Novel insights on the role of VCAM-1 and ICAM-1: Potential biomarkers for cardiovascular diseases

Kaur, Rupinder; Singh, Varinder; Kumari, Pratima; Singh, Ravinder J.; Chopra, Hitesh; Emran, Talha Bin

doi:10.1016/j.amsu.2022.104802

Cited by 19 publications

(7 citation statements)

References 19 publications

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“…Elevated expression levels of cytokines and chemokines involved in leukocyte recruitment and activation, as well as elevated expression levels of adhesion molecules that promote extravasation of mediators into the tissue, influence transplantation outcomes by modulating donor-specific immune responses ( 21 ). In comparison to non-perfused heart tissue, transcript levels of IL-6, IL-8, and TNF-α were strongly upregulated in non-TD, shNS-transduced, and shβ2m+CIITA-transduced hearts and showing higher gene expression levels in the atrium than in the ventricle.…”

Section: Resultsmentioning

confidence: 99%

“…TNF-α production is further stimulated by high levels of IL-6 ( 54 ). Simultaneous upregulation of adhesion molecules (ICAM-1 and V-CAM-1) during EVMP promotes leukocyte-endothelial interactions and subsequent immune cell infiltration into cardiac tissue ( 21 ). The increase in IL-6, IL-8, and TNF-α levels has already been described in several studies and showed a similar course to cardiopulmonary bypass or extracorporeal membrane oxygenation ( 54 , 55 ).…”

Section: Discussionmentioning

confidence: 99%

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Heart immunoengineering by lentiviral vector-mediated genetic modification during normothermic ex vivo perfusion

Schmalkuche,

Rother,

Burgmann

et al. 2024

Front. Immunol.

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Heart transplantation is associated with major hurdles, including the limited number of available organs for transplantation, the risk of rejection due to genetic discrepancies, and the burden of immunosuppression. In this study, we demonstrated the feasibility of permanent genetic engineering of the heart during ex vivo perfusion. Lentiviral vectors encoding for short hairpin RNAs targeting beta2-microglobulin (shβ2m) and class II transactivator (shCIITA) were delivered to the graft during two hours of normothermic EVHP. Highly efficient genetic engineering was indicated by stable reporter gene expression in endothelial cells and cardiomyocytes. Remarkably, swine leucocyte antigen (SLA) class I and SLA class II expression levels were decreased by 66% and 76%, respectively, in the vascular endothelium. Evaluation of lactate, troponin T, and LDH levels in the perfusate and histological analysis showed no additional cell injury or tissue damage caused by lentiviral vectors. Moreover, cytokine secretion profiles (IL-6, IL-8, and TNF-α) of non-transduced and lentiviral vector-transduced hearts were comparable. This study demonstrated the ex vivo generation of genetically engineered hearts without compromising tissue integrity. Downregulation of SLA expression may contribute to reduce the immunogenicity of the heart and support graft survival after allogeneic or xenogeneic transplantation.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Heart immunoengineering by lentiviral vector-mediated genetic modification during normothermic ex vivo perfusion

Schmalkuche,

Rother,

Burgmann

et al. 2024

Front. Immunol.

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“…From the above, only VCAM-1 plays a major part in both the early and the later stages of atherosclerosis. As a consequence, it provides a suitable and easy target for diagnosing pathological situations [ 161 , 162 , 163 ]. Another modulator associated with cardiovascular disease is PTX3.…”

Section: The Role Of Inflammatory Mediators In Cardiovascular Diseasementioning

confidence: 99%

Inflammatory Mediators of Endothelial Dysfunction

Dri

Lampas

Lazaros

et al. 2023

Life

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Endothelial dysfunction (ED) is characterized by imbalanced vasodilation and vasoconstriction, elevated reactive oxygen species (ROS), and inflammatory factors, as well as deficiency of nitric oxide (NO) bioavailability. It has been reported that the maintenance of endothelial cell integrity serves a significant role in human health and disease due to the involvement of the endothelium in several processes, such as regulation of vascular tone, regulation of hemostasis and thrombosis, cell adhesion, smooth muscle cell proliferation, and vascular inflammation. Inflammatory modulators/biomarkers, such as IL-1α, IL-1β, IL-6, IL-12, IL-15, IL-18, and tumor necrosis factor α, or alternative anti-inflammatory cytokine IL-10, and adhesion molecules (ICAM-1, VCAM-1), involved in atherosclerosis progression have been shown to predict cardiovascular diseases. Furthermore, several signaling pathways, such as NLRP3 inflammasome, that are associated with the inflammatory response and the disrupted H2S bioavailability are postulated to be new indicators for endothelial cell inflammation and its associated endothelial dysfunction. In this review, we summarize the knowledge of a plethora of reviews, research articles, and clinical trials concerning the key inflammatory modulators and signaling pathways in atherosclerosis due to endothelial dysfunction.

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“…This triggers the activation of nuclear transcription factor-kappa beta (NF-κβ) which in-turn up-regulates the transcription of adhesion molecules; selectin-E, intracellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). The association between ICAM-1and incidences of cardiovascular diseases has been previously established 10 . In detailed, ICAM-1 is expressed on the surface of endothelial cells in response to tissue damage and inflammation 11 .…”

Section: Introductionmentioning

confidence: 99%

Ubiquinol attenuates γ-radiation induced coronary and aortic changes via PDGF/p38 MAPK/ICAM-1 related pathway

El-Sabbagh,

Fadel,

El-Hazek

et al. 2023

Sci Rep

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Endothelial vascular injury is one of the most pivotal disorders emerging during radiotherapy. It is crucial to rely on strong antioxidants to defend against vascular damage. The current study was carried out to investigate the ameliorative effect of ubiquinol (Ubq) against gamma (γ)-radiation induced aortic and coronary changes, with highlighting its role in suppression of p38 mitogen activated protein kinase (MAPK). Exposure to γ-radiation was adopted as a potent detrimental model that induces vascular tissue damage. Concisely, male albino rats were irradiated at a dose level of 7 Gy and treated daily with Ubq (10 mg/kg/day, p.o.) for 7 days pre-and post-irradiation. At the end of the experiment, lipid profile, 8-hydroxydeoxyguanosine (8-OHdG), gene expression of intercellular adhesion molecule (ICAM-1), platelet derived growth factor (PDGF), p38 MAPK and matrix metalloproteinase-9 (MMP-9) were estimated. Exposure to radiation significantly deteriorates aortic and coronary tissues. Conversely, administration of Ubq significantly reduced serum t-cholesterol, LDL and triglycerides (p = 0.001). In addition, Ubq prevented oxidative DNA damage (8-OHdG) (p = 0.1) and reduced serum MMP-9 (p = 0.001) which contributed to the endothelial cells damage. The positive impact of Ubq was more apparent in suppression of both PDGF (p = 0.001) and p38 MAPK (p = 0.1) protein concentrations, leading subsequently in reduction of ICAM-1 (p = 0.001) gene expression. As a conclusion, vascular endothelial damage brought on by γ-radiation is one of the leading causes of coronary and aortic deteriorations which could be successfully mitigated by Ubq.

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Novel insights on the role of VCAM-1 and ICAM-1: Potential biomarkers for cardiovascular diseases

Cited by 19 publications

References 19 publications

Heart immunoengineering by lentiviral vector-mediated genetic modification during normothermic ex vivo perfusion

Heart immunoengineering by lentiviral vector-mediated genetic modification during normothermic ex vivo perfusion

Inflammatory Mediators of Endothelial Dysfunction

Ubiquinol attenuates γ-radiation induced coronary and aortic changes via PDGF/p38 MAPK/ICAM-1 related pathway

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