Novel endo-(1,4)-β-glucanase Bgh12A and xyloglucanase Xgh12B from Aspergillus cervinus belong to GH12 subgroup I and II, respectively

Rykov, S. V.; Kornberger, Petra; Herlet, Jonathan; Tsurin, Nikita V.; Зоров, И. Н.; Zverlov, Vladimir V.; Liebl, Wolfgang; Schwarz, Wolfgang H.; Yarotsky, S. V.; Березина, О. В.

doi:10.1007/s00253-019-10006-x

Cited by 13 publications

(12 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Xyloglucanases have attracted significant attention due to their application in the food and feed industries (Hu et al, 2013; Rashmi & Siddalingamurthy, 2018). Some xyloglucanases have been expressed in P. pastoris ; however, the expression levels are generally low (Berezina et al, 2017; Rykov et al, 2019). In this study, high‐cell‐density fermentation produced the maximal activity of RmXEG12B with 26,200 U/mL (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Many fungal xyloglucanases are optimally active in the pH range of 4.0–6.0 (Master et al, 2008; Rykov et al, 2019; Xian et al, 2016). RmXEG12B exhibited an optimal acidic pH of 5.0 (Figure 3a), which is the same as that expressed in Escherichia coli (pH 5.0), and the xyloglucanase from A. cervinus (pH 5.0) (Rykov et al, 2019; Song et al, 2013). However, RmXEG12B was stable within a wider pH range (pH of 3.0–10.0; Figure 3b) than that expressed in E. coli (stable within a pH of 4.0–8.0) and PoxXEG12A from Penicillium oxalicum (stable within a pH 3.5–7.0) (Song et al, 2013; Xian et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…It is important to develop an effective method for xyloglucanase production due to the low level for industrial application (Pol et al, 2012; Yaoi & Mitsuishi, 2004). Several xyloglucanases have been expressed in Pichia pastoris (Berezina et al, 2017; Rykov et al, 2019). However, only RmXEG12A has been expressed at high level (25,700 U/mL), which may be sufficient for commercial production (Wang, Li et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

High‐level expression of xyloglucanase B from Rhizomucor miehei and its application in the preparation of partially hydrolyzed apple pomace xyloglucan

Wang

Zhu

et al. 2022

Food Bioengineering

View full text Add to dashboard Cite

A xyloglucanase gene (RmXEG12B) was cloned from Rhizomucor miehei CAU432 and successfully expressed in Pichia pastoris. The highest xyloglucanase activity of 26,200 U/mL was achieved after 168 h high-cell-density fermentation. The optimal pH and temperature of RmXEG12B were 5.0 and 55°C, respectively. RmXEG12B showed good stability within pH of 3.0-10.0 and was stable up to 50°C. It exhibited the highest specific activity (5989.3 U/mg) towards tamarind xyloglucan. RmXEG12B hydrolyzed apple pomace xyloglucan to produce the partially hydrolyzed apple pomace xyloglucan, which demonstrated a weight average molecular weight of 1.6 × 10 4 Da and contained three major oligosaccharides with a degree of polymerization of 7-10. This is a strategy for the comprehensive utilization of apple pomace to produce the partially hydrolyzed apple pomace xyloglucan.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High‐level expression of xyloglucanase B from Rhizomucor miehei and its application in the preparation of partially hydrolyzed apple pomace xyloglucan

Wang

Zhu

et al. 2022

Food Bioengineering

View full text Add to dashboard Cite

show abstract

“…Generally, XEG activities are significant towards xyloglucan; however, many XEGs belonging to GH12 and GH74 have low or no activity towards CMC, MCC, lichenan, glucomannan, and xylan (Desmet et al 2007;Gloster et al 2007;Sato et al 2016;Takeda et al 2013). Highly xyloglucan-specific GH12 XEGs have been isolated from various fungi including Aspergillus niveus (Damásio et al 2012), Aspergillus terreus (Vitcosque et al 2016), Aspergillus niger (Master et al 2008), and Aspergillus cervinus (Rykov et al 2019). Unlike xyloglucan-specific GH12 XEGs, some fungal GH12 XEGs with broad substrate specificity have also been reported (Segato et al 2017;Yang et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Characterization of FsXEG12A from the cellulose-degrading ectosymbiotic fungus Fusarium spp. strain EI cultured by the ambrosia beetle

et al. 2020

View full text Add to dashboard Cite

Despite the threat of Fusarium dieback posed due to ambrosia fungi cultured by ambrosia beetles such as Euwallacea spp., the wood-degradation mechanisms utilized by ambrosia fungi are not fully understood. In this study, we analyzed the 16S rRNA and 18S rRNA genes of the microbial community from the Ficus tree tunnel excavated by Euwallacea interjectus and isolated the cellulose-degrading fungus, Fusarium spp. strain EI, by enrichment culture with carboxymethyl cellulose as the sole carbon source. The cellulolytic enzyme secreted by the fungus was identified and expressed in Pichia pastoris, and its enzymatic properties were characterized. The cellulolytic enzyme, termed FsX-EG12A, could hydrolyze carboxymethyl cellulose, microcrystalline cellulose, xyloglucan, lichenan, and glucomannan, indicating that the broad substrate specificity of FsXEG12A could be beneficial for degrading complex wood components such as cellulose, xyloglucan, and galactoglucomannan in angiosperms. Inhibition of FsXEG12A function is, thus, an effective target for Fusarium dieback caused by Euwallacea spp.

show abstract

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

Harvey

2023

Mass Spectrometry Reviews

View full text Add to dashboard Cite

This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2020. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. The review is basically divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of arrays. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other areas such as medicine, industrial processes and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. The reported work shows increasing use of incorporation of new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented nearly 40 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show little sign of diminishing.

show abstract

Novel endo-(1,4)-β-glucanase Bgh12A and xyloglucanase Xgh12B from Aspergillus cervinus belong to GH12 subgroup I and II, respectively

Cited by 13 publications

References 62 publications

High‐level expression of xyloglucanase B from Rhizomucor miehei and its application in the preparation of partially hydrolyzed apple pomace xyloglucan

High‐level expression of xyloglucanase B from Rhizomucor miehei and its application in the preparation of partially hydrolyzed apple pomace xyloglucan

Characterization of FsXEG12A from the cellulose-degrading ectosymbiotic fungus Fusarium spp. strain EI cultured by the ambrosia beetle

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

Contact Info

Product

Resources

About