Novel identification of biofluids using a multiplex methylation sensitive restriction enzyme-PCR system

Lin, Yu-Chih; Tsai, Li-Chin; Lee, James Chun-I; Su, Chung‐Kuang; Tzen, Jason T. C.; Linacre, Adrian; Hsieh, Hsing-Mei

doi:10.1016/j.fsigen.2016.08.011

Cited by 19 publications

(22 citation statements)

References 42 publications

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“…We have also confirmed the usefulness of the STATH and HTN3 genes [40,41] and performed various studies to introduce the method into practice [42][43][44]. In addition, methods focusing on microRNA [45][46][47][48][49][50] and DNA methylation [51][52][53][54][55][56] have been actively explored for saliva identification. At present, it would seem that further studies are necessary before microRNA and DNA methylation methods can be applied to practical work.…”

Section: Saliva Identificationmentioning

confidence: 54%

“…Furthermore, methods using Raman spectroscopy [111] or Fourier-transform infrared spectroscopy [112] have been reported as non-destructive methods for analysis that do not even consume a minute amount of residual sample, although further studies are required before they can be practically applied. [37][38][39][40]45,46,[48][49][50][51][52][53][54][55][56]76,77] Vaginal fluid Immunodiffusion Immunoelectrophoresis [34] Papanicolaou staining Lugol's staining [79][80][81][82] mRNA microRNA DNA methylation [35,38,41,45,46,48,49,[52][53][54][55][90][91][92][93] Vaginal bacteria [83][84][85][86][87][88][89] GC-MS LC-MS [94,…”

Section: Discussionmentioning

confidence: 99%

“…As PRM2 is expressed in the sperm itself, it will not be expressed in the semen of azoospermic individuals. In addition, as for saliva, there are many reports on the use of microRNA [45,46,[48][49][50] and DNA methylation [51][52][53][54][55][56]76,77] in semen identification.…”

Section: Semen Identificationmentioning

confidence: 99%

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Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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Although, DNA typing plays a decisive role in the identification of persons from blood and body fluid stains in criminal investigations, clarifying the origin of extracted DNA has also been considered an essential task in proving a criminal act. This review introduces the importance of developing precise methods for body fluid identification. Body fluid identification has long relied on enzymatic methods as a presumptive assay and histological or serological methods as a confirmatory assay. However, because the latest DNA typing methods can rapidly obtain results from very small and even old, poorly preserved samples, the development of a novel corresponding body fluid identification method is required. In particular, an immunochromatographic method has been introduced to identify saliva and semen from sexual crimes. In addition, for vaginal fluid identification, attempts have been made in the past decade to introduce a method relying on body fluid-specific mRNA expression levels. At present, the development of molecular biological methods involving microRNA, DNA methylation, and resident bacterial DNA is ongoing. Therefore, in criminal investigations, body fluid identification is an essential task for correctly applying the results of DNA typing, although further research and development are required.

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Section: Saliva Identificationmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

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Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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“…Several examples of DNA methylation mark-ers analysis for cell and tissue type identification include validation of a set of 9 DNA methylation markers for the identification of blood, saliva, semen, vaginal fluid, and menstrual blood [28]. Similarly, using the Illumina 450K database, Lin et al [29] identified a set of 8 tissue-specific epigenetic markers. In addition to that, Vidaki et al [30] identified 2 novel semen-specific CpG markers (cg04382920 and cg11768416) for epigenetic forensics of samples derived from sexual assaults.…”

Section: Sample Source Cell/tissue Typementioning

confidence: 99%

Forensic Epigenetic Analysis: The Path Ahead

Sabeeha

Hasnain

2019

Med Princ Pract

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Unlike DNA fingerprinting, which scores for differences in the genome that are phenotype neutral, epigenetic variations are gaining importance in forensic investigations. Methylation of DNA has a broad range of effects on the lifestyle, health status, and physical appearance of individuals. DNA methylation profiling of forensic samples is useful in determination of the cell or tissue type of the DNA source and also for estimation of age. The quality and quantity of the biosample available from the crime scene limits the possible number of DNA methylation tests and the selection of the technology that can be used. Several techniques have been used for DNA methylation analysis for epigenetic investigations of forensic biological samples. However, novel techniques are needed for multiplex analysis of epigenetic markers as the techniques that are currently available require a large amount of high-quality DNA and are also limited in their multiplexing capacities that are often insufficient to fully resolve a forensic query of interest.

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“…The stability of these methylation markers in trace amounts of various body fluids may be marred by both endogenous and exogenous factors, and therefore a study using a large number of samples (100 for each body fluid) were performed using Illumina BeadChip Array, which showed comparable sensitivity of the results to STR analysis (Forat et al, 2016). The methylation pattern differences in the DNA of these various body fluids has been recently utilized to develop another novel forensic technique based on combined use of COBRA and MSP methods (Lin et al, 2016).…”

Section: Sources Of Tissue/body Fluid Identificationmentioning

confidence: 99%

Crime investigation through DNA methylation analysis: methods and applications in forensics

Rana¹

2018

Egypt J Forensic Sci

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Discerning monozygotic twins, precisely predicting the age of human beings and most importantly interpreting human behavior remains a big challenge in forensic science. Modern advances in the field of epigenetics especially analysis of DNA methylation pattern can certainly help to identify and discriminate various single-source DNA samples such as bodily fluids collected from the crime scene. In this review, cytosine methylation analysis of DNA has been methodologically discussed with a broad range of applications in forensic science such as identifying single or mixed body fluid, discriminating monozygotic twins, age prediction, addiction/behavioral prediction as well as race/ethnicity identification. With the advances of next-generation sequencing techniques, blooming of DNA methylation datasets and together with standard epigenetic protocols, the prospect of investigating and solving these critical issues in forensic science is highly promising in the court of law.

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Novel identification of biofluids using a multiplex methylation sensitive restriction enzyme-PCR system

Cited by 19 publications

References 42 publications

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Forensic Epigenetic Analysis: The Path Ahead

Crime investigation through DNA methylation analysis: methods and applications in forensics

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