Novel layer-by-layer self-assembled peptide nanocarriers for siRNA delivery

Abstract

Novel stable diphenylalaninamide peptide based nanocarriers were designed by layer-by-layer polyelectrolyte deposition to load siRNA for gene silencing.

Targeted delivery of microRNA‐199a‐3p using self‐assembled dipeptide nanoparticles efficiently reduces hepatocellular carcinoma in mice

“…Next, we evaluated the miRNA loading capacity of RΔF‐LA NPs and its release from the NPs under in vitro conditions. The loading capacity of NPs (2.9%) was reasonably high and compared well with earlier reported delivery systems . An enhanced release of miRNA at pH 5 as compared to pH 7.4 or in serum was observed.…”

“…The loading capacity of NPs (2.9%) was reasonably high and compared well with earlier reported delivery systems. (36,37) An enhanced release of miRNA at pH 5 as compared to pH 7.4 or in serum was observed. This could be due to reduced charge -charge interactions between RDF-LA NPs and miRNA at pH 5; at this pH the negative charge of miRNA molecules is likely to be significantly reduced.…”

Layer-by-layer siRNA/poly(L-lysine) Multilayers on Polydopamine-coated Surface for Efficient Cell Adhesion and Gene Silencing

“…Layer-by-layer (LBL) self-assembly has been recognized as a simple and versatile method to fabricate thin polyelectrolyte multilayers via the sequential deposition of oppositely charged polyelectrolytes 22 – 25 . The LBL technique can efficiently embed various electrolytic biomolecules, including genes, proteins and inorganic particles, in a multilayer structure 26 – 28 . Also, the loaded molecules can be released in a sustained manner during the hydration and dissolution of the multilayer in an aqueous solution.…”

Primary biocompatibility tests of poly(lactide-co-glycolide)-(poly-L-orithine/fucoidan) core–shell nanocarriers

“…The LbL self-assembly technique can combine different polyelectrolyte materials and nanoparticles (NPs) to construct a carrier with an ultra-thin film multilayer structure. Through the precise control of various parameters, such as membrane structure, film thickness, and chemical and mechanical properties, it can deliver and release the drug into tumour cells [1][2][3]. The controlled release effect of LbL self-assembled drug carriers is primarily manifested through the following: (1) the environmentdependent multilayer assembly characteristics determine the controlled release of the drug by regulating the lamellar membrane structure; (2) the assembly characteristics of a variety of polymers include the inclusion of hydrolysis, enzymolysis, stimulus response, etc.…”