Novel Insights into the Effect of Pythium Strains on Rapeseed Metabolism

Bělonožníková, Kateřina; Vaverová, Kateřina; Vaněk, Tomáš; Kolařík, Miroslav; Hýsková, Veronika; Vaňková, Radomı́ra; Dobrev, Petre I.; Křížek, Tomáš; Hodek, Ondřej; Čokrtová, Kateřina; Štípek, Adam; Ryšlavá, Helena

doi:10.3390/microorganisms8101472

Cited by 14 publications

(12 citation statements)

References 61 publications

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“…Phytohormones in the tobacco leaves were determined as described previously (Belonoznikova et al, 2020; Prerostova et al, 2020). They were extracted and separated on a reverse‐phase cation exchange SPE column (Oasis‐MCX, Waters) into the acid fraction (auxins, abscisic acid [ABA], salicylic acid [SA], jasmonic acid [JA]) by elution with methanol and into the basic fraction (cytokinins [CKs] and 1‐aminocyclopropane‐1‐carboxylic acid [ACC]) by elution with 0.35 M NH 4 OH in 60% methanol (Belonoznikova et al, 2020, Prerostova et al, 2020). The fractions were analysed on a C18 high performance liquid chromatography (HPLC) column (Ultimate 3000, Dionex) coupled to a 3200 Q TRAP hybrid triple quadrupole/linear ion trap mass spectrometer (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

“…Phytohormones in the tobacco leaves were determined as described previously (Belonoznikova et al, 2020;Prerostova et al, 2020). They were extracted and separated on a reverse- (Belonoznikova et al, 2020, Prerostova et al, 2020. The fractions were analysed on a C18 high performance liquid chromatography (HPLC) column (Ultimate 3000, Dionex) coupled to a 3200 Q TRAP hybrid triple quadrupole/linear ion trap mass spectrometer (Applied Biosystems).…”

Section: Phytohormone Analysismentioning

confidence: 99%

Section: Phytohormone Analysismentioning

confidence: 99%

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Casein as protein and hydrolysate: Biostimulant or nitrogen source for Nicotiana tabacum plants grown in vitro?

Bělonožníková

Černý

Hýsková

et al. 2023

Physiologia Plantarum

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In contrast to inorganic nitrogen (N) assimilation, the role of organic N forms, such as proteins and peptides, as sources of N and their impact on plant metabolism remains unclear. Simultaneously, organic biostimulants are used as priming agents to improve plant defense response. Here, we analysed the metabolic response of tobacco plants grown in vitro with casein hydrolysate or protein. As the sole source of N, casein hydrolysate enabled tobacco growth, while protein casein was used only to a limited extent. Free amino acids were detected in the roots of tobacco plants grown with protein casein but not in the plants grown with no source of N. Combining hydrolysate with inorganic N had beneficial effects on growth, root N uptake and protein content. The metabolism of casein‐supplemented plants shifted to aromatic (Trp), branched‐chain (Ile, Leu, Val) and basic (Arg, His, Lys) amino acids, suggesting their preferential uptake and/or alterations in their metabolic pathways. Complementarily, proteomic analysis of tobacco roots identified peptidase C1A and peptidase S10 families as potential key players in casein degradation and response to N starvation. Moreover, amidases were significantly upregulated, most likely for their role in ammonia release and impact on auxin synthesis. In phytohormonal analysis, both forms of casein influenced phenylacetic acid and cytokinin contents, suggesting a root system response to scarce N availability. In turn, metabolomics highlighted the stimulation of some plant defense mechanisms under such growth conditions, that is, the high concentrations of secondary metabolites (e.g., ferulic acid) and heat shock proteins.

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Section: Methodsmentioning

confidence: 99%

“…Phytohormones in the tobacco leaves were determined as described previously (Belonoznikova et al, 2020;Prerostova et al, 2020). They were extracted and separated on a reverse- (Belonoznikova et al, 2020, Prerostova et al, 2020. The fractions were analysed on a C18 high performance liquid chromatography (HPLC) column (Ultimate 3000, Dionex) coupled to a 3200 Q TRAP hybrid triple quadrupole/linear ion trap mass spectrometer (Applied Biosystems).…”

Section: Phytohormone Analysismentioning

confidence: 99%

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Casein as protein and hydrolysate: Biostimulant or nitrogen source for Nicotiana tabacum plants grown in vitro?

Bělonožníková

Černý

Hýsková

et al. 2023

Physiologia Plantarum

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“…Animal feed can be processed from suitable raw materials using cellulases for increased nutritive value and digestibility of cereals (Sathya & Khan, 2014). Fungal cellulases can be used in the agricultural sector explicitly as regulators of seed metabolism (Bělonožníková et al, 2020) in addition to improving the plant performance against pathogens (Kuhad et al, 2011).…”

Section: Cellulasesmentioning

confidence: 99%

Plant-associated endophytic fungi as potential bio-factories for extracellular enzymes: Progress, Challenges and Strain improvement with precision approaches

Raghav

Jyoti

Siddiqui

et al. 2022

Journal of Applied Microbiology

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There is an intricate network of relations between endophytic fungi and their hosts that affects the production of various bioactive compounds. Plant-associated endophytic fungi contain industrially important enzymes and have the potential to fulfil their rapid demand in the international market to boost business in technology.Being safe and metabolically active, they have replaced the usage of toxic and harmful chemicals and hold a credible application in biotransformation, bioremediation and industrial processes. Despite these, there are limited reports on fungal endophytes that can directly cater to the demand and supply of industrially stable enzymes. The underlying reasons include low endogenous production and secretion of enzymes from fungal endophytes which have raised concern for widely accepted applications. Hence, it is imperative to augment the biosynthetic and secretory potential of fungal endophytes. Modern state-of-the-art biotechnological technologies aiming at strain improvement using cell factory engineering as well as precise gene editing like Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and its Associated proteins (Cas) systems which can provide a boost in fungal endophyte enzyme production. Additionally, it is vital to characterize optimum conditions to grow one strain with multiple enzymes (OSME). The present review encompasses various plants-derived endophytic fungal enzymes and their applications in various sectors. Furthermore, we postulate the feasibility of new precision approaches with an aim for strain improvement and enhanced enzyme production.

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“…Examples of the efficacy of P. oligandrum against the plant pathogens have been reported for P. ultimum, P. aphanidermatum, Fusarium oxysporum , Verticillium albo-atrum, Rhizoctonia solani and Physperma solani ( Benhamou et al., 1999 , 2012 ). Bělonožníková et al. (2020) described differences among 12 diverse strains of P. oligandrum with regard to their properties and effect on plants, and identified strain M1 with positive influence on plant fitness.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Pythium oligandrum on improvement of lucerne yield, root development and disease score under field conditions

et al. 2022

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IntroductionBiological control of root diseases of lucerne (Medicago sativa L.) has potential benefits for stand performance but this remains unsupported by evidence from practical field studies.MethodsIn field experiments at three sites our objectives were to determine the effect of Pythium oligandrum, as spring, autumn and intensive regime treatments on (i) lucerne plant density and root traits development, and (ii) forage yield and forage traits. Lucerne stands were managed under two or three treatments: non-treated control and P. oligandrum applied at two intensities of application under four-cut utilization.Results and discussionUnder relatively dry conditions (annual mean 10°C and <500 mm precipitation) lucerne dry matter yield was significantly reduced by 6%, which could be related to mechanisms of inappropriate stimulation and disturbance of the balance between auxins and ethylene. Under annual precipitation of >500 mm, positive impacts on stand height or fine root mass were observed for the autumn and intensive treatments where positive root response was visible only in alluvial soil. However, these changes did not result in higher yield and probably more applications per year will be needed for significant forage yield improvement. This study highlights the limits of field-scale biological control in which the potential of P. oligandrum for lucerne productivity improvement was realised only under a humid environment or deep alluvial soils, where higher root disease infestation may also be expected.

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Novel Insights into the Effect of Pythium Strains on Rapeseed Metabolism

Cited by 14 publications

References 61 publications

Casein as protein and hydrolysate: Biostimulant or nitrogen source for Nicotiana tabacum plants grown in vitro?

Casein as protein and hydrolysate: Biostimulant or nitrogen source for Nicotiana tabacum plants grown in vitro?

Plant-associated endophytic fungi as potential bio-factories for extracellular enzymes: Progress, Challenges and Strain improvement with precision approaches

Efficacy of Pythium oligandrum on improvement of lucerne yield, root development and disease score under field conditions

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