Novel detergent for whole organ tissue engineering

Abstract

Whole organ tissue engineering for various organs, including the heart, lung, liver, and kidney, has demonstrated promising results for end-stage organ failure. However, the sodium dodecyl sulfate (SDS)-based protocol for standard decellularization has drawbacks such as clot formation in vascularized transplantation and poor cell engraftment in recellularization procedures. Preservation of the surface milieu of extracellular matrices (ECMs) might be crucial for organ generation based on decellularization/recellularization engineering. We examined a novel detergent, sodium lauryl ether sulfate (SLES), to determine whether it could overcome the drawbacks associated with SDS using rat heart and kidney. Both organs were perfused in an antegrade fashion with either SLES or SDS. Although immunohistochemistry for collagen I, IV, laminin, and fibronectin showed similar preservation in both detergents, morphological analysis using scanning electron microscopy and an assay of glycosaminoglycan content on ECMs showed that SLES-treated tissues had better-preserved ECMs than SDS-treated tissues. Mesenteric transplantation revealed SLES did not induce significant inflammation, as opposed to SDS. Platelet adhesion to decellularized tissues was significantly reduced with SLES. Overall, SLES could replace older detergents such as SDS in the decellularization process for generation of transplantable recellularized organs.

Sodium hydroxide based non-detergent decellularizing solution for rat lung

“…In a previous report, 50 mg/L (0.005%) of residual detergent did not cause any adverse effects on recellularization. 24 However, there is the possibility that accidental insufficient rinsing might cause excessive levels of residual detergents in the scaffold. Because recellularization is similarly achieved after NaOH based decellularization as other detergent based decellularization, overall, an NaOH based solution may be superior in regard to both cost and safety among the studied decellularization solutions.…”

Development of xenogeneic decellularized biotubes for off‐the‐shelf applications

“…Tubular connective tissues were connected to a perfusion pump (TP‐10SA; AS ONE) and decellularized by the following procedure in a desterilized beaker in a heated thermostat bath. Tubular connective tissues were perfused with 1% sodium dodecyl sulfate (Nacalai Tesque, Inc, Kyoto, Japan) for 12 h, deionized water for 15 min, 1% Triton X‐100 (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) for 30 min, and 500 U/mL DNase (Worthington Biochemical Corp., Lakewood, NJ, USA) in phosphate‐buffered saline (Takara Bio Inc, Shiga, Japan) for 24 h, with each perfusion followed by rinses with deionized water.…”

Human breast cancer decellularized scaffolds promote epithelial‐to‐mesenchymal transitions and stemness of breast cancer cells in vitro

“…After briefly washing in Dulbecco's phosphate‐buffered saline (DPBS) solution (14190250; Invitrogen, China), the biopsies were kept in 50 ml DPBS solution and transferred to the lad at 4℃ within 4 hr. The tissues were cut into 1 mm 3 followed by blood cells removal with DPBS solution and transferred into 1% or 0.5% SLES solution (68585‐34‐2; Hebei Wanye Ltd., China) with gentle shaking at 30 rpm/min for control times (6, 12, and 18 hr) at room temperature (Kawasaki et al, ; Uygun et al, ). After SLES treatments, the tissues were washed extensively with sterilized water and DPBS solution with a gentle shaking for 8 × 2 hr at 50 rpm/min at room temperature to remove the residual SLES solution, respectively.…”