Novel histological repertoire of crypt-associated anomalies in inflamed colon mucosa

Rubio, Carlos A.; Lang-Schwarz, Corinna

doi:10.1136/jclinpath-2022-208152

Cited by 9 publications

(4 citation statements)

References 25 publications

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“…Specific histologic features were also assessed as markers of disease or as predictors of response to therapy among IBD patients. For example, extensive crypt eosinophilia and intracryptal epithelial budding can differentiate UC from infectious colitis (15), and the proportion of plasma cells in the lamina propria was found as a robust pretreatment biomarker of response to anti‐tumor necrosis factor‐α therapy (16).…”

Section: Discussionmentioning

confidence: 99%

Correlation Between the Nancy Histopathology Index and Markers of Disease Activity in Pediatric Ulcerative Colitis

Faingelernt

Morgenstern

Matar

et al. 2023

Journal of Pediatric Gastroenterology &Amp; Nutrition

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The Nancy Histological Index (NHI) was developed to assess histological disease activity in adult ulcerative colitis (UC) patients. However, data in pediatrics is limited. Our aim was to determine whether the NHI correlates with different indices of disease activity in pediatric UC patients. We retrospectively reviewed the NHI in rectal biopsies from 61 pediatric UC patients (median age 14.3 years), of whom 34 (55.7%) were newly diagnosed. The median Pediatric Ulcerative Colitis Activity Index (PUCAI) score among participants was 30 (interquartile range 5–55). Most patients exhibited an NHI of 3 (41/61, 67.2%) or 4 (8/61, 13.1%), reflecting moderate-severe histologic inflammation. A moderate positive correlation was identified between the NHI and PUCAI, fecal calprotectin, and Mayo endoscopic scores (r = 0.60, 0.54, and 0.56 respectively, P ≤ 0.001), but not with CRP or albumin. These results indicate that the NHI has a modest correlation with clinical, laboratory and endoscopic indices of disease activity in pediatric UC patients.

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Section: Discussionmentioning

confidence: 99%

Correlation Between the Nancy Histopathology Index and Markers of Disease Activity in Pediatric Ulcerative Colitis

Faingelernt

Morgenstern

Matar

et al. 2023

Journal of Pediatric Gastroenterology &Amp; Nutrition

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“…Recently, the same group demonstrated that specimen orientation could represent a bias in crypt morphology evaluation; a significant portion of the histologic section that resulted in a diagnosis of IBD held cross-cut crypts, an inappropriate sectioning plane that modifies the correct assessment of the other histological parameters that defined the diagnosis of IBD [19][20][21]. On the other side, a series of cryptassociated anomalies were described in addition to crypt branching in crypt destructive inflammations that should be considered in their assessment on histologic slides [22]. These new acquisitions could contribute to the construction of more objective criteria for the differential diagnosis between crypt destructive and nondestructive colitis.…”

Section: Discussionmentioning

confidence: 99%

The Histology-Driven Differential Diagnosis in Bowel Inflammatory Conditions Is Not All That Obvious: Evidence from a Survey Based on Digital Slides

Canavese,

Falco,

Perez-Diaz-del-Campo

et al. 2023

Diagnostics

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(1) Background: when the pathologist faces histologic slides from colonoscopies in daily practice, given the large number of entities and etiologies under inflammatory bowel conditions, in-depth definition of the histological spectrum and the recommendations of current guidelines are often not enough to conclusively define a diagnostic framework. Histological patterns should be organized hierarchically in flowcharts that consider the correlation with clinical data. We conducted an online survey asking a group of gastroenteropathologists to apply a pattern classification based on the most significant lesions in colitis differential diagnosis: crypt distortion and activity. (2) Methods: digital slides from 20 endoscopy samples were analyzed by twenty pathologists and classified according to the occurrence of crypt distortion (nondestructive–destructive colitis) and subsequently to the evidence of activity (ND1-2-3, D1-2). (3) Results: in 8 out of 20 (40%) cases, the participants reached a full agreement regarding the evaluation of crypt distortion (5 cases: nondestructive colitis; 3 cases: destructive colitis). The calculated agreement was k = 0.432. In the second-level quiz (ND1-2-3 and D1-2), full agreement between participants was achieved for 7 of the 28 (25%) possible classifications, with k = 0.229. (4) Conclusions: The findings from this survey are indicative of an unexpectedly low consensus, even among dedicated pathologists, about the recognition of histological changes that are commonly considered critical lesions in the histologic identification of bowel non-neoplastic diseases. In our opinion, these divergences imply a significant risk of misdiagnosis of bowel inflammatory conditions, hampering the usefulness of histological assessment.

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“…Це дає можливість експериментально моделювати специфічні ураження травної системи та екстрапо-Актуальні проблеми сучасної медицини лювати результати на людину [10]. Важливим критерієм оцінки функцій кишкового травлення і всмоктування є глибина крипт слизової оболонки кишечника [11], кількість крипт, їх структура та інтенсивність клітинної інфільтрації [12] .…”

Section: вступunclassified

Morphometrical Indices of Cecum Crypts in Rats Under Influence of Food Additive Complex

Kinash¹,

Hryhorenko²,

Donets³

2023

Act. Probl. of the Modern Med.

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Due to the widespread use of various food additives, concerns about their potential harm to the human health and their safety of approved daily intake levels in food products are increasing. One area of debate is the impact of food additives on the gastrointestinal mucous membrane. Experimental evidence has shown that the consumption of certain food additives, even within acceptable doses, can exacerbate inflammatory processes and contribute to carcinogenesis in individuals with inflammatory bowel diseases. Evaluating the functions of intestinal digestion and absorption requires considering criteria such as the number of crypts in the intestinal mucosa, their depth, structure, and the degree of cellular infiltration. Previous studies have established that the type of food and the presence of certain substances in the diet can affect the depth of crypts, the proliferative activity of crypt cells, and the number of goblet cells in the large intestine. In this study, the dynamic changes in the cecum crypt metrics of rats were analyzed over a period of 16 weeks under the influence of a complex food additive including monosodium glutamate, sodium nitrite, and ponceau 4R. The rats of the experimental group, with access to water ad libitum, were administered with 0,6 mg/kg sodium nitrite, 20 mg/kg monosodium glutamate and 5 mg/kg ponceau 4R in 0.5 ml of distilled water once daily orally. The control group of rats (n=14) had access to drinking water without any additives and received oral saline administration. Collection of samples for histological examination was carried out at 1, 4, 8, 12 and 16 weeks. The morphometric parameters analyzed included the number of crypts in the field of view, their depth, outer diameter, lumen diameter, and the height of epitheliocytes. The results showed that the morphometric parameters of the cecum crypts in rats changed depending on the duration of exposure to the food additive complex of monosodium glutamate, sodium nitrite, and ponceau 4R. The changes observed in the crypt structure and number indicated a significant detrimental effect of the food additive complex on the cecum mucous membrane in rats during the early stages of the experiment. There was an incomplete recovery of the cecum crypts number and structures in the later stages of the experiment; however it occurred in the context of destructive and inflammatory changes in the mucous membrane.

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Novel histological repertoire of crypt-associated anomalies in inflamed colon mucosa

Cited by 9 publications

References 25 publications

Correlation Between the Nancy Histopathology Index and Markers of Disease Activity in Pediatric Ulcerative Colitis

Correlation Between the Nancy Histopathology Index and Markers of Disease Activity in Pediatric Ulcerative Colitis

The Histology-Driven Differential Diagnosis in Bowel Inflammatory Conditions Is Not All That Obvious: Evidence from a Survey Based on Digital Slides

Morphometrical Indices of Cecum Crypts in Rats Under Influence of Food Additive Complex

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