Novel indirect enzyme-linked immunosorbent assay (ELISA) method to detect Total E. coli in water environment

Wang, Na; He, Miao; Shi, Hanchang

doi:10.1016/j.aca.2007.03.041

Cited by 38 publications

(39 citation statements)

References 14 publications

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“…Antibodies consist of two regions: the variable region and the constant region (stem of the Y). It is believed that the variable region of the antibody is defined for antigen binding, and the constant region reacts with effective cells or molecules [5]. The antibody immobilization on the biosensor was achieved by reacting amino groups of the constant regions of the antibody with the succinimidyl ester of the linker to form covalent amide bonds.…”

Section: Immobilization Of Antibody On Swcnt-based Biosensormentioning

confidence: 99%

“…Polymerase chain reaction (PCR), quantitative real-time PCR (qPCR), and enzyme-linked immunosorbent assay (ELISA) methods have improved both the speed and sensitivity of pathogen detection, compared with detection by the traditional culturing methods [5,6]. However, PCR techniques often have high risk of false results owing to inhibition by components of the sample matrix and complicated pretreatment process such as extraction of the whole or a part of the pathogen DNA [5]. ELISA technique also requires extra-labeled antibodies that are costly and time-consuming steps [7].…”

Section: Introductionmentioning

confidence: 99%

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Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Choi

Lee

Park

et al. 2017

Journal of Food Quality

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The goal of this research is to develop a single-walled carbon nanotube-(SWCNT-) based biosensor to detect Staphylococcus aureus. The specificity of 11 bacteria and polyclonal anti-Staphylococcus aureus antibodies (pAbs) was determined using an indirect ELISA. The pAbs were immobilized onto sensor platform after the hybridization of 1-pyrenebutanoic acid succinimidyl ester (PBASE). The resistance difference (Δ ) was calculated using a potentiostat. The bacteria detected by the biosensor were observed using a scanning electron microscope (SEM). The optimum concentration of SWCNTs on the platform was determined to be 0.1 mg/mL. The binding of pAbs with S. aureus resulted in a significant increase in resistance value of the biosensor ( < 0.05). The SEM images confirmed the specific binding of S. aureus on the biosensor. The SWCNT-based biosensor was able to detect S. aureus with a limit of detection (LOD) of 4 log CFU/mL.

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Section: Immobilization Of Antibody On Swcnt-based Biosensormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Choi

Lee

Park

et al. 2017

Journal of Food Quality

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“…Therefore, several methods have emerged to realize the rapid detection and enumeration of E. coli bacteria in drinking water such as Immunological [15][16][17][18] and Polymerase Chain Reaction [19][20][21].…”

Section: Emerging Methodsmentioning

confidence: 99%

The Detection Method ofEscherichia coliin Water Resources: A Review

Nurliyana

Sahdan

Wibowo

et al. 2018

J. Phys.: Conf. Ser.

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“…The lower layer is a sterile chromatography paper, on which a certain amount of lactose, indicators (bromcresol purple and TTC), peptone, and fungistat can be absorbed. [5].…”

Section: Detection Methodsmentioning

confidence: 99%

Design of a Constant-Temperature Incubator for Paper Strip Contamination Detection

Lv¹,

Yu²,

Liu³

2015

Proceedings of the 2015 International Conference on Mechatronics, Electronic, Industrial and Control Engineering

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Abstract-Currently, hygienic conditions of public tableware and kitchenware have become a hot social concern. This work aimed at solving existing problems in detecting the presence of bacteria, a constant-temperature incubator for the paper strip method was designed and a related temperature-control system was developed. Firstly, the detection method and working principle of the incubator were elaborated upon. Using a paper strip, Escherichia coli (E. coli) was collected from the tableware to be examined. The paper strip was then placed in the incubator for a constant-temperature culture. Through chromogenic reaction, the hygienic condition of the tableware could be qualitatively and quantitatively assessed by analyzing the image information on the paper strip. Subsequently, with regard to the heater band, a wrapping pattern was designed and a suitable material was chosen. Three types of temperature sensors were compared, and a platinum resistor was finally selected and installed at the proper position. Additionally, an electronic thermostat was chosen. Conclusively, the designed incubator, which integrated the constant-temperature culture and image acquisition system, could detect the temperature accurately, reliably, and conveniently in real time, and could also detect the hygienic condition of public tableware rapidly and effectively.

show abstract

Novel indirect enzyme-linked immunosorbent assay (ELISA) method to detect Total E. coli in water environment

Cited by 38 publications

References 14 publications

Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

The Detection Method ofEscherichia coliin Water Resources: A Review

Design of a Constant-Temperature Incubator for Paper Strip Contamination Detection

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