Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers

Abstract

SYNOPSISHydrophilic nanoparticulate carriers have important potential applications for the administration of therapeutic molecules. The recently developed hydrophobic-hydrophilic carriers require the use of organic solvents for their preparation and have a limited protein-loading capacity. To address these limitations a new approach for the preparation of nanoparticles made solely of hydrophilic polymers is presented. The preparation technique, based on an ionic gelation process, is extremely mild and involves the mixture of two aqueous phases at room temperature. One phase contains the polysaccharide chitosan (CS) and a diblock copolymer of ethylene oxide and propylene oxide (PEO-PPO) and, the other, contains the polyanion sodium tripolyphosphate (TPP). Size (200-1000 nm) and zeta potential (between /20 mV and /60 mV) of nanoparticles can be conveniently modulated by varying the ratio CS/PEO-PPO. Furthermore, using bovine serum albumin (BSA) as a model protein it was shown that these new nanoparticles have a great protein loading capacity (entrapment efficiency up to 80% of the protein) and provide a continuous release of the entrapped protein for up to 1 week.

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

“…As can be observed in Figure 2, pullulan-based nanoparticles evidence similar structure and morphology, corresponding to spherical and compact nanoparticles. This morphology corresponds to that widely reported for many formulations of polysaccharide-based nanoparticles (Calvo et al, 1997b;Goycoolea et al, 2009;Oyarzun-Ampuero et al, 2009;Sarmento et al, 2006;TeijeiroOsorio et al, 2009;Zorzi et al, 2011).…”

Chelators influenced synthesis of chitosan–carboxymethyl cellulose microparticles for controlled drug delivery

“…0.2, 0.4 and 0.6 % TPP were prepared in 50 ml deionized water (Calvo et al 1997). TPP solution was added dropwise to CS solution at room temperature.…”

Chitosan-based nanogels for selective delivery of photosensitizers to macrophages and improved retention in and therapy of articular joints

“…Several strategies involving nanosized carriers for photosensitizer delivery such as liposomes, polymeric micelles, metal-based or silica-based nanoparticles have already been explored [15][16][17][18]. The preparation of chitosan-based hydrophilic nanogels has been widely described in the literature, employing penta sodium triphosphate (PSTP) as the complexing anionic molecule for the polycation chitosan [19][20][21][22][23][24][25]. Chitosan-based nanogels have been studied as carriers of oligonucleotides in gene therapy, for mucosal vaccination, in tissue engineering or drug delivery [26][27][28].…”