Novel ‘CandidatusLiberibacter’ species identified in the Australian eggplant psyllid,Acizzia solanicola

Morris, Jacqueline M; Shiller, Jason; Mann, Rachel; Smith, Grant R.; Yen, Alan L.; Rodoni, Brendan

doi:10.1111/1751-7915.12707

Cited by 47 publications

(31 citation statements)

References 49 publications

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“…Liberibacter brunswickensis’ (Lbr) are not known to be associated with plant disease (Raddadi et al , ; Leonard et al , ). Lbr was first identified in the Australian eggplant psyllid, Acizzia solanicola (Morris et al , ). Lcr was first isolated from the phloem sap of a defoliated Babaco papaya, Carica stipulata × Carica pubescens , in Puerto Rico in 1995 (Leonard et al , ).…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide analyses of Liberibacter species provides insights into evolution, phylogenetic relationships, and virulence factors

Thapa

Francesco

Trinh

et al. 2020

Molecular Plant Pathology

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Candidatus Liberibacter' species are insect-transmitted, phloem-limited α-Proteobacteria in the order of Rhizobiales. The citrus industry is facing significant challenges due to huanglongbing, associated with infection from 'Candidatus Liberibacter asiaticus' (Las). In order to gain greater insight into 'Ca. Liberibacter' biology and genetic diversity, we have performed genome sequencing and comparative analyses of diverse 'Ca. Liberibacter' species, including those that can infect citrus. Our phylogenetic analysis differentiates 'Ca. Liberibacter' species and Rhizobiales in separate clades and suggests stepwise evolution from a common ancestor splitting first into nonpathogenic Liberibacter crescens followed by diversification of pathogenic 'Ca. Liberibacter' species. Further analysis of Las genomes from different geographical locations revealed diversity among isolates from the United States. Our phylogenetic study also indicates multiple Las introduction events in California and spread of the pathogen from Florida to Texas. Texan Las isolates were closely related, while Florida and Asian isolates exhibited the most genetic variation. We have identified conserved Sec translocon (SEC)-dependent effectors likely involved in bacterial survival and virulence of Las and analysed their expression in their plant host (citrus) and insect vector (Diaphorina citri). Individual SEC-dependent effectors exhibited differential expression patterns between host and vector, indicating that Las uses its effector repertoire to differentially modulate diverse organisms. Collectively, this work provides insights into the evolution of 'Ca. Liberibacter' species, the introduction of Las in the United States and identifies promising Las targets for disease management. K E Y W O R D S 'Candidatus Liberibacter' sp., citrus greening disease, HLB, huanglongbing, phylogenomics, SEC effector | 717 THAPA eT Al.

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Section: Introductionmentioning

confidence: 99%

Genome‐wide analyses of Liberibacter species provides insights into evolution, phylogenetic relationships, and virulence factors

Thapa

Francesco

Trinh

et al. 2020

Molecular Plant Pathology

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“…The citrus industry is on the precipice of obliteration. A successful, sustainable treatment method for HLB remains elusive; yet, new Liberibacter species and subspecies continues to emerge [Morris et al, 2017;Roberts et al, 2015;Roberts and Pietersen, 2017;Wu et al, 2015;Zheng et al, 2017]. A variety of important crops are infected by this bacteria genus, the Solanaceae family is probably the most representative and economically significant [Hawkes, 1999].…”

Section: Discussionmentioning

confidence: 99%

Understanding the Physiology of Liberibacter asiaticus: An Overview of the Demonstrated Molecular Mechanisms

Coyle¹,

Lorca²,

González³

2018

Microb Physiol

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Citrus greening disease, or huanglongbing, may entirely eradicate all varieties of citrus cultivars worldwide in the near future. This disease is caused by non-cultivable bacteria of the genus Liberibacter; among them, the more pathogenic being Liberibacter asiaticus. The complexity of the host–pathogen relationship, associated with the impossibility of performing research using axenic cultures, has severely hindered the basic research on microbiology. Since its genome sequence was published in 2009, most of the scientific publications in the field were dedicated to in silico analysis and selection of targets to design early detection methods. The knowledge gained with these approaches felt short to articulate effective methods to control the disease progression. There is a critical need to understand the basic biology of bacteria to design effective strategies to inactivate central mechanisms of pathogenesis. In this review, we summarize the scientific progress made by studying L. asiaticus’ biology through direct experimentation. The evidence collected thus far is not enough to understand L. asiaticus’ fundamental biology. It is imperiously necessary to increase the basic research to identify relevant biological clues to control citrus greening. The gained knowledge may also help to prevent potential catastrophic diseases in other crops of significant importance caused by other unculturable Liberibacter species.

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“…L. europaeus (Leu) infecting scotch broom (Thompson et al 2013), L. crescens (Lcr), and Ca. L. brunswickensis (Lbr) (Hansen et al 2008;Jagoueix et al 1994Jagoueix et al , 1997Leonard et al 2012;Liefting et al 2008;Morris et al 2017;Raddadi et al 2011;Texeira et al 2005;Thompson et al 2013). One potential explanation of the evolutionary pathway of Liberibacters is "plant-first model".…”

Section: Potential Evolutionary Pathways Of Liberibactersmentioning

confidence: 99%

A perspective of citrus Huanglongbing in the context of the Mediterranean Basin

Wang

2020

J Plant Pathol

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Huanglongbing (HLB) is one of the most catastrophic citrus diseases. HLB pathogens Candidatus Liberibacter asiaticus (Las), Ca. L. africanus (Laf), and Ca. L. americanus (Lam) and their insect vectors Asian citrus psyllids (ACP, Diaphorina citri) and African citrus psyllids (AfCP, Trioza erytreae) are invading citrus producing regions where HLB was absent previously, including the Mediterranean basin. Importantly, the Mediterranean region is one of the two major citrus producing areas without HLB. Here, I provide a short perspective regarding a) information related to the distribution of the HLB pathogens and psyllid vectors in this region and neighboring countries, b) predicted distribution of the HLB for this region, c) the possible evolution of Liberibacters and how they could have established their relationship with different hosts, and d) approaches to fend off HLB. Specifically, I emphasized the following measurements: quarantine measures against AfCP, ACP, Las, Laf, and Lam, early detection and diagnosis of HLB and removal of inoculum, surveillance of AfCP and ACP and eradication of citrus psyllids once identified. Keywords Citrus. HLB. Huanglongbing. Liberibacter. Phloem. Psyllids Citrus Huanglongbing (HLB), also known as citrus greening, is the most devastating disease in many citrus producing areas worldwide. HLB is caused by Candidatus Liberibacter asiaticus (Las), Ca. L. africanus (Laf), and Ca. L. americanus (Lam) that are yet to be cultured (Merfa et al. 2019). Among them, Las is the most widely distributed and has been reported in Asia, North America, South America, and Africa, Laf is only present in Africa, and Lam, originally identified in Brazil, is diminishing and being taken over by Las. Las and Lam are naturally transmitted by Asian citrus psyllids (ACP, Diaphorina citri) and Laf by African citrus psyllids (AfCP, Trioza erytreae). The HLB pathogens and insect vectors have been spreading to new territories including Brazil (Coletta-Filho et al. 2004; Texeira et al. 2005), multiple states of the United States (Kumagai et al. 2013); Belize (Manjunath et al. 2010); countries in the Caribbean including Cuba (Martinez et al. 2009) and the Dominican Republic (Matos et al. 2009);

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Novel ‘CandidatusLiberibacter’ species identified in the Australian eggplant psyllid,Acizzia solanicola

Cited by 47 publications

References 49 publications

Genome‐wide analyses of Liberibacter species provides insights into evolution, phylogenetic relationships, and virulence factors

Genome‐wide analyses of Liberibacter species provides insights into evolution, phylogenetic relationships, and virulence factors

Understanding the Physiology of <b><i>Liberibacter asiaticus</i></b>: An Overview of the Demonstrated Molecular Mechanisms

A perspective of citrus Huanglongbing in the context of the Mediterranean Basin

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