Novel method for cell penetration based on MEMS technology with failure and impacting characterization of mouse ooctye membrane

Gilani, A. Ghanadzadeh; Koohsorkhi, Javad; Shamloo, Amir

doi:10.1016/j.sna.2017.06.008

Cited by 3 publications

(1 citation statement)

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“…The hyper-elastic model utilized in the current study was also used to characterize the mechanical response of the mouse ZP. The study utilized Micro Electro Mechanical Mechanism System (MEMS) technology to directly measure forces acting on the ZP and identify, by FE analysis, the material model parameters 33 . The study reports values of 0.67, which are of the same order obtained in the current study for the human ZP.…”

Section: Discussionmentioning

confidence: 99%

Zona pellucida shear modulus, a possible novel non-invasive method to assist in embryo selection during in-vitro fertilization treatment

Priel

Szaingurten-Solodkin

et al. 2020

Sci Rep

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eliahu Levitas 3 & iris Har-Vardi 3* the present study investigated the association between oocyte zona pellucida shear modulus (ZpSM) and implantation rate (IR). Ninety-three oocytes collected from 38 in-vitro fertilization patients who underwent intracytoplasmic sperm injection were included in this case-control study. the Zp was modeled as an isotropic compressible hyperelastic material with parameter C 10 , which represents the ZpSM. computational methodology was used to calculate the mechanical parameters that govern Zp deformation. fifty-one developed embryos were transferred and divided into two groups-implanted and not implanted. Multivariate logistic regression analysis was performed to identify the association between ZpSM and iR while controlling for confounders. Maternal age and number of embryos per transfer were significantly associated with implantation. The IR of embryos characterized by C 10 values in the range of 0.20-0.40 kPa was 66.75%, while outside this range it was 6.70%. This range was significantly associated with implantation (p < 0.001). Geometric properties were not associated with implantation. Multivariate logistic regression analysis that controlled for relevant confounders indicated that this range was independently associated with implantation (adjusted OR 38.03, 95% confidence interval 4.67-309.36, p = 0.001). The present study suggests that ZPSM may improve the classic embryo selection process with the aim of increasing iR. The zona pellucida (ZP) is a non-cellular layer of glycoproteins surrounding the oocyte 1. Over the years, numerous studies have described an association between the physical state of a cell and its function or fate 2-5. One of the most studied physical properties of oocytes is zona hardening, resulting from cortical granule release during fertilization 6. This physical property, aimed to prevent polyspermy 6,7 , also occurs spontaneously in response to post-ovulatory aging, and results in poor fertilization rates 8-10. Furthermore, spontaneous zona hardening is associated with exposure to in-vitro culture conditions 11,12 that may consequently hinder the blastocyst's potential to hatch the ZP. Although many studies have investigated animal oocytes or embryos, their observations link physical parameters to fertilization and developmental potential and not to implantation potential. Several approaches have been described in the literature to measure the mechanic properties of the ZP 13 by oocyte indentation 14,15 , compression 16 , or aspiration 17,18. Based on these capabilities, two studies have used the physical inputs as selection criteria for embryos. Murayama et al. measured mouse ZP elasticity using a micro tactile sensor (MTS) system. They demonstrated specific changes in this elasticity during oocyte maturation, fertilization, and embryo development, and concluded that the MTS system can be applied to assisted reproductive technology (ART) to evaluate embryo quality 19. Yanez et al. studied the mechanistic properties of human and

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Section: Discussionmentioning

confidence: 99%