Novel Endophytic Trichoderma spp. Isolated from  Healthy Coffea arabica Roots are Capable of Controlling  Coffee Tracheomycosis

Mulaw, Temesgen Belayneh; Druzhinina, Irina S.; Kubicek, Christian P.; Atanasova, Lea

doi:10.3390/d5040750

Cited by 50 publications

(23 citation statements)

References 52 publications

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“…The latter contradicts studies targeting Coffea arabica, for which Trichoderma was shown to inhabit its rhizosphere but was not recovered as a leaf endophyte (Mulaw et al, 2010;Vega et al, 2010;Mulaw et al, 2013). However, these other studies were conducted in different geographic regions and following different protocols.…”

Section: Discussionmentioning

confidence: 82%

Wild trees in the Amazon basin harbor a great diversity of beneficial endosymbiotic fungi: is this evidence of protective mutualism?

Gazis

Chaverri

2015

Fungal Ecology

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Hevea Natural rubberTropical forest conservation a b s t r a c t It has been shown that the disappearance of, or drastic changes in, ancestral and indigenous (or native) endosymbiotic microbiota can lead to many adverse health consequences.However, the effects of changes in beneficial endosymbionts in plants are poorly known (except for mycorrhizal and rhizobial associations). We sampled and compared endophytes from hundreds of trees belonging to the economically important genus Hevea, the source of natural rubber, in their native range in the Amazon basin and in plantations. We also conducted antagonism tests to determine the potential effects that some of these endophytes may have on selected plant pathogenic fungi. The natural and indigenous endosymbiotic mycota of the rubber tree (Hevea) contains a high diversity of beneficial fungi that may protect against pathogens (protective mutualism). In contrast, plantation trees have a reduced and different diversity of these beneficial fungi. We propose that abundance, and not just presence, of competitive fungal strains and species (i.e., Trichoderma and Tolypocladium) create a protective effect against pathogens in wild trees. This study provides support for the importance of mutualistic endosymbionts in plant health and ecosystem resilience, and calls for awareness of their potential loss by human-related activities.ª

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Section: Discussionmentioning

confidence: 82%

Wild trees in the Amazon basin harbor a great diversity of beneficial endosymbiotic fungi: is this evidence of protective mutualism?

Gazis

Chaverri

2015

Fungal Ecology

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“…yang diisolasi dari akar Coffee arabica yang mampu mengendalikan tracheomycosis pada kopi (Mulaw et al, 2013) Hasil analisis sekuens ITS DNA berdasarkan database pada program BLAST-N menunjukkan, bahwa isolat Swn-1, isolat Swn-2 dan isolat Psr-1 mempunyai hubungan kekerabatan dengan Trichoderma harzianum, isolat Ksn dengan Trichoderma asperellum, isolat Psr-2 dengan Trichoderma gamsii dan isolat Psr-3 dengan Trichoderma koningiopsis, dengan tingkat kemiripan 99 % (Tabel 2).…”

Section: Hasil Dan Pembahasanunclassified

Eksplorasi Dan Identifikasi Trichoderma Endofitik Pada Pisang

Taribuka¹,

Sumardiyono²,

Widyastuti³

et al. 2017

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.

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“…17 Diverse Trichoderma species were also found among coffee endophytic fungi that colonize healthy tissues. 18,19 Trichoderma population was enriched in coffee waste containing caffeine »0.1% in wet weight. 20,21 These observation suggest a specific relationship between caffeine and Trichoderma.…”

Section: Introductionmentioning

confidence: 99%

Caffeine fostering of mycoparasitic fungi against phytopathogens

Sugiyama

Sano

Yazaki

et al. 2016

Plant Signaling & Behavior

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Abbreviation: PDA, potato dextrose agar Caffeine (1,3,7-trimethixanthine) is a typical purine alkaloid produced in more than 80 plant species. Its biological role is considered to strengthen plant's defense capabilities, directly as a toxicant to biotic attackers (allelopathy) and indirectly as an activator of defense system (priming). Caffeine is actively secreted into rhizosphere through primary root, and possibly affects the structure of microbe community nearby. The fungal community in coffee plant rhizosphere is enriched with particular species, including Trichoderma family, a mycoparasite that attacks and kills phytopathogens by coiling and destroying their hyphae. In the present study, the caffeine response of 8 filamentous fungi, 4 mycoparasitic Trichoderma, and 4 prey phytopathogens, was examined. Results showed that allelopathic effect of caffeine on fungal growth and development was differential, being stronger on pathogens than on Trichoderma species. Upon confronting, the prey immediately ceased the growth, whereas the predator continued to grow, indicating active mycoparasitism to have occurred. Caffeine enhanced mycoparasitism up to 1.7-fold. Caffeine thus functions in a double-track manner against fungal pathogens: first by direct suppression of growth and development, and second by assisting their natural enemy. These observations suggest that caffeine is a powerful weapon in the arms race between plants and pathogens by fostering enemy's enemy, and we propose the idea of "caffeine fostering" as the third role of caffeine.

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Novel Endophytic Trichoderma spp. Isolated from Healthy Coffea arabica Roots are Capable of Controlling Coffee Tracheomycosis

Cited by 50 publications

References 52 publications

Wild trees in the Amazon basin harbor a great diversity of beneficial endosymbiotic fungi: is this evidence of protective mutualism?

Wild trees in the Amazon basin harbor a great diversity of beneficial endosymbiotic fungi: is this evidence of protective mutualism?

Eksplorasi Dan Identifikasi Trichoderma Endofitik Pada Pisang

Caffeine fostering of mycoparasitic fungi against phytopathogens

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