Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities

Abstract

The mechanical properties and anti-caries effect of a novel anti-caries adhesive containing poly (amidoamine) dendrimer (PAMAM) and dimethylaminododecyl methacrylate (DMADDM) were investigated for the first time. Microtensile bond strength and surface charge density were measured for the novel anti-caries adhesives. Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii were chosen to form three-species biofilms. Lactic acid assay, MTT (3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, exopolysaccharide staining and live/dead staining were performed to study anti-biofilm effect of the adhesive. The TaqMan realtime polymerase chain reaction was used to study the proportion change in three-species biofilms of different groups. The Scanning Electron Microscope (SEM) was used to observe the remineralization effect of PAMAM and DMADDM. The results showed that incorporating PAMAM and DMADDM into adhesive had no adverse effect on the dentin bond strength. The 1% PAMAM and 5% DMADDM adhesive group showed anti-biofilm properties and developed a healthier biofilm with a lower chance of inducing dental caries. Combination of 1% PAMAM and 5% DMADDM solution maintained remineralization capability on dentin, similar to that using 1% PAMAM alone. In conclusion, the adhesive containing PAMAM and DMADDM had strong antimicrobial properties and biological remineralization capabilities, and is promising for anti-caries clinical applications.

The Antibacterial Effects of Quaternary Ammonium Salts in the Simulated Presence of Inhibitors in Root Canals: A Preliminary In-Vitro Study

“…MIC and MBC of DMADDM and DMAHDM against mixed suspension of four bacteria were identified by broth microdilution, as described previously [11]. The starting concentrations for DMADDM and DMAHDM and CHX were 200 and 100 µg/mL, respectively.…”

“…After the MIC determination, aliquots of 10 µL bacterial suspensions from the wells with no visible cells, where bacterial growth was inhibited, was seeded in BHI agar plates and incubated under anaerobic conditions for 48 h at 37 • C with 5% CO 2 . After calculating the colony forming units, the MBC values were determined [11].…”

“…Quaternary ammonium compounds (QAMs) can be co-polymerized and incorporated to dental materials, such as resin composites, adhesives, intracanal medicaments, root canal sealers or root canal irrigants [9][10][11][12][13]. Thus, they can provide long-term contact-inhibition against bacteria [9].…”

“…This unique antibacterial mechanism made QAMs effective against a wide range of microbes and also minimized the possibility of the development of resistance by microbes. Two recently developed QAM compounds, dimethylaminododecyl methacrylate (DMADDM) and dimethylaminohexadecyl methacrylate (DMAHDM), were proven to be more effective than conventional QAMs [11][12][13][14][15][16][17]. The resistance against aging is an added benefit of these novel compounds which makes them remain active for a longer duration and inhibit bacterial colonization.…”

Effect of Novel Micro-Arc Oxidation Implant Material on Preventing Peri-Implantitis

“…The biofilm was rinsed with PBS before being drained of water and stained using the BacLight live/dead bacterial viability kit (Molecular Probes, Eugene, OR, USA) for 15 min in the dark [31]. Live bacteria cells emitted green fluorescence, while dead cells were stained with propidium iodide and emitted red fluorescence.…”