Novel fabrication of fluorescent silk utilized in biotechnological and medical applications

Kim, Dong Wook; Lee, Ok Joo; Kim, Seong‐Wan; Ki, Chang Seok; Chao, Janet Ren; Yoo, Hyojong; Yoon, Sung‐il; Lee, Jeong Eun; Park, Ye Ri; Kweon, HaeYong; Lee, Kwang Gill; Kaplan, David L.; Park, Chan Hum

doi:10.1016/j.biomaterials.2015.08.025

Cited by 59 publications

(40 citation statements)

References 20 publications

(18 reference statements)

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“…In addition, the fluorescent silk fibroin sponges are implanted subcutaneously in rats and all of them retain fluorescence 7 d later, which indicates long‐term cytocompatibility of the fluorescent silk fibroin sponges. Therefore, the fluorescent silk fibroins can be utilized as a bioimaging tool in clinic by conjugating with biomarkers ( Figure ) . Genetic engineering technique exemplifies a sustainable route to control naturally occurring proteins at the gene level, which is crucial for spider silk manufacturing as well.…”

Section: Multilevel Modificationmentioning

confidence: 99%

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Engineering the Future of Silk Materials through Advanced Manufacturing

et al. 2018

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Silk is a natural fiber renowned for its outstanding mechanical properties that have enabled the manufacturing of ultralight and ultrastrong textiles. Recent advances in silk processing and manufacturing have underpinned a re-interpretation of silk from textiles to technological materials. Here, it is argued that silk materials-optimized by selective pressure to work in the environment at the biotic-abiotic interface-can be harnessed by human micro- and nanomanufacturing technology to impart new functionalities and opportunities. A critical overview of recent progress in silk technology is presented with emphasis on high-tech applications enabled by recent innovations in multilevel modifications, multiscale manufacturing, and multimodal characterization of silk materials. These advances have enabled successful demonstrations of silk materials across several disciplines, including tissue engineering, drug delivery, implantable medical devices, and biodissolvable/degradable devices.

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Section: Multilevel Modificationmentioning

confidence: 99%

“…The fluorescent cocoons produced by transgenic silkworms had various fluorescent proteins including EGFP, mKate2, and EYFP. D) Whole‐body imaging of fluorescent silk fibroin sponges in the dorsal region of a nude mouse at 7 d. Reproduced with permission . Copyright 2015, Elsevier Ltd.…”

Section: Multilevel Modificationmentioning

confidence: 99%

Engineering the Future of Silk Materials through Advanced Manufacturing

et al. 2018

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“…Fluorescent silk, one of the novel natural functional biomaterials, is gaining enormous attention due to its great potential for biomedical and intelligent textile-related applications [1][2][3][4][5]. It has been reported that fluorescence can be imparted through the incorporation of various organic dyes and inorganic nanoparticles into silk through post-dyeing of naturally produced silk [6,7].…”

Section: Introductionmentioning

confidence: 99%

Super-strong and Intrinsically Fluorescent Silkworm Silk from Carbon Nanodots Feeding

et al. 2019

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HIGHLIGHTS • Intrinsically, super-strong fluorescent silk was fabricated via feeding Bombyx mori silkworms with carbon nanodots. • The multi-functional silks showed no cytotoxicity to Schwann cells and exhibited great potential in bioimaging. • The reinforcing mechanism of multi-functional silks was proposed. ABSTRACT Fluorescent silk is fundamentally important for the development of future tissue engineering scaffolds. Despite great progress in the preparation of a variety of colored silks, fluorescent silk with enhanced mechanical properties has yet to be explored. In this study, we report on the fabrication of intrinsically super-strong fluorescent silk by feeding Bombyx mori silkworm carbon nanodots (CNDs). The CNDs were incorporated into silk fibroin, hindering the conformation transformation, confining crystallization, and inducing orientation of mesophase. The resultant silk exhibited super-strong mechanical properties with breaking strength of 521.9 ± 82.7 MPa and breaking elongation of 19.2 ± 4.3%, improvements of 55.1% and 53.6%, respectively, in comparison with regular silk. The CNDs-reinforced silk displayed intrinsic blue fluorescence when exposed to 405 nm laser and exhibited no cytotoxic effect on cells, suggesting that multi-functional silks would be potentially useful in bioimaging and other applications.

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“…In their native environment, B. mori spin, via pultrusion, a dual filament fiber composed primarily of the protein fibroin, coated in layers of a secondary protein known as sericin, which acts as a lubricant in the duct and a binder in the cocoon, a non‐woven composite structure . While B. mori's natural fiber mechanical properties are less desirable than those of, say the dragline filaments of Nephila edulis , there is abundant evidence to suggest that they can be further processed to comparable performance levels and their generally more abundant, accessible nature makes them currently a more practical technology platform …”

mentioning

confidence: 99%

The Energy Requirements for Flow‐Induced Solidification of Silk

Sparkes

Holland

2018

Macromolecular Bioscience

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Natural silk spinning has undergone strong selection for resource efficiency and thus presents a biomimetic ideal for fiber production. Industrial replication of natural silk fibers would enable access to low energy, cost-efficient processing, but is hampered by a lack of understanding surrounding the conversion of liquid feedstock into a solid fiber as a result of flow. Previously, shear stress, shear rate, or time have been presented as criteria for silk fiber formation, but here it is proposed that spinning requires carefully balancing all three, and is a result of controlled energy accumulation in the feedstock. To support this hypothesis, rheology is used to probe the energy required for conversion, compare differences between amorphous solid and ordered fiber production and explain the energetic penalty the latter demands. New definitions of what constitutes an artificial silk fiber are discussed, along with methods to ensure that each spinning criterion is met during biomimetic spinning.

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Novel fabrication of fluorescent silk utilized in biotechnological and medical applications

Cited by 59 publications

References 20 publications

Engineering the Future of Silk Materials through Advanced Manufacturing

Engineering the Future of Silk Materials through Advanced Manufacturing

Super-strong and Intrinsically Fluorescent Silkworm Silk from Carbon Nanodots Feeding

The Energy Requirements for Flow‐Induced Solidification of Silk

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