Novel genetic male sterility developed in (Capsicum annuum x C. chinense) x C. pubescens and induced by HNO2 showing Mendelian inheritance and aborted at telophase of microspore mother cell stage

Abstract

ABSTRACT.A novel genetic male sterile germplasm was developed by successively crossing of (C. annuum x C. chinense) x C. pubescens and by chemical mutagenesis in pepper. The sterile anthers showed morphological abnormalities, but pistils developed normally with fine pollination capability. We investigated fertility segregation through sibcrossing of the same strains and test crossing by male sterile plants with 6 advanced inbred lines. The results showed that male fertility in the pepper was dominant in the F 1 generation and segregated at a rate of 3:1 in the F 2 generation, suggesting that monogenic male sterility 3319 Breeding and characteristics of novel genetic male sterility ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 3318-3329 (2015) was recessive and conformed to Mendelian inheritance. Cyto-anatomy analysis revealed that microspore abortion of sterile anthers occurred during telophase in the microspore mother cell stage when tapetal cells showed excessive vacuolation, resulting in occupation of the loculi. The microspore mother cells self-destructed and autolyzed with the tapetum so that meiosis in pollen mother cells could not proceed past the tetrad stage.

Characterizing and marker-assisting a novel chili pepper (Capsicum annuum L.) yellow bud mutant with cytoplasmic male sterility

“…When pepper male sterility was first reported, multiple alleles of the restoring gene (Rf) were found (Peterson, 1958). Male sterile plants are common in nature where the interaction between genotype and environment determines plant fertility (Peterson, 1958;Huang et al, 2015). CMS causes mitochondrial-nuclear interactions with restorer genes in the nucleus, which can restore normal pollen production in CMS plants.…”

A 163‐bp insertion in the Capana10g000198 encoding a MYB transcription factor causes male sterility in pepper (Capsicum annuum L.)

“…Tetrads are not observed because the pollen sac is filled with necrotic residue. Instead of pollen grains, there is a dense belt in the empty locule (Huang et al., 2015). In this study, although the msc‐3 mutant shows no pollen grain in the mature anthers, similar to the above three MS mutants, the histological and anatomic characteristics of msc‐3 anthers differ from those of the other three mutants.…”

“…To date, a total of more than 20 GMS mutants, including ms1 to ms4 , ms6 to ms15 , msc‐1 , msc‐2 (Wang & Bosland, 2006), msk (Lee et al., 2010; Shifriss, 1973), ms w (Naresh et al., 2018), the GMS mutant from NW1 bell pepper (Lee et al., 2012) and the GMS mutant developed by interspecific hybridization and then by chemical mutagenesis (Huang et al., 2015), are documented in pepper. The majority of the above‐mentioned pepper GMS mutants are controlled by single recessive genes.…”

Genetic Resources for Fertility Restoration Lines and Maintainers of Capsicum annuum L.

“…Plants with the normal or N cytoplasm are fertile and those with the CMS cytoplasm with nuclear restorer genes are fertile, while the plants with the male sterile or S cytoplasm with no restorer genes are male sterile. The restoration of CMS in chili is controlled by a major nuclear gene termed restorer-of-fertility (Rf), along with several modifiers and some environmental factors [11,23].…”