid crystalline nanoparticles (cubosomes) were prepared testing different bioactive surfactants (Tween 80, poloxamer, and Cremophor). Full in vitro characterization was carried out based on particle size, zeta potential, polydispersity index, entrapment efficiency, and in vitro release. Morphological examination and structure elucidation were performed using transmission and polarizing microscopes. Sporadic dementia of Alzheimer’s type was induced in 42 male Wistar rats on which full behavioral and biochemical testing was conducted. Brain toxicity was assessed based on Caspase-3 assay for apoptosis and tumor necrosis factor-α for inflammation. Liver and kidney toxicity studies were conducted as well. Among others, T-cubs exhibited optimum particle size (167.00±10.49 nm), polydispersity index (0.18±0.01), and zeta potential (−34.60±0.47 mv) with high entrapment efficiency (86.67%±0.62%). Cubs could significantly sustain PIP in vitro release. In vivo studies revealed T-cubs potential to significantly enhance PIP cognitive effect and even restore cognitive function to the normal level. Superiority of T-cubs over others suggested brain-targeting effect of Tween. Toxicological studies contended safety of cubs on kidney, liver, and even brain. T-cubs exhibited potential anti-inflammatory and anti-apoptotic activity of loaded PIP, indicating potential to stop AD progression that was first suggested in this article. Novel oral nanoparticles elaborated possess promising in vitro and in vivo characteristics with high safety for effective chronic treatment of AD.

show abstract

“…26,48 The receptor compartment of the Franz diffusion cells was filled with 9 mL of SPB of pH 5.5 5,41 which was suitable for a proper sink condition. The temperature was maintained at 32°C±0.5°C 8,45,49 with shaking at 100 rpm throughout the experiment.…”

Section: Permeation Studiesmentioning

confidence: 99%

Novel lecithin-integrated liquid crystalline nanogels for enhanced cutaneous targeting of terconazole: development, in vitro and in vivo studies

Elnaggar

Talaat

Bahey-El-Din

et al. 2016

IJN

View full text Add to dashboard Cite

Novel curcumin-loaded gel-core hyaluosomes with promising burn-wound healing potential: Development, in-vitro appraisal and in-vivo studies

Cited by 120 publications

References 54 publications

A new approach for treatment of precancerous lesions with curcumin solid–lipid nanoparticle-loaded gels: in vitro and clinical evaluation

A new approach for treatment of precancerous lesions with curcumin solid–lipid nanoparticle-loaded gels: in vitro and clinical evaluation

Novel piperine-loaded Tween-integrated monoolein cubosomes as brain-targeted oral nanomedicine in Alzheimer’s disease: pharmaceutical, biological, and toxicological studies

Novel lecithin-integrated liquid crystalline nanogels for enhanced cutaneous targeting of terconazole: development, in vitro and in vivo studies

Contact Info

Product

Resources

About

Novel curcumin-loaded gel-core hyaluosomes with promising burn-wound healing potential: Development, in-vitro appraisal and in-vivo studies

Cited by 120 publications

References 54 publications

A new approach for treatment of precancerous lesions with curcumin solid–lipid nanoparticle-loaded gels: in vitro and clinical evaluation

A new approach for treatment of precancerous lesions with curcumin solid–lipid nanoparticle-loaded gels: in vitro and clinical evaluation

Novel piperine-loaded Tween-integrated monoolein cubosomes as brain-targeted oral nanomedicine in Alzheimer&rsquo;s disease: pharmaceutical, biological, and&nbsp;toxicological studies

Novel lecithin-integrated liquid crystalline nanogels for enhanced cutaneous targeting of terconazole: development, in vitro and in vivo studies

Contact Info

Product

Resources

About

Novel piperine-loaded Tween-integrated monoolein cubosomes as brain-targeted oral nanomedicine in Alzheimer’s disease: pharmaceutical, biological, and toxicological studies