Novel Effects of Minocycline on Ca2+-Dependent Cl− Secretion in Human Airway Epithelial Calu-3 Cells

Ito, Yasushi; Son, Masami; Kumakura, Hiroaki; Yamaki, Kenichi

doi:10.1006/taap.2001.9261

Cited by 13 publications

(8 citation statements)

References 41 publications

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“…Moon et al (1997) has reported that the basolateral membrane in Calu-3 contains K Ca channels, but no K cAMP channel, because it responds to thapsigargin but not to forskolin. In contrast, our previous data (Ito et al, 2001b) and those of Devor et al (1999) have shown that forskolin generated sustained I sc . These forskolin-induced responses are insensitive to charybdotoxin (an hIK1 blocker) and BAPTA-AM (an intracellular Ca 2ϩ chelator) in the Calu-3 cell monolayer (Ito et al, 2001b).…”

Section: Discussioncontrasting

confidence: 78%

Bisphenol A Inhibits Cl⁻ Secretion by Inhibition of Basolateral K⁺ Conductance in Human Airway Epithelial Cells

Ito

Sato

Son

et al. 2002

J Pharmacol Exp Ther

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Section: Discussioncontrasting

confidence: 78%

Bisphenol A Inhibits Cl⁻ Secretion by Inhibition of Basolateral K⁺ Conductance in Human Airway Epithelial Cells

Ito

Sato

Son

et al. 2002

J Pharmacol Exp Ther

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“…DIDS blocks many different Cl Ϫ channels and is reported to inhibit basolateral Cl Ϫ conductance in Calu-3 epithelia (17,37). To study Cl Ϫ current across the basolateral membrane, we first blocked electrogenic transepithelial ion transport by treating the cells with amiloride and bumetanide.…”

Section: A Basolateral CLmentioning

confidence: 99%

“…Willumsen et al (41) reported a small basolateral Cl Ϫ conductance in human airway epithelia. Other studies reported the presence of an outwardly rectifying basolateral Cl Ϫ channel in the submucosal gland cell line Calu-3 (17,37,42), where the channel was regulated by cAMP agonists. In bovine airway epithelia, basolateral Cl Ϫ conductance was attributed to an inwardly rectifying Cl Ϫ channel that was regulated by agents that elevate cAMP levels (39).…”

mentioning

confidence: 95%

Basolateral chloride current in human airway epithelia

Itani¹,

Lamb²,

Melvin³

et al. 2007

American Journal of Physiology-Lung Cellular and Molecular Phys

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Electrolyte transport by airway epithelia regulates the quantity and composition of liquid covering the airways. Previous data indicate that airway epithelia can absorb NaCl. At the apical membrane, cystic fibrosis transmembrane conductance regulator (CFTR) provides a pathway for Cl− absorption. However, the pathways for basolateral Cl− exit are not well understood. Earlier studies, predominantly in cell lines, have reported that the basolateral membrane contains a Cl− conductance. However, the properties have varied substantially in different epithelia. To better understand the basolateral Cl− conductance in airway epithelia, we studied primary cultures of well-differentiated human airway epithelia. The basolateral membrane contained a Cl− current that was inhibited by 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS). The current-voltage relationship was nearly linear, and the halide selectivity was Cl− > Br− >> I−. Several signaling pathways increased the current, including elevation of cellular levels of cAMP, activation of protein kinase C (PKC), and reduction of pH. In contrast, increasing cell Ca2+ and inducing cell swelling had no effect. The basolateral Cl− current was present in both cystic fibrosis (CF) and non-CF airway epithelia. Likewise, airway epithelia from wild-type mice and mice with disrupted genes for ClC-2 or ClC-3 all showed similar Cl− currents. These data suggest that the basolateral membrane of airway epithelia possesses a Cl− conductance that is not due to CFTR, ClC-2, or ClC-3. Its regulation by cAMP and PKC signaling pathways suggests that coordinated regulation of Cl− conductance in both apical and basolateral membranes may be important in controlling transepithelial Cl− movement.

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“…In the present study, we examined the effects of these three PAHs on the anion transport in human airway Calu-3 cells and demonstrated that only fluoranthene exposed to the apical membrane modulates the function. In polarized airway epithelia, HCO 3 Ϫ and Cl Ϫ secretion are primarily regulated by cytosolic cAMP and Ca 2ϩ , respectively (Smith and Welsh, 1992;Devor et al, 1999;Ito et al, 2001). Generally, the transepithelial anion transport is maintained through two steps: anion entry across the basolateral membrane and its export across the apical membrane (Ito et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…In polarized airway epithelia, HCO 3 Ϫ and Cl Ϫ secretion are primarily regulated by cytosolic cAMP and Ca 2ϩ , respectively (Smith and Welsh, 1992;Devor et al, 1999;Ito et al, 2001). Generally, the transepithelial anion transport is maintained through two steps: anion entry across the basolateral membrane and its export across the apical membrane (Ito et al, 2001). The apical CFTR is well known as a common pathway for HCO 3 Ϫ and Cl Ϫ export (Devor et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Effects of Fluoranthene, a Polycyclic Aromatic Hydrocarbon, on cAMP-Dependent Anion Secretion in Human Airway Epithelia

Ito¹,

Son²,

Sato³

et al. 2003

J Pharmacol Exp Ther

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The human respiratory tract is constantly exposed to polycyclic aromatic hydrocarbons (PAHs) through inhalation of atmospheric pollutants. We examined the effects of three PAHs (benzo[a]pyrene, anthracene, and fluoranthene) on the airway ion transport, which is essential for lung defense and normal airway function, using human airway epithelia (Calu-3). These three PAHs had no significant effect on the basal short-circuit current (I sc ). However, fluoranthene (1-100 M) applied in the apical compartment potentiated I sc in response to cAMP-related agents (isoproterenol, forskolin, and 8-bromo-cAMP). The effects of fluoranthene were unaffected by ellipticine, a PAH receptor antagonist. Estimation of the anionic composition of I sc revealed that isoproterenol increased both HCO 3 Ϫ and Cl Ϫ

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Novel Effects of Minocycline on Ca2+-Dependent Cl− Secretion in Human Airway Epithelial Calu-3 Cells

Cited by 13 publications

References 41 publications

Bisphenol A Inhibits Cl⁻ Secretion by Inhibition of Basolateral K⁺ Conductance in Human Airway Epithelial Cells

Bisphenol A Inhibits Cl⁻ Secretion by Inhibition of Basolateral K⁺ Conductance in Human Airway Epithelial Cells

Basolateral chloride current in human airway epithelia

Effects of Fluoranthene, a Polycyclic Aromatic Hydrocarbon, on cAMP-Dependent Anion Secretion in Human Airway Epithelia

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Novel Effects of Minocycline on Ca2+-Dependent Cl− Secretion in Human Airway Epithelial Calu-3 Cells

Cited by 13 publications

References 41 publications

Bisphenol A Inhibits Cl− Secretion by Inhibition of Basolateral K+ Conductance in Human Airway Epithelial Cells

Bisphenol A Inhibits Cl− Secretion by Inhibition of Basolateral K+ Conductance in Human Airway Epithelial Cells

Basolateral chloride current in human airway epithelia

Effects of Fluoranthene, a Polycyclic Aromatic Hydrocarbon, on cAMP-Dependent Anion Secretion in Human Airway Epithelia

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Bisphenol A Inhibits Cl⁻ Secretion by Inhibition of Basolateral K⁺ Conductance in Human Airway Epithelial Cells

Bisphenol A Inhibits Cl⁻ Secretion by Inhibition of Basolateral K⁺ Conductance in Human Airway Epithelial Cells