Novel genotype of Ehrlichia canis detected in samples of human blood bank donors in Costa Rica

Bouza-Mora, Laura; Dolz, Gaby; Morales, Álvaro; Romero-Zúñiga, Juan José; Salazar‐Sánchez, Lizbeth; Labruna, Marcelo B.; Aguiar, Daniel Moura de

doi:10.1016/j.ttbdis.2016.09.012

Cited by 72 publications

(71 citation statements)

References 30 publications

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“…18,20,21 Genotypic variations of E. canis has also recently been observed in human blood donors in Costa Rica with serological evidence of zoonotic transmission that share a resemblance to the Brazilian strains. 22 The possibility of distinct genotypes of E. canis causing different clinical manifestation could lead to difficulty in diagnosing canine or human ehrlichiosis. 19,20 Based on the close proximity between domestic dogs and humans, dogs may serve as a sentinel for zoonotic vector-borne diseases in Peru as similarly described in Venezuela.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Tandem Repeat Protein 36 Gene of Ehrlichia canis Detected in Naturally Infected Dogs from Peru

Geiger

Morton

Vasconcelos

et al. 2018

The American Journal of Tropical Medicine and Hygiene

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spp. are emerging infectious pathogens, especially in the Americas. Although is primarily a parasite of dogs, polymerase chain reaction-confirmed human infections have been reported from Mexico, Venezuela, and Costa Rica. This study reports the presence of DNA in 13.7% of 205 dogs from urban areas in Peru and of those, five were analyzed for phylogenetic variation using the Tandem Repeat Protein 36 (TRP36) gene. The use of the TRP36 gene for such analysis was validated against 16S rRNA and heat shock protein genes using Shannon's entropy bioinformatic approach. When compared with other strains previously reported, three unique and novel strains were detected. In addition, the TRP36 amino acid tandem repeat sequences of the Peruvian strains share close similarity to an strain detected from four human blood bank samples in Costa Rica. This study reports for the first time domestic dogs infected with strains closely related to a zoonotic strain, which may be of public health concern as dogs can be chronically infected with this pathogen.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Tandem Repeat Protein 36 Gene of Ehrlichia canis Detected in Naturally Infected Dogs from Peru

Geiger

Morton

Vasconcelos

et al. 2018

The American Journal of Tropical Medicine and Hygiene

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“…Both human granulocytic ehrlichiosis (HGE) and human monocytic ehrlichiosis (HME) caused by E. canis have been reported, albeit in low incidence. Bouza‐Mora et al () reported a 3.6% incidence rate of E. canis from samples of blood donors in Costa Rica. Likewise, another incident of human ehrlichiosis was recently reported in Mexico by Sosa‐Gutierrez et al (), which eventually led to the death of a 32‐year‐old patient, who had previously presented with a history of fever, chills, muscle aches, malaise, headache and loss of appetite for over 10 days.…”

Section: Discussionmentioning

confidence: 99%

“…Perez, Bodor, Zhang, Xiong, and Rikihisa () reported the first case of human ehrlichiosis from six patients in Venezuela, and E. canis was implicated as the causative agent through molecular analysis of their blood samples, as the E. canis strains recovered from their blood samples were genetically identical with those from canine. E. canis is distributed worldwide; hence, the presence of known competent arthropod vectors ( Amblyomma and Rhipicephalus species) and natural reservoir hosts ( Odocoileus virginianus ), to a large extent, determine the distribution of this pathogen (de la Fuente, Estrada‐Pena, Venzal, Kocan, Sonenshine, ; Bouza‐Mora et al, ). This study reported the detection of E. canis genetic material from ticks collected from sheep and goat.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic profiling for zoonoticEhrlichiaspp. from ixodid ticks in the Eastern Cape, South Africa

Adelabu

Iweriebor

Okoh

et al. 2020

Transbound Emerg Dis

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Ticks are obligate hematophagous parasite of vertebrate that transmit a range of pathogenic microorganisms that can cause diseases in livestock and humans. The range of tick-borne disease causative agents infecting domestic animals and humans has recently increased. Several significant zoonotic tick-borne diseases such as ehrlichiosis among others are on the increase worldwide. This study was designed to investigate the occurrence of zoonotic Ehrlichia spp. from samples collected from livestock in selected communities in the Eastern Cape Province, South Africa. Tick samples were manually collected from domesticated animals in selected homesteads.The ticks were morphologically identified to species and tested for Ehrlichia infection via polymerase chain reaction (PCR), using genus-specific disulphide bond formation protein (dsbA) gene primers. This was followed by sequence analysis of amplicons and phylogeny. Of the 1,200 ticks collected, Amblyomma hebraeum was most prevalent (n = 335; 27.9%), followed by Rhipicephalus appendiculatus (n = 274; 22.8%), Rhipicephalus decoloratus; (n = 224; 18.7%) and Rhipicephalus eversti eversti (n = 200, 16.7%). Ehrlichia DNA was detected in 19/1,200 (1.6%) of the screened DNA samples. A homology search of the generated sequences revealed a high percentage of identity between 95% and 98% with other homologous dsbA gene sequences of other Ehrlichia species in GenBank. Phylogenetic analysis showed that the obtained sequences clustered unambiguously with other Ehrlichia sequences from different geographical regions of the world. We concluded that Ehrlichial pathogens are vectored by the ticks collected from domesticated animals in the study areas, thus suggesting concern for public health, as some of the recovered pathogens are zoonotic in nature and could pose serious public health risk through human exposure to tick bites.

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“…La EMC es una enfermedad infecciosa causada por la bacteria Ehrlichia canis, considerada zoonótica (16,17) y emergente, transmitida principalmente por la garrapata del perro (Riphicephalus sanguineus) (18). La etiología de la EMC surge a raíz de la infección por bacterias Gram-negativas del género Ehrlichia (aunque las tinciones con gram no suelen ser útiles) clasificadas dentro del orden de los Rickettsiales y la familia Anaplasmataceae (15).…”

Section: E H R L I C H I a C A N I S Y E F E C T O D E Doxiciclina Enunclassified

“…CME is an infectious disease caused by the Ehrlichia canis bacterium, which is considered to be zoonotic (16,17) and emergent, transmitted mainly by the dog tick (Riphicephalus sanguineus) (18) phagocytophilum) en ejemplares caninos y de igual manera su detección en los vectores conocidos (19)(20)(21).…”

Section: Ehrlichia Canis and The Effects Of Doxycycline In The Treatmentmentioning

confidence: 99%

Farmacocinética y efectos adversos de la doxiciclina en el tratamiento de la Ehrlichiosis: fundamentos teóricos para ensayos clínicos en caninos

Molina

et al. 2017

Rev MVZ Córdoba

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Ehrlichia canis is the causative bacterium of canine monocytic ehrlichiosis (CME), a disease of global importance in veterinary and human medicine. Several studies have addressed the therapeutic efficacy of tetracycline hydrochloride and doxycycline hyclate (HD) for the treatment of CME, however the results are still controversial. Doxycycline is the treatment of choice for canine monocytic ehrlichiosis (CME), a well characterized disease that can serve as a model for research in diseases of the Rickettsial order and tick-borne zoonoses. Although the pharmacokinetics and efficacy of the treatment in the acute and subclinical CME phases have been known for decades, some results also indicate that Ehrlichia canis may persist in clinically normal dogs, even after an extensive treatment regimen. The purpose of this review is to (major delve into/further investigate) the pharmacokinetics and side effects of doxycycline in the treatment of canine ehrlichiosis.Keywords: Blood chemistry, Ehrlichia canis, hematology, pharmacokinetics, tetracycline (Source: CAB). RESUMENEhrlichia canis es la bacteria causante de la Ehrlichiosis monocítica canina (EMC), una enfermedad de importancia global en la medicina veterinaria y humana. Varios estudios han abordado la eficacia terapéutica de hidrocloruro de tetraciclina e hiclato de doxiciclina (HD) para el tratamiento de la EMC, sin embargo los resultados obtenidos siguen siendo controvertidos. La doxiciclina es el tratamiento de elección para la EMC, enfermedad bien caracterizada que puede servir como modelo para las investigaciones en el estudio de las enfermedades del orden de los Rickettsiales y las zoonosis transmitidas por garrapatas. Aunque desde décadas atrás se conoce su farmacocinética y la eficacia en el tratamiento de la EMC en fase aguda y subclínica, algunos resultados también indican que Ehrlichia canis puede persistir en perros clínicamente normales, incluso después de un amplio régimen de tratamiento. El propósito de esta revisión es hacer un acercamiento referente a la farmacocinética y los efectos adversos de la doxiciclina en el tratamiento de la EMC.

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Novel genotype of Ehrlichia canis detected in samples of human blood bank donors in Costa Rica

Cited by 72 publications

References 30 publications

Molecular Characterization of Tandem Repeat Protein 36 Gene of Ehrlichia canis Detected in Naturally Infected Dogs from Peru

Molecular Characterization of Tandem Repeat Protein 36 Gene of Ehrlichia canis Detected in Naturally Infected Dogs from Peru

Phylogenetic profiling for zoonoticEhrlichiaspp. from ixodid ticks in the Eastern Cape, South Africa

Farmacocinética y efectos adversos de la doxiciclina en el tratamiento de la Ehrlichiosis: fundamentos teóricos para ensayos clínicos en caninos

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