Novel methylotrophic isolates from lake sediment, description of Methylotenera versatilis sp. nov. and emended description of the genus Methylotenera

Abstract

Phylogenetic positions, and genotypic and phenotypic characteristics of three novel methylotrophic isolates, strains 301 T , 30S and SIP3-4, from sediment of Lake Washington, Seattle, USA, are described. The strains were restricted facultative methylotrophs capable of growth on single carbon compounds (methylamine and methanol) in addition to a limited range of multicarbon compounds. All strains used the N-methylglutamate pathway for methylamine oxidation. Strain SIP3-4 possessed the canonical (MxaFI) methanol dehydrogenase, but strains 301 T and 30S did not. All three strains used the ribulose monophosphate pathway for C1 assimilation. The major fatty acids in the three strains were C 16 : 0 and C 16 : 1 v7c. The DNA G+C contents of strains 301 T and SIP3-4 were 42.6 and 54.6 mol%, respectively. Based on 16S rRNA gene sequence phylogeny and the relevant phenotypic characteristics, strain SIP3-4 was assigned to the previously defined species Methylovorus glucosotrophus. Strains 301 T and 30S were closely related to each other (100 % 16S rRNA gene sequence similarity) and shared 96.6 % 16S rRNA gene sequence similarity with a previously described isolate, Methylotenera mobilis JLW8 T . Based on significant genomic and phenotypic divergence with the latter, strains 301 T and 30S represent a novel species within the genus Methylotenera, for which the name Methylotenera versatilis sp. nov. is proposed; the type strain is 301 T

Core-satellite populations and seasonality of water meter biofilms in a metropolitan drinking water distribution system

“…Drinking water distribution system microbiota F Ling et al Among these taxa, Methylomicrobium, Methylobacter and Methylocystis are methanotrophs, and isolated strains have been reported of using methane as the sole or preferred carbon source (Kalyuzhnaya et al, 2008;Bowman et al, 1993;Belova et al, 2013). For methanol utilization, Methylotenera are capable of using methanol as the sole carbon source (Kalyuzhnaya et al, 2012), and Hyphomicrobium and Methylobacterium are known as facultative methylotrophs (Chistoserdova et al, 2009). Other facultative methylotrophy-related OTUs are also present in the core communities, such as Mycobacterium and Pseudomonas.…”

An Integrated Proteomics/Transcriptomics Approach Points to Oxygen as the Main Electron Sink for Methanol Metabolism in Methylotenera mobilis

“…This genus represents methylotrophs that are not only sufficiently divergent in genomics terms from the formerly described Methylophilaceae but also differ from them by not possessing one of the hallmark enzymes of methylotrophy, methanol dehydrogenase (16,19). However, Methylotenera species are still able, albeit weakly, to grow on methanol (16,17).…”

“…However, none of these genes appeared to respond to methanol, which suggests that oxygen may be the main electron sink during growth on methanol. This study identifies initial targets for future focused physiological studies, including mutant analysis, which will provide further details into this novel process.The Methylotenera genus is a recent addition to the family Methylophilaceae (16,17). This genus represents methylotrophs that are not only sufficiently divergent in genomics terms from the formerly described Methylophilaceae but also differ from them by not possessing one of the hallmark enzymes of methylotrophy, methanol dehydrogenase (16,19).…”

“…The Methylotenera genus is a recent addition to the family Methylophilaceae (16,17). This genus represents methylotrophs that are not only sufficiently divergent in genomics terms from the formerly described Methylophilaceae but also differ from them by not possessing one of the hallmark enzymes of methylotrophy, methanol dehydrogenase (16,19).…”

Microbial Diversity of the Trichloroethylene Contaminated Groundwater Treatment System and Characterization of Pseudomonas sp. DHC8

“…세균군집 분석결과 11.5%를 차지한 Methylotenera 속과 각각 3.8%를 차지한 Methylobacillus, Methylophilus 속은 β -proteobacteria의 methylotroph 그룹에 속하는 미생물로 methanol 과 methylamine을 탄소원으로 이용한다고 알려져 있다 (Kalyuzhnaya et al, 2006(Kalyuzhnaya et al, , 2012. 미량의 methanol 유입은 외부 탄소원으로서 의 이용가능성을 배제할 수 없으며, 세균군집구조에 대한 영향 이 있을 수 있다.…”