Novel genomic targets for proper subtyping of bovine viral diarrhea virus 1 (BVDV-1) and BVDV-2

Mucellini, Carolina Isabela; Silva Júnior, José Valter Joaquim; de Oliveira, Pablo Sebastian Britto; Weiblen, Rudi; Flores, Eduardo Furtado

doi:10.1007/s11262-023-02022-x

Cited by 2 publications

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References 40 publications

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“…Researchers have designed novel primer sets targeting NS3-NS4A of BVDV-1 (526 bp amplicon) and NS5B of BVDV-2 (728 bp amplicon) for subtyping BVDV. This classification accurately reproduces the subtyping of all the 118 BVDV-1 and 88 BVDV-2 complete/near-complete genomes (CNCGs) from GenBank (Mucellini et al, 2023). BVDV is classified into three genotypes, BVDV-1 (Pestivirus A), BVDV-2 (Pestivirus B), and BVDV-3 (Pestivirus H or HoBi-like virus), in which BVDV-1 currently contains at least 22 subtypes (1a-1v) and BVDV-2 contains at least 4 subtypes (2a-2d) (de Oliveira et al, 2021;Zhu et al, 2022).…”

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confidence: 60%

Diagnosis of bovine viral diarrhea virus: an overview of currently available methods

Wang,

Pang

2024

Front. Microbiol.

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Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea (BVD), which results in significant economic losses in the global cattle industry. Fortunately, various diagnostic methods available for BVDV have been established. They include etiological methods, such as virus isolation (VI); serological methods, such as enzyme-linked immunosorbent assay (ELISA), immunofluorescence assay (IFA), and immunohistochemistry (IHC); molecular methods, such as reverse transcription-polymerase chain reaction (RT-PCR), real-time PCR, digital droplet PCR (ddPCR), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and CRISPR-Cas system; and biosensors. This review summarizes the current diagnostic methods for BVDV, discussing their advantages and disadvantages, and proposes future perspectives for the diagnosis of BVDV, with the intention of providing valuable guidance for effective diagnosis and control of BVD disease.

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confidence: 60%

Diagnosis of bovine viral diarrhea virus: an overview of currently available methods

Wang,

Pang

2024

Front. Microbiol.

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Molecular Characteristics of Bovine Viral Diarrhea Virus Strain Isolated from Commercial Foetal Bovine Serum

Pan,

Jiang,

Duan

et al. 2024

Preprint

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Bovine viral diarrhea virus (BVDV) is commonly detected in biological products such as vaccines and serum. In this study, we have detected BVDV in commercial foetal bovine serum. In order to determine whether the serum contained infectious virus or viral genes, we inoculated the serum sample into MDBK cells. After six passages, results of indirect immunofluorescence assay confirmed that the commercial foetal bovine serum was contaminated with an infectious strain of BVDV, designated as BI-2023. The complete genome sequence of this isolate was 12,273 nt. Subsequent sequence analysis revealed that the 5'UTR genes and the full genome of BI-2023 shared 98% and 94.74% nucleotideidentity, respectively, with the BVDV1b reference strain CC13B. This suggests that BI-2023 represents a new subtype within the BVDV1b lineage. Phylogenetic analysis of the 5'UTR and full genome sequences of BVDVs indicatedthat BI-2023 clusters closely with a known BVDV1blineage. These findings underscore the importance of screening commercial foetal bovine serum for adventitious viruses.

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Novel genomic targets for proper subtyping of bovine viral diarrhea virus 1 (BVDV-1) and BVDV-2

Cited by 2 publications

References 40 publications

Diagnosis of bovine viral diarrhea virus: an overview of currently available methods

Diagnosis of bovine viral diarrhea virus: an overview of currently available methods

Molecular Characteristics of Bovine Viral Diarrhea Virus Strain Isolated from Commercial Foetal Bovine Serum

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