Novel in situ biosynthesized fluorescent zinc nanoclusters for specific cellular bio-imaging

Su, Meina; Ye, Jing; Li, Qiwei; Li, Shuihong; Ge, Wei; Chen, Xiao; Jiang, Hui; Wang, Xuemei

doi:10.1016/j.cclet.2015.07.021

Cited by 11 publications

(16 citation statements)

References 15 publications

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“…This implied that only cells which are tumorigenic in nature should be capable of synthesizing P:NCs in situ. In fact, this very concept was the foundation for the specific tumor imaging application proposed in the above studies (2,7,8,19).…”

Section: Introductionmentioning

confidence: 97%

“…The first in situ synthesis of green fluorescent P:AuNCs was demonstrated in tumorigenic cells by Wang et al in 2013 (7). Very recently, the in 4 situ synthesis of protein stabilized silver, zinc, and platinum nanoclusters in tumorigenic cells have also been published (2,8,19). Interestingly, the synthesis of near IR emitting AgNCs was non-spontaneous and necessitated the development of new protocols requiring additional steps for stable cluster formation (8).…”

Section: Introductionmentioning

confidence: 97%

“…Interestingly, the synthesis of near IR emitting AgNCs was non-spontaneous and necessitated the development of new protocols requiring additional steps for stable cluster formation (8). These authors describe a preliminary mechanism in which the reduction of Au(III), Ag (II), Pt(IV), or Zn(II) leading to the formation of AuNCs, AgNCs, ZnNCs, and PtNCs in situ could be attributed to the high levels of either the reactive oxygen and nitrogen species (ROS and RNS) present in tumorigenic cells, or the elevated levels of intracellular reductants such as GSH-GSSG and NAD(P)H that work to mitigate ROS and RNS in tumorigenic cells (2,7,8,19,20). In fact, H 2 O 2 has been demonstrated as a reducing agent for gold nanoparticle synthesis (21)(22)(23)(24)(25), as has glutamate, a building block of GSH (26)(27)(28).…”

Section: Introductionmentioning

confidence: 98%

“…Protein stabilized noble metal nanoclusters (P:NC) present an exciting option as an intracellular fluorescent marker (1)(2)(3)(4)(5)(6)(7)(8). P:NCs are small clusters of atoms ranging from 5 to 25 atoms which are comprised of an Au 0 core surrounded by a shell of Au + atoms that are bound to the stabilizing protein (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In situSynthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

West¹,

Schaeublin

Griep³

et al. 2016

ACS Appl. Mater. Interfaces

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To date, the directed in situ synthesis of fluorescent gold nanoclusters (AuNCs) has only been demonstrated in cancerous cells, with the theorized synthesis mechanism prohibiting AuNC formation in nontumorigenic cell lines. This limitation hinders potential biostabilized AuNC-based technology in healthy cells involving both chemical and mechanical analysis, such as the direct sensing of protein function and the elucidation of local mechanical environments. Thus, new synthesis strategies are required to expand the application space of AuNCs beyond cancer-focused cellular studies. In this contribution, we have developed the methodology and demonstrated the direct in situ synthesis of AuNCs in the nontumorigenic neuronal microglial line, C8B4. The as-synthesized AuNCs form in situ and are stabilized by cellular proteins. The clusters exhibit bright green fluorescence and demonstrate low (<10%) toxicity. Interestingly, elevated ROS levels were not required for the in situ formation of AuNCs, although intracellular reductants such as glutamate were required for the synthesis of AuNCs in C8B4 cells. To our knowledge, this is the first-ever demonstration of AuNC synthesis in nontumorigenic cells and, as such, it considerably expands the application space of biostabilized fluorescent AuNCs.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In situSynthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

West¹,

Schaeublin

Griep³

et al. 2016

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…It is well known that the deep‐seated tumors can only be diagnosed at the terminal stages, i.e., after metastasis to other vital organs. Medical imaging technology is developing rapidly and has been applied in various fields, such as magnetic resonance imaging (MRI), nuclide imaging (Positron Emission Computed Tomography (PET)), ultrasound imaging, PET‐ computed tomography (CT) imaging, CT imaging, optical imaging, and fluorescence bioimaging . Optical or fluorescence imaging, along with advanced fluorescent biomarkers play a pivotal role in exploring the fundamentals of cellular activities and real‐time monitoring of the important physicochemical process in biological system.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesized Gold Nanoclusters and Iron Complexes as Scaffolds for Multimodal Cancer Bioimaging

Zhao

Rehman

et al. 2016

Small

Self Cite

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Cancer treatment has a far greater chance of success if the neoplasm is diagnosed before the onset of metastasis to vital organs. Hence, cancer early diagnosis is extremely important and remains a major challenge in modern therapeutics. In this contribution, facile and new method for rapid multimodal tumor bioimaging is reported by using biosynthesized iron complexes and gold nanoclusters via simple introduction of AuCl and Fe ions. The observations demonstrate that the biosynthesized Au nanoclusters may act as fluorescent and computed tomography probes for cancer bioimaging while the iron complexes behave as effective contrast agent for magnetic resonance imaging. The biosynthesized iron complexes and gold nanoclusters are found biocompatible in vitro (MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay) and in vivo for all the vital organs of circulatory and excretory system. These observations raise the possibility that the biosynthesized probes may find applications in future clinical diagnosis for deep seated early neoplasms by multimodal imaging.

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A lysosomal targeting fluorescent probe and its zinc imaging in SH-SY5Y human neuroblastoma cells

Duan

Ding

Huang

et al. 2019

Chinese Chemical Letters

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Novel in situ biosynthesized fluorescent zinc nanoclusters for specific cellular bio-imaging

Cited by 11 publications

References 15 publications

In situSynthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

In situSynthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

Biosynthesized Gold Nanoclusters and Iron Complexes as Scaffolds for Multimodal Cancer Bioimaging

A lysosomal targeting fluorescent probe and its zinc imaging in SH-SY5Y human neuroblastoma cells

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