Novel insights into the inhibitory mechanism of (+)-catechin against trimethylamine-N-oxide demethylase

Li, Yingchang; Song, Suzhen; Li, Yuanyuan; Du, Fengxia; Li, Shuangyan; Lǐ, Jiànróng

doi:10.1016/j.foodchem.2021.131559

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…The apparent kinetic parameters of CIFE on COX-2 and HAse were determined, including Michaelis constant (Km) and maximum reaction velocity (Vm), according to the methods of the COX-2 and HAase inhibition assays above. Then, based on the Michaelis Menten equation, the Lineweaver Burk double reciprocal equation was used to plot and analyze the effects of different concentrations of CIFE and substrates (hematin, sodium hyaluronate) on the reaction rate ( Li et al, 2022 ). The reversibility of CIFE on COX-2 and HAase, as well as the types of reversible inhibition reactions, were determined.…”

Section: Methodsmentioning

confidence: 99%

Targeted screening of multiple anti-inflammatory components from Chrysanthemi indici Flos by ligand fishing with affinity UF-LC/MS

Huang,

Xiong,

Zhang

et al. 2024

Front. Pharmacol.

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Chrysanthemi indic Flos (CIF) has been commonly consumed for the treatment of inflammation and related skin diseases. However, the potential bioactive components responsible for its anti-inflammatory and sensitive skin (SS) improvement activities, and the correlated mechanisms of action still remain unknown. In this work, it was firstly found that the CIF extract (CIFE) displayed arrestive free radical scavenging activity on DPPH and ABTS radicals, with no significant difference with positive control Trolox (p > 0.05). Then, compared to the negative group, CIFE markedly decreased the productions of the pro-inflammatory cytokines (IL-1β, IL-6, PEG2, TNF-α, IFN-γ, NO) in LPS induced RAW264.7 cells in a dose-dependent manner (p < 0.01). Besides, CIFE strongly inhibited the COX-2 and hyaluronidase (HAase) with the IC50 values of 1.06 ± 0.01 μg/mL and 12.22 ± 0.39 μg/mL, indicating higher inhibitory effect than positive control of aspirin of 6.33 ± 0.05 μg/mL (p < 0.01), and comparable inhibitory effect with indometacin of 0.60 ± 0.03 μg/mL, and ascorbic acid of 11.03 ± 0.41 μg/mL (p > 0.05), respectively. Furthermore, kinetic assays with Lineweaver-Burk plot (Michaelis Menten equation) suggested that CIFE reversibly inhibited the COX-2 and HAase, with a mixed characteristics of competitive and non-competitive inhibition. Thereafter, multi-target affinity ultrafiltration liquid chromatography-mass spectrometry (UF-LC/MS) method was employed to fast fish out the potential COX-2 and HAase in CIFE. Herein, 13 components showed various affinity binding degrees to the COX-2 and HAase, while those components with relative binding affinity (RBA) value higher than 3.0, such as linarin and chlorogenic acid isomers, were deemed to be the most bioactive components for the anti-inflammatory and SS improvement activities of CIFE. Finally, the interaction mechanism, including binding energy, inhibition constant, docking sites, and the key amino acids involved in hydrogen bonds between the potential ligands and COX-2/HAase were simulated and confirmed with the molecule docking analysis. In summary, this study showcased the prominent anti-inflammatory and SS improvement activities of CIF, which would provide further insights on this functional medicinal plant to be a natural anti-SS remedy.

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Section: Methodsmentioning

confidence: 99%

Targeted screening of multiple anti-inflammatory components from Chrysanthemi indici Flos by ligand fishing with affinity UF-LC/MS

Huang,

Xiong,

Zhang

et al. 2024

Front. Pharmacol.

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Reaction‐Based Fluorescence Assays for the Determination of Food Trimethylamine Oxide

Peng,

Huang,

Wang

et al. 2024

Luminescence

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Trimethylamine oxide (TMAO), a microbial metabolite commonly found in foods, has been attracting increasing attention as it is associated with the risk of several diseases. Simple and accurate analytical methods are crucial for TMAO study. In the present study, we proposed a chemical reaction‐based fluorescence assay for TMAO detection using synthetic small molecular probes. After systematic screening and optimization, the sensitive and selective quantification of TMAO has been achieved based on a fluorescence probe P6 (3‐iodopropanyl group modified resorufin). Excellent linearity (R2 = 0.997) was found between 6.25 and 50 μM, and the limit of detection (LOD) was 0.20 μM. Using this method, TMAO levels in several marine fishes and shellfishes have been successfully analyzed. The probe‐based assay offers a simple and useful way for TMAO determination. The design is inspired by the unique oxidation reaction between TMAO and halogen, which opens a new perspective in the development of more advanced analytical assays for TMAO in the future.

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In vivo mechanism of the interaction between trimethylamine lyase expression and glycolytic pathways

Li,

Wu,

Song

et al. 2025

Food Funct.

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Novel insights into the inhibitory mechanism of (+)-catechin against trimethylamine-N-oxide demethylase

Cited by 3 publications

References 53 publications

Targeted screening of multiple anti-inflammatory components from Chrysanthemi indici Flos by ligand fishing with affinity UF-LC/MS

Targeted screening of multiple anti-inflammatory components from Chrysanthemi indici Flos by ligand fishing with affinity UF-LC/MS

Reaction‐Based Fluorescence Assays for the Determination of Food Trimethylamine Oxide

In vivo mechanism of the interaction between trimethylamine lyase expression and glycolytic pathways

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