Novel localization of orexin A in the tubular cytotypes of the rat testis

Tafuri, Simona; Muto, R.; Pavone, Luigi Michele; Valiante, Salvatore; Costagliola, Anna; Staiano, Norma; Vittoria, Alfredo

doi:10.1016/j.regpep.2010.06.011

Cited by 21 publications

(32 citation statements)

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“…In particular, we demonstrated that the neuroendocrine cells of cattle urethra, the principal cells of rat epididymis, and the Sertoli cells of rat testis provide a local source of OxA in this tract (Russo et al 2008;Pavone et al 2009;Tafuri et al 2009Tafuri et al , 2010. Moreover, the expression of Ox1R mRNAs has been shown in the epididymis, seminal vesicles, and penis of humans (Karteris et al 2004), in the testis of sheep (Zhang et al 2005), chicken (Okubo et al 2003), and rat (Jören et al 2001;Barreiro et al 2004Barreiro et al , 2005, in the cattle prostate (Russo et al 2008), and in the rat epididymis ).…”

Section: Introductionmentioning

confidence: 80%

“…A distribution similar to that of Ox1R-expressing cells has been previously reported for OxA in the tubular compartment of rat testis. The peptide OxA was detected in resting, leptotene, zygotene, and pachytene spermatocytes (Barreiro et al 2005), as well as in Sertoli cells and spermatids at stages of the germinal cycle ranging from the VIIth to the XIVth (Tafuri et al 2010).…”

Section: Resultsmentioning

confidence: 99%

“…The peptide produced by the Sertoli cells, which probably represent the principal source of OxA in the whole gonad (Barreiro et al 2005;Tafuri et al 2010), may either act on its same source by an autocrine modality of action, or may be spread out towards the neighbouring germ cells through a paracrine diffusion. In rat testis tissue, OxA prevents the secretion of the stem cell factor (SCF) by Sertoli cells (Barreiro et al 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Orexins and their receptors have also been found in brain regions other than hypothalamus and in several peripheral organs, thus suggesting multiple functions for such peptides (Kirchgessner and Liu 1999;Jören et al 2001;Voisin et al 2003;Ehrström et al 2005;Russo et al 2008;Pavone et al 2009;Tafuri et al 2009Tafuri et al , 2010. However, a major involvement of OxA and its receptor Ox1R versus OxB and Ox2R has been demonstrated in a number of physiological and pathological conditions of both the central nervous system and peripheral organs.…”

Section: Introductionmentioning

confidence: 99%

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Expression and role of receptor 1 for orexins in seminiferous tubules of rat testis

et al. 2012

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Orexins (OxA and OxB) and their receptors (Ox1R and Ox2R), originally detected in the hypothalamus, have also been localized in multiple cerebral areas and peripheral organs. Thus, in addition to their central function in the regulation of food intake, arterial blood pressure, heart rate, sleep/wake cycle, sexual behaviour, arousal, and hypothalamic/hypophyseal axis, these neuropeptides may exert a local action in various peripheral organs and tissues. Emerging evidence suggests a main role of OxA and its highly specific receptor Ox1R in the male genital tract of mammals. We previously demonstrated OxA localization in Sertoli cells and spermatids of rat testis. Here, we show positive stainings of Ox1R in developing spermatocytes, and spermatids of rat testis by immunohistochemistry. The expression of Ox1R mRNA and the protein in the tissue was also established by reverse-transcription polymerase chain reaction and Western blotting respectively. The addition of OxA to fresh testis slices significantly increased testosterone (T) secretion which, conversely, was inhibited by Mullerian inhibiting substance (MIS). The sequential treatment of testis samples with the two substances highlighted an antagonizing activity of OxA versus MIS in regulating T levels. Furthermore, the stimulating effect on T production by OxA was prevented by the addition of the selective Ox1R inhibitor SB-408124. Overall, our findings suggest that locally secreted OxA interacting with Ox1R activates signals which antagonize MIS action in the control of T levels in mammalian testis.

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Section: Introductionmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression and role of receptor 1 for orexins in seminiferous tubules of rat testis

et al. 2012

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“…Total RNA extraction from testis samples, the synthesis of cDNA, and RT-PCR for the detection of CD68 transcripts were performed according to the procedure previously described [17,18]. The following specific primers were used: forward 5 0 -CATCCTTCACG-GAGACACCT-3 0 and reverse 5 0 -GGGAATGAGAGAGCCAAGTG-3 0 for CD68; forward 5 0 -GAGGCTCAGAGCAAGAGAGG-3 0 and reverse 5 0 -TGACATCTCGCACAATCTCC-3 0 for b-actin.…”

Section: Rt-pcr and Immunoblotting Analysesmentioning

confidence: 99%

Expression of the CD68 glycoprotein in the rat epididymis

et al. 2015

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Orexin/Hypocretin Signaling

Kukkonen

2016

Current Topics in Behavioral Neurosciences

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Orexin/hypocretin peptide (orexin-A and orexin-B) signaling is believed to take place via the two G-protein-coupled receptors (GPCRs), named OX and OX orexin receptors, as described in the previous chapters. Signaling of orexin peptides has been investigated in diverse endogenously orexin receptor-expressing cells - mainly neurons but also other types of cells - and in recombinant cells expressing the receptors in a heterologous manner. Findings in the different systems are partially convergent but also indicate cellular background-specific signaling. The general picture suggests an inherently high degree of diversity in orexin receptor signaling.In the current chapter, I present orexin signaling on the cellular and molecular levels. Discussion of the connection to (potential) physiological orexin responses is only brief since these are in focus of other chapters in this book. The same goes for the post-synaptic signaling mechanisms, which are dealt with in Burdakov: Postsynaptic actions of orexin. The current chapter is organized according to the tissue type, starting from the central nervous system. Finally, receptor signaling pathways are discussed across tissues, cell types, and even species.

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Novel localization of orexin A in the tubular cytotypes of the rat testis

Cited by 21 publications

References 32 publications

Expression and role of receptor 1 for orexins in seminiferous tubules of rat testis

Expression and role of receptor 1 for orexins in seminiferous tubules of rat testis

Expression of the CD68 glycoprotein in the rat epididymis

Orexin/Hypocretin Signaling

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