Novel fucolipids of human adenocarcinoma: characterization of the major Ley antigen of human adenocarcinoma as trifucosylnonaosyl Ley glycolipid (III3FucV3FucVI2FucnLc6).

Immunotherapy for cancer: synthetic carbohydrate-based vaccines

“…The KH-1 antigen, which displays the heterodimeric Le y -Le x heptasaccharide, was isolated from human colonic adenocarcinoma cells. 38 This antigen has only been found on the surface of these cells and has never been isolated from normal colonic tissue, thus providing a highly specific marker for malignancies. 39,40 The blood group precursors, Tn, STn, and TF-antigens, are the result of incomplete O-glycan synthesis.…”

Lewis X and Sialyl Lewis X Glycosphingolipids.

“…Multiple Lex and sLex GSLs have been isolated and identified from colorectal adenocarcinomas, although the expression and relative distribution of individual Lex (and Ley) species may vary between different tumors (21,53,54,56). In the neutral GSL fraction (42,54), Lex species include Lex-5 (A), dimeric Lex-8 (F) and trimeric Lex-11(L).…”

Sen-itiroh Hakomori and tumor-associated glycosphingolipids: an astoundingly productive decade, 1980–1990

“…This process involved huge amounts of starting tissue, and lengthy crude sub-fractionations of lipids, and culminated in fine separation on HPTLC plates using various MoAb overlays to detect active fractions. The final result was recovery of a homogenous trifucosylated Le y GSL, and the development of MoAbs indicating recognition of the internal epitope, and a putative probe as a tumor-associated cancer target [19] [20].…”