Novel electrochemical and electrochemiluminescence dual-modality sensing platform for sensitive determination of antimicrobial peptides based on probe encapsulated liposome and nanochannel array electrode

Abstract

With the increasing application of antimicrobial peptides (AMPs) to replace antibiotics in medicine, food and agriculture, it is highly desired to develop a fast, reliable, and convenient strategy for sensitive detection of AMPs. Herein, a novel electrochemical (EC) and electrochemiluminescence (ECL) dual-modality sensing platform was developed based on probe encapsulated liposomes and nanochannel array modified electrodes, which enables sensitive determination of nisin in food samples. The bifunctional probe with both EC and ECL signals, tris(2,2-bipyridyl) dichlororuthenium (II) (Ru(bpy)32+), was chosen to be easily encapsulated in liposomes (Ru(bpy)32+@liposome). Based on the unique sterilization mechanism that AMPs can disrupt cell membranes, Ru(bpy)32+@liposome can be destroyed by nisin and release a large number of Ru(bpy)32+ probes. Vertically-ordered mesoporous silica-nanochannel film (VMSF) modified ITO electrodes (VMSF/ITO) prepared by electrochemically assisted self-assembly (EASA) method were applied as the sensing electrode. Due to the efficient enrichment of Ru(bpy)32+ by the negatively charged nanochannel arrays, VMSF/ITO enables detection of the EC/ECL signals of the released Ru(bpy)32+ probes with ultrahigh sensitivity. In consequence, sensitive dual-modality detection of nisin was achieved by the combination of Ru(bpy)32+@liposome and VMSF/ITO. The developed sensing system can realize sensitive determination of nisin in ECL mode in the concentration range of 10 ng/ml to 50 μg/ml with a limit of detection (LOD) of 9.3 ng/ml, or in EC mode from 800 ng/ml to 100 μg/ml with a LOD of 70 ng/ml. Combined with the excellent anti-fouling and anti-interference performance of VMSF, rapid and sensitive detection of nisin in milk or egg white was also achieved by the sensor.

Highly Sensitive Electrochemical Detection of Paraquat in Environmental Water Samples Using a Vertically Ordered Mesoporous Silica Film and a Nanocarbon Composite

“…The pores were hexagonally aligned and very regular over a large domain. Cyclic voltammetry (CV) is considered to be an effective method to evaluate the intactness and permselectivity of VMSF [ 46 ]. Figure 2 b displays the CV curves of bare GCE, SM@VMSF/3DG-CNT/GCE and VMSF/3DG-CNT/GCE in a 0.05 M KHP solution containing 0.5 mM Ru(NH 3 ) 6 3+ .…”

Disposal Immunosensor for Sensitive Electrochemical Detection of Prostate-Specific Antigen Based on Amino-Rich Nanochannels Array-Modified Patterned Indium Tin Oxide Electrode

“…Moreover, the cost is low, and it can be prepared in batches in a large area, so it is an ideal electrode modification material. VMSF can be stably modified on indium tin oxide (ITO) electrodes [ 52 , 53 ]. This is attributed to the covalent bonding between VMSF and the ITO surface through the formation of -Si-O-In- or -Si-O-Sn- bonds.…”

Fabrication of a Ratiometric Fluorescence Sensor Based on Carbon Dots as Both Luminophores and Nanozymes for the Sensitive Detection of Hydrogen Peroxide

“…Nanomaterials usually exhibit novel optical/electrical/magnetic properties and have a high specific surface area due to their unique microstructure and nanoscale size [ 33 , 34 , 35 ]. In recent years, the use of various functional nanomaterials to construct novel sensing systems has attracted much attention [ 36 , 37 , 38 , 39 ].…”