Novel Endophyte Technology: Selection of the Fungus

West, Charles P.; Marlatt, M. L.; McConnell, Megan; Piper, E. L.; Kring, T. J.

doi:10.2135/cssaspecpub26.c8

Cited by 3 publications

(1 citation statement)

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“…Plants P27CTE+ and P46CTE+ were derived from locally obtained KY31 seeds harboring E. coenophiala strain CTE‐1 (Young et al, 2014). Two plants with NTE strains were obtained by inoculation of tall fescue cultivar KY31 E− seedlings with different fungal strains from L. arundinaceum plants collected in Morocco (West et al, 1998), one of which was a strain (designated NTE‐19) of E. coenophiala , and the other was a strain (designated FaTG4‐16) of the E. typhina × Epichloë sp. hybrid species FaTG‐4 (Takach and Young, 2014).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis and Differential Expression in Tall Fescue Harboring Different Endophyte Strains in Response to Water Deficit

Dinkins

Nagabhyru²,

Young

et al. 2019

The Plant Genome

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Core Ideas RNA‐seq was performed on four tall fescue clone pairs. Three Epichloë coenophiala strains were evaluated. Gene expression was compared for stressed and unstressed plants. Differentially expressed unigenes were identified. Few positive endophyte effects on stress tolerance were observed. Two tall fescue [Lolium arundinaceum (Schreb.) Darbysh. = Schedonorus arundinaceus (Schreb.) Dumort. = Festuca arundinacea var. arundinacea Schreb.] plant genotypes with an Epichloë coenophiala (Morgan‐Jones & W. Gams) C.W. Bacon & Schardl common toxic endophyte (CTE), one with a nontoxic strain (NTE19) and one with another Epichloë species (FaTG‐4) were evaluated and compared with their respective endophyte‐free clones for responses to water‐deficit stress in the greenhouse. One of the plant genotypes (P27) showed a positive effect of its CTE strain on tiller production after stress and resumed watering. In transcriptome analysis of the pseudostems (leaf sheath whorls), differentially expressed genes (DEGs) were defined as having at least twofold expression difference and false discovery rate (FDR) < 0.05 in comparisons of water treatment (stressed or watered), endophyte presence or absence, or both. Stress affected 38% of the plant transcripts including those for the expected stress‐response pathways. The DEGs affected by endophyte in stressed plants were unique to individual plant genotypes. In unstressed plants, endophyte presence tended to reduce expression of genes putatively for defense against fungi, but in unstressed P27 endophyte presence there was enhanced expression of dehydrin and heat shock protein genes. Our results indicated subtle and variable effects of endophytes on tall fescue gene expression; where the endophyte confers protection, its effects on plant gene expression may help prime the plant for stress resistance.

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Section: Methodsmentioning

confidence: 99%