Novel grafted electrochemical interface for covalent glucose oxidase immobilization using reactive pentafluorophenyl methacrylate

Analytical Parameters of a Novel Glucose Biosensor Based on Grafted PFM as a Covalent Immobilization Technique

“…Glucose biosensors were prepared by covalent immobilization of GOx using PFM as an enzyme anchor via amide bond formation [ 40 , 41 , 42 , 43 ]. It was demonstrated in previous work that when PFM plasma was grafted onto an organic film of plasma polymerized HEMA-co-EGDA, the immobilized GOx molecules presented conformational active structures [ 38 ]. In previous work, the chemical reaction between PFM groups (plasma polymerized and grafted) was extensively studied, demonstrating the ability of PFM to react with amines, using IRRAS spectroscopy [ 44 ].…”

“…In the present work, it was assumed that the working potential was not affected by the PFM plasma grafting. This modification technique generates isolated brushes of PFM in the electrochemical interface (TiO 2 NTAs/Ti), which should not alter the electrochemical capacity of the biosensor following the reduction of H 2 O 2 to H 2 O [ 38 ].…”

“…The linear range, limit of detection (LOD), and limit of quantification (LOQ) of the Ti/TiO 2 NTAs/ppHEMA-co-EGDA/pgPFM/GOx/Chitosan biosensor were reported in a previous work [ 38 ]. The obtained values were: linear range from 0.25 to 1.49 mM, sensitivity 9.76 µA·mM −1 , LOD 0.10 mM, and LOQ 0.20 mM.…”

“…Then, the PFM plasma grafted surface was performed using an initial film deposition of HEMA-co-EGDA. This process is described in more detail in a previous work [ 38 ]. Briefly, plasma modification techniques were applied to the Ti/TiO 2 NTAs electrode to obtain a polymerized film of HEMA-co-EGDA and a grafted deposition of PFM.…”

“…HPLC measurements were performed using the procedure described by the AOAC Official Method [ 39 ]. Samples for HPLC measurements were prepared as described in our previous work [ 37 , 38 ].…”