Novel fluorescence-photochrome labeling method in the study of biomembrane dynamics

Likhtenshtein, Gertz I.; Bishara, Rizak; Papper, Vladislav; Uzan, B.; Fishov, Itzhak; Gill, D.; Parola, Abraham H.

doi:10.1016/s0165-022x(96)00022-x

Cited by 28 publications

(28 citation statements)

References 14 publications

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“…The following reactants were prepared by slight modification of our procedure that we published in [6]: trans-4-N,N′-dimethylamino-4′-nitrostilbene, trans-4-methoxy-4′-nitrostilbene, trans-4-N,N′ -dimethylamino-4′ -Naminostilbene and trans-4-methoxy-4′-N-aminostilbene.…”

Section: Synthesismentioning

confidence: 99%

“…We have previously provided clear evidence that the trans-cis photoisomerisation of stilbene compounds is very sensitive to medium microviscosity and can be used in biomedical applications [5][6][7][8]. The new fluorescence-photochrome method, developed by us, was used for investigation of local medium effects and phase transitions in biological membranes and on solid surfaces [5][6][7][8]. The method is based on monitoring the trans-cis photoisomerisation kinetics of stilbenes incorporated into an object of interest.…”

Section: Introductionmentioning

confidence: 99%

“…The method is based on monitoring the trans-cis photoisomerisation kinetics of stilbenes incorporated into an object of interest. It has been shown that the apparent rate constant of the trans-cis photoisomerisation in a relatively viscous media, for instance, in biological membranes, is controlled by the medium relaxation rate [6]. This method was also applied to studies of the microviscosity effects on the trans-cis photoisomerisation of the stilbene molecules immobilized onto quartz plates coated with lysozyme [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Novel Photochrome Aptamer Switch Assay (PHASA) for Adaptive Binding to Aptamers

Papper

Pokholenko

et al. 2014

J Fluoresc

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A novel Photochrome-Aptamer Switch Assay (PHASA) for the detection and quantification of small environmentally important molecules such as toxins, explosives, drugs and pollutants, which are difficult to detect using antibodies-based assays with high sensitivity and specificity, has been developed. The assay is based on the conjugation of a particular stilbene-analyte derivative to any aptamer of interest. A unique feature of the stilbene molecule is its reporting power via trans-cis photoisomerisation (from fluorescent trans-isomer to non-fluorescent cis-isomer) upon irradiation with the excitation light. The resulting fluorescence decay rate for the trans-isomer of the stilbene-analyte depends on viscosity and spatial freedom to rotate in the surrounding medium and can be used to indicate the presence of the analyte. Quantification of the assay is achieved by calibration of the fluorescence decay rate for the amount of the tested analyte. Two different formats of PHASA have been recently developed: direct conjugation and adaptive binding. New stilbene-maleimide derivatives used in the adaptive binding format have been prepared and characterised. They demonstrate effective binding to the model thiol compound and to the thiolated Malachite Green aptamer.

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Section: Synthesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel Photochrome Aptamer Switch Assay (PHASA) for Adaptive Binding to Aptamers

Papper

Pokholenko

et al. 2014

J Fluoresc

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“…Thus, by measuring the kinetic photoisomerization of the stilbene derivative molecules and proper calibration, one can acquire information about the microviscosity and steric hindrance of their surrounding environment. This method was used for the measurement fluidity of biological membranes [2,[31][32][33][34]. Information about the microviscosity of the binding site region is obtained when the label is bound to a specific site of a protein (as for an antigen, in a binding site of an antibody, or for a substrate, in the binding site of an enzyme, etc.)…”

Section: Fluorescence-photochrome Probesmentioning

confidence: 99%

Novel Fluorescent Methods for Biotechnological and Biomedical Sensoring: Assessing Antioxidants, Reactive Radicals, NO Dynamics, Immunoassay, and Biomembranes Fluidity

Likhtenshtein

2008

Appl Biochem Biotechnol

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We proposed and developed a series of fluorescent methods for analysis and investigation of biological systems with a view of future biotechnological and biomedical applications. The methods we describe have been built upon several photochemical and photophysical phenomena including fluorescent quenching, photochrome photoisomerization, and energy transfer. Three new types of molecular probes have been developed and employed for such studies: (1) dual fluorophore-nitroxide compounds, (2) fluorescence-photochrome molecules, and (3) super molecules containing both fluorescence and fluorescent quenching segments. The fluorescent properties of the new probes were intensively exploited for several practical applications including a real-time analysis of antioxidants, nitric oxide, superoxide, reactive radicals, trinitrotoluene, and metal ions, investigation of molecular dynamics of biomembranes in a wide range characteristic times, detection of protein conformational transition, and characterization of surface system. Owning high sensitivity, simplicity, and availability of fluorescent techniques, these methods can be widely employed and are adaptable to fibrooptic sensoring. A general survey of the physical principles and application of the new fluorescent methods has been provided.

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“…Moreover, they possess absorption and fluorescence properties similar to typical biochemical probes that can be monitored by simple optics. In addition, the quantitative study of direct and sensitized photoisomerization of stilbenes opens up new possibilities for the measurement of rotational and translational diffusion, both of which depend on molecular dynamics and steric hindrance in the vicinity of the probe (17)(18)(19).…”

mentioning

confidence: 99%