Novel extracellular synthesized silver nanoparticles using thermophilic
            <i>Anoxybacillus flavithermus</i>
            and
            <i>Geobacillus stearothermophilus</i>
            and their evaluation as nanodrugs

Giray, Gülay; Gonca, Serpil; Özdemir, Sadin; Işık, Zelal; Yılmaz, Erkan; Soylak, Mustafa; Dizge, Nadir

doi:10.1080/10826068.2023.2230496

Cited by 1 publication

(1 citation statement)

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“…One approach is to employ biological synthesis methods to reduce adverse effects [ 9 ]. In the previous works, it was shown that bacteria of the genus Geobacillus can synthesize AgNPs [ 10 , 11 , 12 ]. AgNPs obtained via chemical or physical synthesis are well-described substances used for various antifungal and antibacterial purposes.…”

Section: Introductionmentioning

confidence: 99%

Induction of Apoptosis with Silver Nanoparticles Obtained Using Thermophilic Bacteria

Čekuolytė,

Šapaitė,

Žemgulytė

et al. 2024

JFB

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Yeasts resistant to antifungals have become an increasing risk to human health. One of the best antimicrobial properties is reported to be present in silver nanoparticles (AgNPs); however, little is known about the antimicrobial potential of AgNPs produced using thermophilic bacteria. How AgNPs cause cell death is different depending on the type of the cell, and the mode of death induced is cell-type specific. Apoptosis, one of the types of regulated cell death, can be extremely useful in the fight against infection because surrounding cells that have phagocytic activity can efficiently absorb the apoptotic bodies formed during apoptosis. In the course of this work, for the first time, comprehensive antifungal studies of AgNPs were performed using thermophilic Geobacillus spp. bacteria against Candida guilliermondii, also with the addition of the model yeast Saccharomyces cerevisiae. The determined minimal inhibitory concentrations (MICs) were 10 μg/mL against C. guilliermondii and 50 μg/mL against S. cerevisiae for Geobacillus sp. strain 25 AgNPs, and for Geobacillus sp. 612 the MICs were 5 μg/mL and 25 μg/mL, respectively. It was shown for the first time that the exposure of the yeast cells leads to caspase activation in both S. cerevisiae and C. guilliermondii after exposure to Geobacillus spp. AgNPs. Also, a statistically significant change in the number of cells with permeable membranes was detected. Moreover, it was shown that the antimicrobial effect of the AgNPs is related to ROS generation and lipid peroxidation in C. guilliermondii yeast.

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