Novel Dolabellane-Type Diterpene Alkaloids with Lipid Metabolism Promoting Activities from the Seeds of Nigella sativa

Abstract

[structure: see text] Four new dolabellane-type diterpene alkaloids, nigellamines A(1) (1), A(2) (2), B(1) (3), and B(2) (4), were isolated from the seeds of Nigella sativa. Their absolute stereostructures were determined on the basis of chemical and physicochemical evidence. Nigellamines A(1) (1), B(1) (3), and B(2) (4) were found to show potent lipid metabolism promoting activity in primary cultured mouse hepatocytes, and their activities were equivalent to that of a PPAR-alpha agonist, clofibrate.

A review on therapeutic potential of Nigella sativa: A miracle herb

“…The effects of the above-mentioned drugs on plasma nitrite, a stable metabolite of nitric oxide and brain GABA content were also studied. TQ (10 and 20 mg/kg) produced significant antianxiety effects in unstressed mice without altering nitrite levels, but only the higher dose (20 mg/kg) of TQ increased the GABA content in unstressed mice. In stressed mice, TQ (20 mg/kg) showed anxiolytic effects, with a significant decrease in plasma nitrite and reversal of the decreased brain GABA content.…”

“…TQ (10 and 20 mg/kg) produced significant antianxiety effects in unstressed mice without altering nitrite levels, but only the higher dose (20 mg/kg) of TQ increased the GABA content in unstressed mice. In stressed mice, TQ (20 mg/kg) showed anxiolytic effects, with a significant decrease in plasma nitrite and reversal of the decreased brain GABA content. Pre-treatment with methylene blue enhanced the antianxiety effect of TQ in both unstressed and stressed mice.…”

“…Examples of various other reported chemical components includes nigellone,avenasterol-5-ene, avenasterol-7 -ene, campesterol, cholesterol, citrostadienol, cycloeucalenol, ,gramisterol, lophenol, obtusifoliol, stigmastanol, stigmasterol-7-ene, β-amyrin, butyrospermol, cycloartenol, 24- gluco-pyranosyl] hederagenin, volatile oil (0.5-1.6%), fatty oil (35.6-41.6%), oleic acid, esters of unsaturated fatty acids with C15 and higher terpenoids, esters of dehydrostearic and linoleic acid, aliphatic alcohol, β-unsaturated hydroxy ketone, hederagenin glycoside, melanthin, melanthigenin, bitter principle, tannin, resin, protein, reducing sugar, glycosidal saponin, 3 23-dihydroxy-28-methy-lolean-12-enoate,stigma-5, 22-dien-3-β-D-gluco-pyranoside, cycloart-23-methyl-7, 20, 22-triene-3β, 25-diol, nigellidine-4-O-sulfite, N. mines A3, A4, A5, C, N. mines A1, A2, B1, and B2 [18][19][20][21][22] .…”

“…Animals were injected with vehicle, TQ (10,20 mg/kg), omeprazole (10,20 mg/kg) or their combination (10 mg/kg). Thirty minutes later, pyloric ligation was carried out and followed consequently with ischemia for another 30 min, abided by reperfusion for 120 min.…”

“…Male Wistar rats were subjected to I/R and were injected with either NO (2.5 and 5.0 mL/kg, p.o.) or TQ (5,20,50 and 100 mg/kg, p.o.). The results showed that I/R elevated the levels of lipid peroxide and lactate dehydrogenase, while decreased those of reduced GSH and SOD.…”