Novel human G protein‐coupled receptors with long N‐terminals containing GPS domains and Ser/Thr‐rich regions

Fredriksson, Robert; Lagerström, Malin C.; Höglund, Pär J.; Schiöth, Helgi B.

doi:10.1016/s0014-5793(02)03574-3

Cited by 82 publications

(72 citation statements)

References 22 publications

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“…Both methods have been successful in ¢nding new genes encoding adhesion GPCRs [13,14]. The HMMs were constructed through alignment of the TM regions of 262 human rhodopsin-like GPCRs [6].…”

Section: Resultsmentioning

confidence: 99%

Seven evolutionarily conserved human rhodopsin G protein‐coupled receptors lacking close relatives

et al. 2003

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We report seven new members of the superfamily of human G protein-coupled receptors (GPCRs) found by searches in the human genome databases, termed GPR100, GPR119, GPR120, GPR135, GPR136, GPR141, and GPR142. We also report 16 orthologues of these receptors in mouse, rat, fugu (pu¡er¢sh) and zebra¢sh. Phylogenetic analysis shows that these are additional members of the family of rhodopsintype GPCRs. GPR100 shows similarity with the orphan receptor SALPR. Remarkably, the other receptors do not have any close relative among other known human rhodopsin-like GPCRs. Most of these orphan receptors are highly conserved through several vertebrate species and are present in single copies. Analysis of expressed sequence tag (EST) sequences indicated individual expression patterns, such as for GPR135, which was found in a wide variety of tissues including eye, brain, cervix, stomach and testis. Several ESTs for GPR141 were found in marrow and cancer cells, while the other receptors seem to have more restricted expression patterns.

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Section: Resultsmentioning

confidence: 99%

Seven evolutionarily conserved human rhodopsin G protein‐coupled receptors lacking close relatives

et al. 2003

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“…[31][32][33]. It is difficult to know exactly why these genes have not previously been identified, but this could probably be related to the usage of different gene finding programs to generate ab initio sets as well as the different thresholds used for identifying EST evidence for the predicted genes.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the current RefSeq, Ensembl and EST databases for counting genes and gene discovery

et al. 2004

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Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human genes can be found. The results indicate that there are about 25 000 unique genes in the union of RefSeq and Ensembl with 12-18% and 8-13% of the genes in each set unique to the other set, respectively. About 20% of all genes had splice variants. There are a considerable number of ESTs (2 200 000) that do not match the identified genes and we used an in-house pipeline to identify 22 novel genes from Genscan predictions that have considerable EST coverage. The study provides an insight into the current status of human gene catalogues and shows that considerable refinement of methods and datasets is needed to come to a conclusive gene count.

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“…AY140958). They have been classified as novel aGPCR in human and encode putative aGPCR in the mouse genome (Fredriksson et al, 2002;Bjarnadottir et al, 2007;Haitina et al, 2008;Lum et al, 2010). Gpr116 was initially named Ig-Hepta because of its two immunoglobulin and 7TM spanning domains (Abe et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Characterization and functional study of a cluster of four highly conserved orphan adhesion‐GPCR in mouse

Prömel

Waller-Evans

Dixon

et al. 2012

Developmental Dynamics

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Background: Adhesion G protein-coupled receptors (aGPCR) constitute a structurally and functionally diverse class of seven-transmembrane receptor proteins. Although for some of the members important roles in immunology, neurology, as well as developmental biology have been suggested, most receptors have been poorly characterized. Results: We have studied evolution, expression, and function of an entire receptor group containing four uncharacterized aGPCR: Gpr110, Gpr111, Gpr115, and Gpr116. We show that the genomic loci of these four receptors are clustered tightly together in mouse and human genomes and that this cluster likely derives from a single common ancestor gene. Using transcriptional profiling on wild-type and knockout/LacZ reporter knockin mice strains, we have obtained detailed expression maps that show ubiquitous expression of Gpr116, co-expression of Gpr111 and Gpr115 in developing skin, and expression of Gpr110 in adult kidney. Loss of Gpr110, Gpr111, or Gpr115 function did not result in detectable defects, indicating that genes of this aGPCR group might function redundantly. Conclusions: The aGPCR cluster Gpr110, Gpr111, Gpr115, and Gpr116 developed from one common ancestor in vertebrates. Expression suggests a role in epithelia, and one can speculate about a possible redundant function of GPR111 and GPR115. Developmental Dynamics 241:1591-1602, 2012.V C 2012 Wiley Periodicals, Inc.

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Novel human G protein‐coupled receptors with long N‐terminals containing GPS domains and Ser/Thr‐rich regions

Cited by 82 publications

References 22 publications

Seven evolutionarily conserved human rhodopsin G protein‐coupled receptors lacking close relatives

Seven evolutionarily conserved human rhodopsin G protein‐coupled receptors lacking close relatives

Comparison of the current RefSeq, Ensembl and EST databases for counting genes and gene discovery

Characterization and functional study of a cluster of four highly conserved orphan adhesion‐GPCR in mouse

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