Novel DNA nanoflower biosensing technologies towards next-generation molecular diagnostics

Sheng, Jifang; Pi, Yan; Wang, Binpan; Chen, Ming; Chang, Kai

doi:10.1016/j.tibtech.2022.08.011

Cited by 17 publications

(6 citation statements)

References 83 publications

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“…Based on Watson-Crick base-pairing, single-stranded DNA/RNA can be designed to self-assemble into three-dimensional structures in different shapes and sizes. A unique advantage of DNA/RNA nanomaterials is that the rich information embedded in DNA/RNA sequences can be utilized to include aptamer sequences for cell-type specific targeting and anti-sense sequences for biosensing ( 54 ). Thus, the release of the cargo in DNA/RNA nanoparticles can be elaborately controlled by specific cell types and selective cellular states (e.g., the expression of certain genes) ( 55 ).…”

Section: Targeted Therapeutic Delivery Via Functionalized Nanoparticlesmentioning

confidence: 99%

A bacterial enzyme may correct 2-HG accumulation in human cancers

Yin

2023

Front. Oncol.

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A significant proportion of lower-grade glioma as well as many other types of human cancers are associated with neomorphic mutations in IDH1/2 genes (mIDH1/2). These mutations lead to an aberrant accumulation of 2-hydroxyglutarate (2-HG). Interestingly, even cancers without mIDH1/2 can exhibit increased levels of 2-HG due to factors like hypoxia and extracellular acidity. Mounting evidence demonstrates that 2-HG competitively inhibits α-ketoglutarate dependent enzymes, such as JmjC-domain-containing histone demethylases (JHDMs), ten-eleven translocation enzymes (TETs), and various dioxygenases (e.g., RNA m6A demethylases and prolyl hydroxylases). Consequently, the hypermethylation of DNA, RNA, and histones, and the abnormal activities of hypoxia-inducible factors (HIFs) have profound impacts on the establishment of cancer metabolism and microenvironment, which promote tumor progression. This connection between the oncometabolite 2-HG and glioma holds crucial implications for treatments targeting this disease. Here, I hypothesize that an ectopic introduction of a bacterial 2-hydroxyglutarate synthase (2-HG synthase) enzyme into cancer cells with 2-HG accumulation could serve as a promising enzyme therapy for glioma and other types of cancers. While absent in human metabolism, 2-HG synthase in bacterial species catalyzes the conversion of 2-HG into propionyl-CoA and glyoxylate, two metabolites that potentially possess anti-tumor effects. For a broad spectrum of human cancers with 2-HG accumulation, 2-HG synthase-based enzyme therapy holds the potential to not only correct 2-HG induced cancer metabolism but also transform an oncometabolite into metabolic challenges within cancer cells.

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Section: Targeted Therapeutic Delivery Via Functionalized Nanoparticlesmentioning

confidence: 99%

A bacterial enzyme may correct 2-HG accumulation in human cancers

Yin

2023

Front. Oncol.

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“…Therefore, scanning electron microscopy (SEM) was employed next to observe the microstructure of the three types of prepared DNA hydrogels. Although all three exhibited a characteristic nanoflower microstructure (Figure 4A-C) comprised of RCA-generated ssDNA and had similar diameters (~500 nm) [8], the isolated nanoflower microstructures tended to connect with each other (yellow arrows, Figure 4B,C) with an increased degree of hydrogen bonding, gradually forming a porous sheet structure (red arrows, Figure 4C). This structure was similar to the microstructure of a DNA hydrogel produced with the hybridization chain reaction (HCR) technique [45,46], which can yield double helices [47].…”

Section: Characterization Of Mechanical Properties and Microstructure...mentioning

confidence: 99%

“…Deoxyribonucleic acid (DNA) hydrogels are a promising class of macroscopic threedimensional (3D) materials that offer a unique combination of high hydrophilicity and mechanical properties in polymer hydrogels [1] along with the remarkable biological functions of DNA such as structural designability, biocompatibility, selection specificity, molecular recognition ability, and responsiveness to environmental factors [2,3]. Because of these advantages, DNA hydrogels have wide-ranging applications in diverse fields such as food safety [4,5], medical diagnostics [6][7][8], environmental analysis [9,10], and controllable drug delivery and release [11,12]. Despite the availability of different chemical covalent linkages [13,14] and non-covalent physical cross-linking methods for preparing DNA hydrogels [15,16], the substantial cost of bulk-scale fabrication of pure DNA hydrogels and their low mechanical properties significantly limit their potential applications [1,17].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Impact of Hydrogen Bonding Degree in Long Single-Stranded DNA (ssDNA) Generated with Dual Rolling Circle Amplification (RCA) on the Preparation and Performance of DNA Hydrogels

Wang

Zhang

et al. 2023

Biosensors

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DNA hydrogels have gained significant attention in recent years as one of the most promising functional polymer materials. To broaden their applications, it is critical to develop efficient methods for the preparation of bulk-scale DNA hydrogels with adjustable mechanical properties. Herein, we introduce a straightforward and efficient molecular design approach to producing physically pure DNA hydrogel and controlling its mechanical properties by adjusting the degree of hydrogen bonding in ultralong single-stranded DNA (ssDNA) precursors, which were generated using a dual rolling circle amplification (RCA)-based strategy. The effect of hydrogen bonding degree on the performance of DNA hydrogels was thoroughly investigated by analyzing the preparation process, morphology, rheology, microstructure, and entrapment efficiency of the hydrogels for Au nanoparticles (AuNPs)–BSA. Our results demonstrate that DNA hydrogels can be formed at 25 °C with simple vortex mixing in less than 10 s. The experimental results also indicate that a higher degree of hydrogen bonding in the precursor DNA resulted in stronger internal interaction forces, a more complex internal network of the hydrogel, a denser hydrogel, improved mechanical properties, and enhanced entrapment efficiency. This study intuitively demonstrates the effect of hydrogen bonding on the preparation and properties of DNA hydrogels. The method and results presented in this study are of great significance for improving the synthesis efficiency and economy of DNA hydrogels, enhancing and adjusting the overall quality and performance of the hydrogel, and expanding the application field of DNA hydrogels.

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“…28 In addition, multi-modal signal detection shows greater flexibility in dealing with complex biological samples through stability enhancement by complementing different detection modes. 29 Although some excellent review papers have discussed single-modal composite probes or multi-modal sensing technologies, [30][31][32][33][34][35][36][37][38][39] to our knowledge, there is a lack of review on multi-modal composite nanoprobes and related sensing applications in the literature.…”

Section: Introductionmentioning

confidence: 99%