Novel liquid chromatographic–tandem mass spectrometric methods using silica columns and aqueous–organic mobile phases for quantitative analysis of polar ionic analytes in biological fluids

Weng, Naidong; Shou, Wilson Z.; Chen, Yu-Luan; Jiang, Xuxian

doi:10.1016/s0378-4347(01)00021-4

Cited by 116 publications

(63 citation statements)

References 35 publications

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“…Nicotine/cotinine samples were analyzed using procedures adapted from Naidong et al (2001). Briefly, a liquid chromatography (LC)/tandem mass spectrometry method validated for selectivity, calibration model fit, sensitivity, accuracy, and precision using a Micromass Quatro II LC mass spectrometer was used.…”

Section: Methodsmentioning

confidence: 99%

Withdrawal from Chronic Nicotine Administration Impairs Contextual Fear Conditioning in C57BL/6 Mice

Davis¹,

James²,

Siegel³

et al. 2005

J. Neurosci.

142

256

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The effects of acute nicotine administration (0.09 mg/kg nicotine), chronic nicotine administration (6.3 mg/kg/d nicotine for 14 d), and withdrawal from chronic nicotine administration on fear conditioning in C57BL/6 mice were examined. Mice were trained using two coterminating conditioned stimulus (30 s; 85 dB white noise)-unconditioned stimulus (2 s; 0.57 mA foot shock) pairings and tested 24 h later for contextual and cued fear conditioning. Acute nicotine administration enhanced contextual fear conditioning, chronic nicotine administration had no effect on contextual fear conditioning, and withdrawal from chronic nicotine administration impaired contextual fear conditioning. Plasma nicotine concentrations were similar after acute and chronic treatment and were within the range reported for smokers. During withdrawal, concentrations of nicotine were undetectable. An acute dose of nicotine (0.09 mg/kg) during withdrawal from chronic nicotine treatment reversed withdrawal-associated deficits in contextual fear conditioning. The results suggest that tolerance to the effects of nicotine on contextual fear conditioning develops with chronic nicotine treatment at a physiologically relevant dose, and withdrawal from this chronic nicotine treatment is associated with impairments in contextual fear conditioning. These findings provide a model of how the effects of nicotine on learning may contribute to the development and maintenance of nicotine addiction.

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Section: Methodsmentioning

confidence: 99%

Withdrawal from Chronic Nicotine Administration Impairs Contextual Fear Conditioning in C57BL/6 Mice

Davis¹,

James²,

Siegel³

et al. 2005

J. Neurosci.

142

256

View full text Add to dashboard Cite

show abstract

“…Urine samples from Days 1 through 5 were stored at −70 °C and analyzed for the nicotine metabolite cotinine using an adaptation of a method reported elsewhere (Naidong, Shou, Chen, & Jiang, 2001; limit of quantitation = 1.0 ng/ml). Samples from Days 1, 3, and 5 only were analyzed for metabolites of the TSNAs NNK (NNAL and NNAL-glucuronide reported here as total NNAL or NNAL-T) using liquid chromatography-mass spectrometry/mass spectrometry (MDS Pharma Services, Lincoln, NE; method described in Roethig et al, 2007; limit of quantitation = 20 pg/ml).…”

Section: Urine Cotinine and Nnalmentioning

confidence: 99%

Evaluating oral noncombustible potential-reduced exposure products for smokers

Blank

Eissenberg

2010

Nicotine & Tobacco Research

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“…In addition, APCI is much less sensitive than is ESI to interference from matrix on ionization, specifically salts and ion pairing agents present in the mobile phase used for reverse phase HPLC analyses. However, the use of silica based columns operated under hydrophilic interaction chromatography mode (HILIC) has resulted in an increase in the sensitivity of LC-ESI/MS/MS detection of basic compounds [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Analysis of [3′,3′-d2]-nicotine and [3′,3′-d2]-cotinine by capillary liquid chromatography–electrospray tandem mass spectrometry

Murphy

Villalta

et al. 2007

Journal of Chromatography B

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A selective and sensitive LC/MS/MS assay was developed for the quantification of d 2 -nicotine and d 2 -cotinine in plasma of current and past smokers administered d 2 -nicotine. After solid phase extraction and liquid liquid extraction, HPLC separation was achieved on a capillary hydrophilic interaction chromatography phase column. The analytes were monitored by tandem mass spectrometry with electrospray positive ionization. Linear calibration curves were generated for d 2 -nicotine (0.03 to 6.0 ng/ml plasma) and d 2 -cotinine (0.15 to 25 ng/ml plasma). The lower limits of quantitation were 0.15 ng/ml and 0.25 ng/ml for d 2 -nicotine and d 2 -cotinine, respectively. The coefficient of variation was 3.7% for d 2 -nicotine and 2.5% for d 2 -cotinine. The method was applied to two ongoing studies of d 2 -nicotine metabolism in prior and current smokers. Preliminary analysis of a subset of subjects from these studies detected a significantly lower rate of nicotine conversion to cotinine by past smokers compared to current smokers.

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Novel liquid chromatographic–tandem mass spectrometric methods using silica columns and aqueous–organic mobile phases for quantitative analysis of polar ionic analytes in biological fluids

Cited by 116 publications

References 35 publications

Withdrawal from Chronic Nicotine Administration Impairs Contextual Fear Conditioning in C57BL/6 Mice

Withdrawal from Chronic Nicotine Administration Impairs Contextual Fear Conditioning in C57BL/6 Mice

Evaluating oral noncombustible potential-reduced exposure products for smokers

Analysis of [3′,3′-d2]-nicotine and [3′,3′-d2]-cotinine by capillary liquid chromatography–electrospray tandem mass spectrometry

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