Novel Identification of LYVE-1 Positive Macrophages in Rheumatoid Synovial Tissue

Rump, Lisa; Mattey, Derek L.; Kehoe, Oksana; Middleton, Jim

doi:10.4236/ojra.2016.63012

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…Lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1) is known as a marker for lymphangiogenesis, which is also a considerable process for disease progression and potentially exacerbates inflammatory cell persistence. It is reported that LYVE-1+ macrophages were present in RA synovium [6]. …”

Section: Introductionmentioning

confidence: 99%

Exosomal amyloid A and lymphatic vessel endothelial hyaluronic acid receptor-1 proteins are associated with disease activity in rheumatoid arthritis

et al. 2017

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BackgroundExosomes are thought to play an important role in exchanging information between cells. The proteins and lipids in exosomes play roles in mediating inflammatory and autoimmune diseases. The aim of this study was to identify exosomal candidate proteins that are related to other inflammatory parameters in rheumatoid arthritis (RA).MethodsThe study population consisted of 60 patients with RA: 30 in the clinical remission (CR) group with a Disease Activity Score in 28 joints based on erythrocyte sedimentation rate (DAS28-ESR) ≤2.6 and 30 in the non-clinical remission (non-CR) group with a DAS28-ESR >2.6. Preparation of exosomes from patient serum samples was performed with the ExoQuick kit, and protein identification/quantification was performed using tandem mass tag labeling/mass spectrometry and an enzyme-linked immunosorbent assay. Comparisons between groups were made using Student’s t test or the Mann-Whitney U test, as appropriate. Spearman’s correlation coefficients (ρ) were calculated.ResultsWe identified six candidate proteins. Exosomal levels of amyloid A (AA) and lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1) differed between the CR and non-CR groups. Both serum and exosomal AA levels were higher in the non-CR group than in the CR group (p = 0.001). Significant positive correlations were found between exosomal AA and C-reactive protein (CRP) as well as between serum AA and CRP (ρ = 0.614, p = 0.001, and ρ = 0.624, p = 0.001, respectively). Although serum levels of LYVE-1 did not differ between the non-CR and CR groups, exosomal levels of LYVE-1 were lower in the non-CR group than in the CR group (p = 0.01). We identified positive correlations between serum/exosomal LYVE-1 and CRP only in the non-CR group (serum ρ = 0.376, p = 0.04; exosome ρ = 0.545, p = 0.002).ConclusionsExosomal LYVE-1 shows potential for use as an additional marker of disease activity in patients with RA, and exosomes may carry other useful markers for RA.

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Section: Introductionmentioning

confidence: 99%

Exosomal amyloid A and lymphatic vessel endothelial hyaluronic acid receptor-1 proteins are associated with disease activity in rheumatoid arthritis

et al. 2017

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Identification of Lyve‐1 positive macrophages as resident cells in meninges of rats

Brezováková

Jadhav

2020

J of Comparative Neurology

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Meningeal immunity along with its associated lymphatic vasculatures is widely discussed recently. Lymphatic vessels in meninges drain interstitial fluid into the deep‐cervical lymph nodes. The vessels are composed of cells that express the cardinal marker for lymphatic endothelium—the lymphatic vessel hyaluronan receptor‐1 (Lyve‐1). However, studies also show the presence of nonendothelial Lyve‐1 expressing cells in certain tissues. Therefore, we were curious if nonendothelial Lyve‐1+ cells are also present in dura mater of meninges. We show that Lyve‐1+ endothelial cells are distributed adjacent to the blood vessels in the brain dura mater of rats. We did not observe any lymphatic vessels in spinal dura mater. Interestingly, we also observed isolated population of nonlymphatic Lyve‐1+ cells in both brain and spinal dura mater. Morphologically, the Lyve‐1+ cells were extensively pleomorphic, sometimes elongated or round. Surprisingly, the thoracolumbal meningeal Lyve‐1+ cells were predominantly round in morphology. Using endothelial specific marker VEGFR3 and macrophage markers CD68 and CD169, we observed that the isolated Lyve‐1+ cells lacked endothelial cell signature, but were either CD68+ or CD169+ macrophages. Moreover, we observed that the Lyve‐1+ cells colocalized with collagen fibers in the meninges, and some of Lyve‐1+ cells had intracellular collagen. The study for the first time demonstrates the presence of Lyve‐1 positive macrophages in the lymphatic and nonlymphatic regions in the meninges of rats.

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Novel Identification of LYVE-1 Positive Macrophages in Rheumatoid Synovial Tissue

Cited by 2 publications

References 16 publications

Exosomal amyloid A and lymphatic vessel endothelial hyaluronic acid receptor-1 proteins are associated with disease activity in rheumatoid arthritis

Exosomal amyloid A and lymphatic vessel endothelial hyaluronic acid receptor-1 proteins are associated with disease activity in rheumatoid arthritis

Identification of Lyve‐1 positive macrophages as resident cells in meninges of rats

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