Novel indole C-glycosides from Isatis indigotica and their potential cytotoxic activity

Wu, Yixuan; Zhenxue, Zhang; Hu, Hui; Li, Dongmei; Qiu, Guofu; Hu, Xianming; He, Xiangjiu

doi:10.1016/j.fitote.2010.10.016

Cited by 76 publications

(45 citation statements)

References 20 publications

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“…(Cruciferae), is an important traditional Chinese medicine mainly used for the treatment of influenza and infection diseases 1 . Previous studies showed that extracts of “ban lan gen” had diverse biological activities and contained various types of active constituents2, 3, 4, 5, 6, 7, 8, 9. However, the previous studies were mainly focused on ethanol or methanol extracts, which is not in agreement with practical application of the herbal medicine by decocting with water.…”

Section: Introductionmentioning

confidence: 98%

Aromatic compounds from an aqueous extract of “ban lan gen” and their antiviral activities

Liu

Chen

Guo

et al. 2017

Acta Pharmaceutica Sinica B

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A pair of new diphenyl glycerol ether enantiomers (−)-1 and (+)-1 and two new methyl benzamidobenzoates 2 and 3, named (−)-(R)- and (+)-(S)-isatindigotrioic acid [(−)-1 and (+)-1] and isatindigoticamides A (2) and B (3), respectively, were isolated from an aqueous decoction of the roots of Isatis indigotica (ban lan gen). Their structures were elucidated by spectroscopic data analysis including 2D NMR experiments. The absolute configurations of (−)-1 and (+)-1 were assigned based on the CD exciton chirality method. Compounds 2 and 3 exhibited antiviral activities against HSV-1 with IC50 values of 4.87 and 25.87 μmol/L, respectively. Compound 2 was also found active against Coxsackie virus B3 and LPS-induced NO production.

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Section: Introductionmentioning

confidence: 98%

Aromatic compounds from an aqueous extract of “ban lan gen” and their antiviral activities

Liu

Chen

Guo

et al. 2017

Acta Pharmaceutica Sinica B

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“…Around 100 chemical constituents with different structural types (sulfur-containing alkaloids, lignans, and flavonoids, etc.) and various biological activities were isolated from the extracts7, 8, 9, 10, 11, 12, 13, 14, 15. However, chemical studies were dominated by extracting “ban lan gen” with ethanol or methanol, differing from practically decocting with water.…”

Section: Introductionmentioning

confidence: 99%

Indole alkaloid sulfonic acids from an aqueous extract of Isatis indigotica roots and their antiviral activity

Meng

Guo

Liu

et al. 2017

Acta Pharmaceutica Sinica B

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Six new indole alkaloid sulfonic acids (1–6), together with two analogues (7 and 8) that were previously reported as synthetic products, were isolated from an aqueous extract of the Isatis indigotica root. Their structures including the absolute configurations were determined by spectroscopic data analysis, combined with enzyme hydrolysis and comparison of experimental circular dichroism and calculated electronic circular dichroism spectra. In the preliminary assay, compounds 2 and 4 showed antiviral activity against Coxsackie virus B3 and influenza virus A/Hanfang/359/95 (H3N2), respectively.

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“…(Cruciferae) is a traditional medical plant widely cultivated in China (Angelini et al, 2007) and is popular in pharmaceuticals, food additives, flavors, and other industrial materials due to abundant bio-active components, including alkaloids (Wu et al, 1997, 2011), flavonoids (Deng et al, 2008), organic acids, and glycosides (Liau et al, 2007). Until now, only a few references mentioned gene expression and functions in the developmental process and accumulation of metabolites responding to abiotic or biotic stress treatments in which the reference genes were nothing more than UBQ , ACT and 18s r RNA (Han et al, 2013; Li Q. et al, 2014; Chen et al, 2015; Dong et al, 2015; Han, 2015; Hu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Li¹,

Jing²,

Lu³

et al. 2017

Front. Plant Sci.

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Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants. Based on the Minimum Information for Publication of Real-Time Quantitative PCR Experiments (MIQE) guidelines, it is necessary to select and validate putative appropriate reference genes for qRT-PCR normalization. In the current study, three algorithms, geNorm, NormFinder, and BestKeeper, were applied to assess the expression stability of 10 candidate reference genes across five different tissues and three different abiotic stresses in Isatis indigotica Fort. Additionally, the IiYUC6 gene associated with IAA biosynthesis was applied to validate the candidate reference genes. The analysis results of the geNorm, NormFinder, and BestKeeper algorithms indicated certain differences for the different sample sets and different experiment conditions. Considering all of the algorithms, PP2A-4 and TUB4 were recommended as the most stable reference genes for total and different tissue samples, respectively. Moreover, RPL15 and PP2A-4 were considered to be the most suitable reference genes for abiotic stress treatments. The obtained experimental results might contribute to improved accuracy and credibility for the expression levels of target genes by qRT-PCR normalization in I. indigotica.

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Novel indole C-glycosides from Isatis indigotica and their potential cytotoxic activity

Cited by 76 publications

References 20 publications

Aromatic compounds from an aqueous extract of “ban lan gen” and their antiviral activities

Aromatic compounds from an aqueous extract of “ban lan gen” and their antiviral activities

Indole alkaloid sulfonic acids from an aqueous extract of Isatis indigotica roots and their antiviral activity

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

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