Novel distribution of cluster of differentiation 200 adhesion molecule in glial cells of the peripheral nervous system of rats and its modulation after nerve injury

Chang, Chiu-Yun; Lee, Yi-Hsuan; Jiang-Shieh, Ya-Fen; Chien, Hsiung–Fei; Pai, Man Hui; Chen, H. M.; Fong, Tsorng Harn; Wu, Ching Hsiang

doi:10.1016/j.neuroscience.2011.03.049

Cited by 13 publications

(9 citation statements)

References 59 publications

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“…Macrophages are recruited to the nerve injured site to eliminate fragmented myelin debris and dead cells (Dubovy, 2011 ). Cd200 is expressed in node of Ranvier and Schmidt-Lanterman incisures and is down-regulated following nerve injury (Chang et al, 2011 ). It has been suggested that Cd200 down-regulation might facilitate macrophage infiltration through the node of Ranvier to eliminate degenerated axons and myelin debris (Chang et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

“…Cd200 is expressed in node of Ranvier and Schmidt-Lanterman incisures and is down-regulated following nerve injury (Chang et al, 2011 ). It has been suggested that Cd200 down-regulation might facilitate macrophage infiltration through the node of Ranvier to eliminate degenerated axons and myelin debris (Chang et al, 2011 ). Also Synuclein α (Snca) is localised at nodes of Ranvier and Schmidt-Lanterman incisures, is up-regulated during myelination and down-regulated after nerve injury (D’Antonio et al, 2006 ) and might be involved in macrophage infiltration.…”

Section: Discussionmentioning

confidence: 99%

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Soluble Neuregulin1 Down-Regulates Myelination Genes in Schwann Cells

Soury

Fornasari

Morano

et al. 2018

Front. Mol. Neurosci.

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Peripheral nerves are characterised by the ability to regenerate after injury. Schwann cell activity is fundamental for all steps of peripheral nerve regeneration: immediately after injury they de-differentiate, remove myelin debris, proliferate and repopulate the injured nerve. Soluble Neuregulin1 (NRG1) is a growth factor that is strongly up-regulated and released by Schwann cells immediately after nerve injury. To identify the genes regulated in Schwann cells by soluble NRG1, we performed deep RNA sequencing to generate a transcriptome database and identify all the genes regulated following 6 h stimulation of primary adult rat Schwann cells with soluble recombinant NRG1. Interestingly, the gene ontology analysis of the transcriptome reveals that NRG1 regulates genes belonging to categories that are regulated in the peripheral nerve immediately after an injury. In particular, NRG1 strongly inhibits the expression of genes involved in myelination and in glial cell differentiation, suggesting that NRG1 might be involved in the de-differentiation (or “trans-differentiation”) process of Schwann cells from a myelinating to a repair phenotype. Moreover, NRG1 inhibits genes involved in the apoptotic process, and up-regulates genes positively regulating the ribosomal RNA processing, thus suggesting that NRG1 might promote cell survival and stimulate new protein expression. This in vitro transcriptome analysis demonstrates that in Schwann cells NRG1 drives the expression of several genes which partially overlap with genes regulated in vivo after peripheral nerve injury, underlying the pivotal role of NRG1 in the first steps of the nerve regeneration process.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Soluble Neuregulin1 Down-Regulates Myelination Genes in Schwann Cells

Soury

Fornasari

Morano

et al. 2018

Front. Mol. Neurosci.

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“…The normal presence of the ligands in Schwann cells and oligodendrocytes point to supplementary roles in addition to the phosphatidylserine “eat me” signal or the ceramide-induced signaling previously described. Other authors have shown that CD200, the ligand for the inhibitory immune receptor CD200R, is expressed in Schwann cells in the intact nerve [ 59 ]. In the CNS, this receptor induces a tonic anti-inflammatory signal contributing to set the threshold and magnitude of proinflammatory signaling [ 24 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

“…Whether the ligand of CD300f expressed on Schwann cells and oligodendrocytes also contributes to the maintenance of this tonic anti-inflammatory state is an open question. Chang and co-workers showed that CD200 is downregulated after crush injury in the site of lesion [ 59 ]. They hypothesized that, after nerve injury, CD200 is downregulated in order to decrease immunosuppression and enhance influx of macrophages and the inflammatory response to eliminate myelin and axonal debris.…”

Section: Discussionmentioning

confidence: 99%

CD300f immunoreceptor contributes to peripheral nerve regeneration by the modulation of macrophage inflammatory phenotype

Peluffo

Solari-Saquieres

Negro-Demontel

et al. 2015

J Neuroinflammation

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BackgroundIt has recently become evident that activating/inhibitory cell surface immune receptors play a critical role in regulating immune and inflammatory processes in the central nervous system (CNS). The immunoreceptor CD300f expressed on monocytes, neutrophils, and mast cells modulates inflammation, phagocytosis, and outcome in models of autoimmune demyelination, allergy, and systemic lupus erythematosus. On the other hand, a finely regulated inflammatory response is essential to induce regeneration after injury to peripheral nerves since hematogenous macrophages, together with resident macrophages and de-differentiated Schwann cells, phagocyte distal axonal and myelin debris in a well-orchestrated inflammatory response. The possible roles and expression of CD300f and its ligands have not been reported under these conditions.MethodsBy using quantitative PCR (QPCR) and CD300f-IgG2a fusion protein, we show the expression of CD300f and its ligands in the normal and crush injured sciatic nerve. The putative role of CD300f in peripheral nerve regeneration was analyzed by blocking receptor-ligand interaction with the same CD300f-IgG2a soluble receptor fusion protein in sciatic nerves of Thy1-YFP-H mice injected at the time of injury. Macrophage M1/M2 polarization phenotype was also analyzed by CD206 and iNOS expression.ResultsWe found an upregulation of CD300f mRNA and protein expression after injury. Moreover, the ligands are present in restricted membrane patches of Schwann cells, which remain stable after the lesion. The lesioned sciatic nerves of Thy1-YFP-H mice injected with a single dose of CD300f-IgG2a show long lasting effects on nerve regeneration characterized by a lower number of YFP-positive fibres growing into the tibial nerve after 10 days post lesion (dpl) and a delayed functional recovery when compared to PBS- or IgG2a-administered control groups. Animals treated with CD300f-IgG2a show at 10 dpl higher numbers of macrophages and CD206-positive cells and lower levels of iNOS expression than both control groups. At later time points (28 dpl), increased numbers of macrophages and iNOS expression occur.ConclusionsTaken together, these results show that the pair CD300f ligand is implicated in Wallerian degeneration and nerve regeneration by modulating both the influx and phenotype of macrophages.

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“…In addition, nerve injury is associated with microglial activation and it has been reported that facial nerve transaction induced a greater degree of microglial activation in CD200-deficient, compared with wildtype, mice . Similarly the neurodegenerative changes that occurred following sciatic nerve crush was associated with a profound loss of CD200 and evidence of macrophage activation (Chang et al, 2011).…”

Section: The Importance Of the Interaction Between Cd200 And Cd200r Imentioning

confidence: 93%