Novel immunosensor platform based on inorganic barium strontium titanate film for human IgG detection

Fang, Xiaoqin; Tan, Ooi Kiang; Gan, Yik Yuen; Tse, Man Siu

doi:10.1016/j.snb.2010.06.047

Cited by 13 publications

(8 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BSA is also a major component in the FBS ( 70 to 80%). Thus the adsorption of FBS followed the same trend as BSA adsorption 49 , 50 . As the electrical properties (specifically the ) is an intrinsic property related to the charge holding capacity of the material, the relationship between the adsorption and can be interesting to probe.…”

Section: Resultsmentioning

confidence: 65%

See 1 more Smart Citation

Microstructural, electrical and biological activity in $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2-\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_3$$ ceramic composites designed for tissue engineering applications

Das

Pamu

Bhardwaj

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The article investigates electrically active ceramic composite of $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2$$ Ca 10 ( PO 4 ) 6 ( OH ) 2 (HAP) and $$\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_{3}$$ Ba 0.5 Sr 0.5 TiO 3 (BST) for biomedical applications. The study is a systematic blend of the materials science aspect of composites with a special focus on the dielectric and biological properties and their relationships. The article emphasized primarily extracting the dielectric constant ($$\epsilon _r)$$ ϵ r ) of the specimens (that lay in the range of 3–65) and related them to microstructural properties like the grain size and at.% of BST. A broad outlook on the importance of $$\epsilon _r$$ ϵ r in determining the suitability of bioceramics for clinical applications is presented. Bioactivity analysis of the specimens led to probing the surface charges (that were negative), and it was found crucial to the growth of dense apatite layers. Furthermore, the cytocompatibility of the specimens displayed cell viability above 100% for Day 1, which increased substantially for Day 3. To reveal other biological properties of the composites, protein adsorption studies using bovine serum albumin (BSA) and fetal bovine serum (FBS) was carried out. Electrostatic interactions govern the adsorption, and the mathematical dependence on surface charges is linear. The protein adsorption is also linearly correlated with the $$\epsilon _r$$ ϵ r , intrinsic to the biomaterials. We delve deeper into protein–biomaterials interactions by considering the evolution of the secondary structure of BSA adsorbed into the specimens. Based on the investigations, 20 at.% HAP–80 at.% BST (20H–80B) was established as a suitable composite comprising the desired features of HAP and BST. Such explorations of electrical and biological properties are interesting for modulating the behavior of bioceramic composites. The results project the suitability of 20H–80B for designing electrically active smart scaffolds for the proposed biomedical applications and are expected to incite further clinical trials.

show abstract

Section: Resultsmentioning

confidence: 65%

“…BST, on the other hand, consists of , and positively charged ions. Thus the enhanced electrostatic interactions with negatively charged BSA molecules 48 – 50 resulted in a higher adsorbed amount on BSA. The XRD analysis in section Structural analysis reveals the formation of SAP in the case of the composites.…”

Section: Resultsmentioning

confidence: 99%

Microstructural, electrical and biological activity in $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2-\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_3$$ ceramic composites designed for tissue engineering applications

Das

Pamu

Bhardwaj

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Perovskite oxides; MTiO3 titanates (M = Zn, Sr, Fe, Cd, Ba, Pb, etc.) have been regarded as materials having various applications in solid-oxide fuel cells electrodes; SOFCs [1], metalbarriers [2], sensors [3], electronics [4] and catalysts [5]. As a well-known member of this family, Zinc titanate (ZnTiO3) was already used as pigment [6], dye adsorbent [7], sensor for NO and CO gases [8], microwave resonator materials [9], heat reflective pigment [10] and photocatalyst [11].…”

Section: Introductionmentioning

confidence: 99%

Zinc Titanate Nanopowders. Synthesis and Characterization

Gabal

Angari

2021

Preprint

View full text Add to dashboard Cite

Zinc titanates nanopowders viz.; Zn2TiO4, ZnTi3O8 and ZnTiO3 were synthesized through the thermal decomposition course of ZnC2O4.2H2O-TiO2 precursor (1:1 mole ratio), prepared via a new co-precipitation method up to 900oC. Thermogravimetric measurement (TG) was utilized to characterize the precursor decomposition while X-ray diffraction (XRD), Fourier transform infra-red (FT-IR) were used to characterize the decomposition products as well as the phase transitions at different temperatures. XRD revealed the starting of titanates formation at 700oC via detecting Zn2TiO4 along with ZnO and TiO2 (anatase) diffraction peaks. By increasing the calcination temperature to 800oC, the ZnO content vanished with the appearing of Zn2Ti3O8 besides ZnTi2O4 and impurities of TiO2 (anatase). Finally at 900oC, the Zn2Ti3O8 content was decomposed into ZnTiO3. Nitrogen adsorption-desorption isotherm of the calcined precursor at 900oC indicated low specific surface area of 7.1 m2 g-1 in accordance with the agglomeration nature estimated via transmission electron microscopy (TEM) study. The conductivity measurements showed semiconducting behavior of the prepared titanates with ferroelectric transition in the range 200-308oC.The obtained low dielectric value suggests the uses of present titanates as a co-fired ceramic or resonator ceramics.

show abstract

“…Here, the Si-OH and the -NH 2 groups stabilize the nanoparticles while the presence of only one of these functional groups is not enough to complete the task. [38][39][40][41][42][43][44][45][46][47][48][49] Among the various hybrid nanomaterials, CNT-AuNPs have been used widely for immunoassays. In this nanodisperse AuNPs sol solution, puried MWCNT was mixed along with ethanol that reduces the interfacial tension between AuNPs sol solution and MWCNT.…”

Section: Introductionmentioning

confidence: 99%

Controllable gold nanoparticle deposition on carbon nanotubes and their application in immunosensing

et al. 2015

View full text Add to dashboard Cite

A CNT-AuNPs hybrid nanocomposite platform was prepared from nanodisperse AuNPs in N- [3-(trimethoxysilyl)propyl]ethylenediamine (EDAS) sol-gel matrices with purified MWCNT. EDAS, an amine group-containing sol-gel solution, was utilized for its ability to stabilize the nanoparticles in solution. The developed model system was based on immobilized rabbit anti-mouse IgG-HRP (horseradish peroxidase) for reagentless detection of mouse IgG. The immunosensing platform was prepared by using Nafion for the immobilization of rabbit anti-mouse IgG-HRP and CNT-AuNPs hybrid nanocomposite on a glassy carbon electrode used for the detection of mouse IgG which provides a biocompatible microenvironment. The resulting CNT-AuNPs nanocomposite brings new capabilities for electrochemical devices by using the synergistic action of its electrocatalytic activity. The CNT-AuNPs were characterized using SEM, TEM, EIS, and AFM, and the analytical performance was monitored by differential pulse voltammetry. The detection limit of mouse IgG is 0.5 ng mL À1 (S/N ratio ¼ 3). In addition, the immunosensor efficiently allowed a specific electrochemical analysis of mouse IgG and easy discrimination of goat IgG, chicken IgG, and rabbit IgG.

show abstract

Novel immunosensor platform based on inorganic barium strontium titanate film for human IgG detection

Cited by 13 publications

References 29 publications

Microstructural, electrical and biological activity in $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2-\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_3$$ ceramic composites designed for tissue engineering applications

Microstructural, electrical and biological activity in $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2-\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_3$$ ceramic composites designed for tissue engineering applications

Zinc Titanate Nanopowders. Synthesis and Characterization

Controllable gold nanoparticle deposition on carbon nanotubes and their application in immunosensing

Contact Info

Product

Resources

About