Novel Intranasal Vaccine Delivery System by Chitosan Nanofibrous Membrane Containing N-Terminal Region of Ipad Antigen as a Nasal Shigellosis Vaccine, Studies in Guinea Pigs

Jahantigh, Danial; Saadati, Mojtaba; Fasihi-Ramandi, Mahdi; Mousavi, Mahdieh; Zand, A.M.

doi:10.1016/s1773-2247(14)50005-6

Cited by 24 publications

(13 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It can interact with mucus and epithelial cells to induce a redistribution of cytoskeletal F-actin, increasing the permeability of the epithelium. Thus, both chemical and physical methods have been used to prepare chitosan-based particles loaded with proteins; however, major drawbacks are associated with the use of chemical cross-linking methods (14).…”

Section: Introductionmentioning

confidence: 99%

Serum Anti-Vibrio cholerae Immunoglobulin Isotype in BALB/c Mice Immunized With ompW-Loaded Chitosan

Fasihi-Ramandi¹,

Ghobadi-Ghadikolaee²,

Ahmadi-Renani³

et al. 2016

Int J Enteric Pathog

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Serum Anti-Vibrio cholerae Immunoglobulin Isotype in BALB/c Mice Immunized With ompW-Loaded Chitosan

Fasihi-Ramandi¹,

Ghobadi-Ghadikolaee²,

Ahmadi-Renani³

et al. 2016

Int J Enteric Pathog

View full text Add to dashboard Cite

show abstract

“…The high surface area/volume ratio of these fibers, combined with their potential biocompatibility and biodegradable nature offers tremendous promise for biomedical applications such as targeted vaccine delivery [23]. These membranes which were produced through the process of electrospinning contained the N-terminal region of the IpaD Shigella subunit antigen [24]. These membranes which were produced through the process of electrospinning contained the N-terminal region of the IpaD Shigella subunit antigen [24].…”

Section: Nanofibersmentioning

confidence: 99%

“…In one study, the vaccine involved the delivery of Shigella antigens through a non-woven chitosan nanofibrous membrane [24]. This solution was used for electrospinning [24]. An aqueous of chitosan (untreated and hydrolyzed)/ AcOH solution and the diluted recombinant antigen were used in all encapsulation processes, in a 1:1 volume ratio, to create a final recombinant antigen concentration of 0.5 mg/mL.…”

Section: Nanofibersmentioning

confidence: 99%

Biodegradable polymers for modern vaccine development

Bose

Kim

Chang

et al. 2019

Journal of Industrial and Engineering Chemistry

View full text Add to dashboard Cite

A B S T R A C TMost traditional vaccines are composed either of a whole pathogen or its parts; these vaccines, however, are not always effective and can even be harmful. As such, additional agents known as adjuvants are necessary to increase vaccine safety and efficacy. This review summarizes the potential of biodegradable materials, including synthetic and natural polymers, for vaccine delivery. These materials are highly biocompatible and have minimal toxicity, and most biomaterial-based vaccines delivering antigens or adjuvants have been shown to improve immune response, compared to formulations consisting of the antigen alone. Therefore, these materials can be applied in modern vaccine development.

show abstract

“…In this experimental study, different strains of used bacteria were prepared in Tehran University and confirmed by biochemical and serological methods (15)(16)(17). In order to produce lipopolysaccharide, each strain of Shigella bacterium was linearly cultured in tertiary agar culture medium (40 g/L) and placed in a temperature of 37°C for one day.…”

Section: Preparation Culturing and Bacterial Lysismentioning

confidence: 99%

Investigation of Intestinal Enzyme Activity and Effects of Non-starch Polysaccharide on it

Piryaei¹,

Motamedi²,

Far³

2018

IJET

View full text Add to dashboard Cite

Background and Purpose: Shigella is a human shigellosis and its lipopolysaccharide is identified by 4TLR. The 4TLR is a family of pseudo-TOLL receptors and many immune routes are triggered by stimulating these receptors. Many studies show increasing of 4TLR expression in Mesenchyme stem cells under the influence of lipopolysaccharide. The main objective of this study was to identify the appropriate lipopolysaccharide of Shigella strains by stimulating the immune system for vaccine studies. Materials and Methods: In this experimental study, the stem cell of human Mesenchymal derived from bone marrow was treated by three dilution of 0.1, 0.01, and 0.001 extract of Shigella strains (Flexneri, Dysentery and Sonnei) containing lipopolysaccharide. Then, the expression of 4TLR at RNA level was evaluated by RT-PCR and Q-PCR techniques. Cells treated with phosphate buffer saline were considered as control group. Findings: The expression of 4TLR was observed in all treatments groups except for treatment groups with relative concentration of 0.001 sonnei and dysentery as well as control group. Changes in 4TLR expression were dose-dependent on all treatment groups. The highest expression was related to the treatment with Shigella Flexneri extract and the smallest was related to Shigella sonnei. The use of pure lipopolysaccharide of Escherichia coli as a positive control showed that the lipopolysaccharide in Shigella extract is responsible for increasing the expression of 4TLR. Conclusion: given the increased expression of 4TLR by Shigella extract, this extract is recommended to increase the efficacy of the vaccine.

show abstract

Novel Intranasal Vaccine Delivery System by Chitosan Nanofibrous Membrane Containing N-Terminal Region of Ipad Antigen as a Nasal Shigellosis Vaccine, Studies in Guinea Pigs

Cited by 24 publications

References 31 publications

Serum Anti-Vibrio cholerae Immunoglobulin Isotype in BALB/c Mice Immunized With ompW-Loaded Chitosan

Serum Anti-Vibrio cholerae Immunoglobulin Isotype in BALB/c Mice Immunized With ompW-Loaded Chitosan

Biodegradable polymers for modern vaccine development

Investigation of Intestinal Enzyme Activity and Effects of Non-starch Polysaccharide on it

Contact Info

Product

Resources

About