Novel electrospun polycaprolactone/graphene oxide/Fe3O4 nanocomposites for biomedical applications

Aydoğdu, Mehmet Onur; Ekren, Nazmi; Süleymanoğlu, Mediha; Erdem‐Kuruca, Serap; Lin, Chun‐Cheng; Bulbul, Ertugrul; Erdol, Meltem Nur; Oktar, Faik N.; Terzi, Umit Kemalettin; Kılıç, Osman; Gündüz, Oğuzhan

doi:10.1016/j.colsurfb.2018.09.035

Cited by 41 publications

(14 citation statements)

References 29 publications

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“…It was observed that an entrapped of MNPs into nano-scaffold led to an increase the tensile strength, while its elongation was slightly reduced. Indeed, homogenous dispersion of metal-based MNPs acted as the backbone and improved the mechanical strength of nanocomposite scaffold along with high porosity [ 31 ]. In addition, the Col I network produces the thinner bridges between PCL polymers (Fig.…”

Section: Resultsmentioning

confidence: 99%

PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

et al. 2022

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Background The bone tissue engineering (BTE) approach has been introduced as an alternative to conventional treatments for large non-healing bone defects. Magnetism promotes stem cells' adherence to biocompatible scaffolds toward osteoblast differentiation. Furthermore, osteogenic differentiation media are expensive and any changes in its composition affect stem cells differentiation. Moreover, media growth factors possess a short half-life resulting in the rapid loss of their functions in vivo. With the above in mind, we fabricated a multilayered nanocomposite scaffold containing the wild type of Type I collagen (Col I) with endogenous magnetic property to promote osteogenesis in rat ADSCs with the minimum requirement of osteogenic differentiation medium. Methods Fe3O4 NPs were synthesized by co-precipitation method and characterized using SEM, VSM, and FTIR. Then, a PCL/Col I nanocomposite scaffold entrapping Fe3O4 NPs was fabricated by electrospinning and characterized using SEM, TEM, AFM, VSM, Contact Angle, tensile stretching, and FTIR. ADSCs were isolated from rat adipose tissue and identified by flow cytometry. ADSCs were loaded onto PCL/Col I and PCL/Col I/Fe3O4-scaffolds for 1–3 weeks with/without osteogenic media conditions. The cell viability, cell adhesion, and osteogenic differentiation were evaluated using MTT assay, SEM, DAPI staining, ALP/ARS staining, RT-PCR, and western blotting, respectively. Results SEM, VSM, and FTIR results indicated that Fe3O4 was synthesized in nano-sized (15–30 nm) particles with spherical-shaped morphology and superparamagnetic properties with approved chemical structure as FTIR revealed. According to SEM images, the fabricated magnetic scaffolds consisted of nanofiber (500–700 nm). TEM images have shown the Fe3O4 NPs entrapped in the scaffold's fiber without bead formation. FTIR spectra analysis confirmed the maintenance of the natural structure of Col I, PCL, and Fe3O4 upon electrospinning. AFM data have shown that MNPs incorporation introduced stripe-like topography to nanofibers, while the depth of the grooves has decreased from 800 to 500 nm. Flow cytometry confirmed the phenotype of ADSCs according to their surface markers (i.e., CD29 and CD105). Additionally, Fe3O4 NP improved nanocomposite scaffold strength, wettability, porosity, biocompatibility and also facilitates the ALP activity, calcium-mineralization. Finally, magnetic nanocomposite scaffolds upregulated osteogenic-related genes or proteins’ expression (e.g., Col I, Runx2, OCN, ON, BMP2) in seeded ADSCs with/without osteo-differentiation media conditions. Conclusions Together, these results indicate that Fe3O4 NPs within the natural structure of Col I increase osteogenic differentiation in osteogenic cues-free media conditions. This effect could be translated in vivo toward bone defects healing. These findings support the use of natural ECM materials alongside magnetic particles as composite scaffolds to achieve their full therapeutic potential in BTE treatments. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

et al. 2022

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“…In relation to connective tissue engineering, cell viability of 3T3 fibroblasts was at 103%, and Saos-2 osteoblasts cell viability was at 105% when exposed to GO/PCL/Fe 3 O 4 scaffolds. However, as GO concentration increased, total cell viability decreased by up to 21.5% . In contrast, GO in a higher concentration was seen to be optimum for the growth of ATDC5 cells in cartilage tissue engineering.…”

Section: Tissue Engineeringmentioning

confidence: 91%

“…The inclusion of GO improved hydrophilicity of the scaffolds. ,, Biocompatibility of the structure was shown to be unaltered in relation to MG63 cells, while it was improved in other studies using human osteosarcoma cells (HOS), MG63 cells, bone marrow mesenchymal stem cells (BMSCs), and C2C12 cells . Cell attachment and proliferation were enhanced with the inclusion of GO in scaffolds. ,− ,,− Osteogenic expression and differentiation were seen to have improved ,,,, along with mineral deposition. ,, The enzyme expression rate was enhanced, with regard to alkaline phosphatase. , Mechanical properties of scaffolds improved immensely with the inclusion of GO, ,− ,,, with elongation improving by 462% and tensile strength by up to 230% in a particular case . Novel biphasic GO-containing electrospun composites have been manufactured for their use as a biomedical implant.…”

Section: Tissue Engineeringmentioning

confidence: 93%

Biomedical Applications of Electrospun Graphene Oxide

Grant

Pillai

Hehir

et al. 2021

ACS Biomater. Sci. Eng.

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Graphene oxide (GO) has broad potential in the biomedical sector. The oxygen-abundant nature of GO means the material is hydrophilic and readily dispersible in water. GO has also been known to improve cell proliferation, drug loading, and antimicrobial properties of composites. Electrospun composites likewise have great potential for biomedical applications because they are generally biocompatible and bioresorbable, possess low immune rejection risk, and can mimic the structure of the extracellular matrix. In the current review, GO-containing electrospun composites for tissue engineering applications are described in detail. In addition, electrospun GO-containing materials for their use in drug and gene delivery, wound healing, and biomaterials/medical devices have been examined. Good biocompatibility and anionic-exchange properties of GO make it an ideal candidate for drug and gene delivery systems. Drug/gene delivery applications for electrospun GO composites are described with a number of examples. Various systems using electrospun GO-containing therapeutics have been compared for their potential uses in cancer therapy. Micro- to nanosized electrospun fibers for wound healing applications and antimicrobial applications are explained in detail. Applications of various GO-containing electrospun composite materials for medical device applications are listed. It is concluded that the electrospun GO materials will find a broad range of biomedical applications such as cardiac patches, medical device coatings, sensors, and triboelectric nanogenerators for motion sensing and biosensing.

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“…Wen et al 44 synthesized PCL/Fe 3 O 4 fibrous catalyst by electrospinning technology and studied the catalytic efficiency and reusable rate of the porous catalyst. Aydogdu et al 45 synthesized PCL/Graphene Oxide/Fe 3 O 4 nanocomposite fibers and used as biocompatible scaffolds for biomedical applications. Zhang et al 46 fabricated creatively PCL/Fe 3 O 4 nanocomposites by using Fe 3 O 4 with average diameter of ~80 nm through melting method.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of poly(ε‐caprolactone)/ Fe ₃ O ₄ nanocomposites

Liu

Chen

et al. 2021

Polymer Crystallization

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Poly (ε‐caprolactone) (PCL)/Fe3O4 nanocomposites were prepared via solvent casting method. Microstructure, composition, and crystal structure of the nanocomposite were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, and X‐ray diffraction, respectively. The effects of the content of nano‐Fe3O4 on PCL matrix non‐isothermal and isothermal crystallization behavior were analyzed by differential scanning calorimeter and polarizing microscopy, respectively, and, the thermal stability of nanocomposites was tested by using thermogravimetric analyzer. The results revealed that Fe3O4 nanoparticles could be dispersed uniformly in PCL matrix. Crystal structure of the polymer matrix was not changed and a handful of nanoparticles has obvious nucleation effect, while excessive particles hindered the crystallization of PCL matrix. However, the thermal decomposition stability of nanocomposites decreased by the addition of Fe3O4 particles.

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Novel electrospun polycaprolactone/graphene oxide/Fe3O4 nanocomposites for biomedical applications

Cited by 41 publications

References 29 publications

PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

Biomedical Applications of Electrospun Graphene Oxide

Preparation and characterization of poly(ε‐caprolactone)/ Fe ₃ O ₄ nanocomposites

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Novel electrospun polycaprolactone/graphene oxide/Fe3O4 nanocomposites for biomedical applications

Cited by 41 publications

References 29 publications

PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

Biomedical Applications of Electrospun Graphene Oxide

Preparation and characterization of poly(ε‐caprolactone)/ Fe 3 O 4 nanocomposites

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Preparation and characterization of poly(ε‐caprolactone)/ Fe ₃ O ₄ nanocomposites