Novel micro-straw for freezing small quantities of human spermatozoa

Huang, Chuan; Gan, Run-Xin; Zhang, Huan; Zhou, Wen-Jun; Huang, Zeng-Hui; Jiang, Shaosong; Ji, Xi-Ren; Gong, Fei; Fan, Liqing; Zhu, Wenbing

doi:10.1016/j.fertnstert.2020.03.032

Cited by 14 publications

(12 citation statements)

References 23 publications

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“…Actually, many carriers including Cryoloop, Cryotop, and Cell sleeper, can be used in clinical cases or severe oligoasthenospermia. In this study, we choose Micro‐straw, Cryoplus, and LSL carriers for two reasons: first, those carriers are reported in recent years and they are manufactured in China, which are widely used in reproductive centers; in addition, those carriers have been used for clinical treatment in China and achieved successful pregnancies through ICSI, which makes the results of this study more convinced, their clinical outcomes were shown in Table S1 13,14,18 . The Micro‐straw carrier was introduced to our center in 2015 and cutted by 0.25 ml straw.…”

Section: Discussionmentioning

confidence: 99%

“…The freezing method of Micro‐straw carrier (Figure 1A) was similar to that used in our previous study, 13 but we optimized the freezeing–thawing method by using different cooling and thawing rates. In brief, 10 μl sperm‐cryoprotectant mixture was loaded into the Micro‐straw, and it was placed 1 cm above LN 2 for 70 s before immersion.…”

Section: Methodsmentioning

confidence: 99%

“…For instance, in our recent study, we developed the so‐called “Micro‐straw” to cryopreserve rare sperm from severe oligospermia sufferers or epididymal and testicular sperm from epididymal sperm aspiration (PESA) and testicular sperm extraction (TESE). As a result, 52 deliveries were achieved after ICSI using the frozen–thawed spermatozoa 13 …”

Section: Introductionmentioning

confidence: 99%

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Micro‐straw: An efficient cryopreservation carrier for rare human spermatozoa

Luo

Huang

et al. 2022

Andrology

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Background: Many cryopreservation carriers have been introduced to freeze rare human spermatozoa, however, these carriers relative attributes and comparative effectivenesses have not yet been systematically studied.Objectives: Is the micro-straw cryopreservation carrier more effective for cryopreserving rare human spermatozoa compared with the Cryoplus and a new micro-straw (LSL straw) carriers?Materials and methods: This study involves 93 samples from healthy sperm donors and 40 samples from patients diagnosed with oligospermia, asthenospermia, oligoasthenospermia, or obstructive azoospermia. We determined the optimal freeze-thaw protocol for the Micro-straw carrier. The post-thaw survival rate, normal sperm morphology, acrosome integrity, and DNA fragmentation for Micro-straw, Cryoplus, and LSL carriers were then determined. Finally, we verified the effects of freezing using these carriers by comparing the qualities of post-thaw spermatozoa from patients. Results:The highest total motility (TM) and progressive motility (PR) survival rates were obtained by placing the Micro-straw at 1 cm above the LN 2 surface for 70 s during freezing and in a 42 • C water bath for 40 s during thawing. No differences were observed in the PR survival rate, acrosome integrity, and DNA fragmentation of the post-thaw spermatozoa from the three carriers. However, the normal morphology rate of spermatozoa frozen using the Micro-straw carrier was higher than for the Cryoplus carrier (p < 0.05), and the TM survival rate of spermatozoa frozen with the Micro-straw was higher than that for the LSL carrier (p < 0.01). In verification tests, there were no significant differences in the quality of post-thaw spermatozoa cryopreserved using these carriers for both rare spermatozoa or epididymal sperm.Discussion and conclusion: Micro-straw, Cryoplus, and LSL carriers are all efficient means of freezing rare human spermatozoa. However, the Micro-straw carrier is more economical, safe, and user-friendly.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Micro‐straw: An efficient cryopreservation carrier for rare human spermatozoa

Luo

Huang

et al. 2022

Andrology

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“…Several other studies reported achieving live births following ICSI with spermatozoa cryopreserved with various other carriers. 6 8 …”

Section: Introductionmentioning

confidence: 99%

Clinical benefits of a modified Cryopiece system for cryopreservation of rare ejaculated and testicular spermatozoa for ICSI

Xu¹,

Li²,

Chen³

et al. 2022

Asian Journal of Andrology

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Cryopreservation of rare testicular-retrieved spermatozoa for intracytoplasmic sperm injection (ICSI) in patients with severe oligozoospermia and azoospermia remains a major challenge in clinical practice. This study evaluated the Cryopiece system as a potential technique to cryopreserve rare human spermatozoa for ICSI. Small numbers of ejaculated (24 patients) and testicular (13 patients) spermatozoa were cryopreserved using the Cryopiece system. The total number of recovered spermatozoa and motility were assessed after thawing. Thirty-seven couples underwent ICSI using spermatozoa cryopreserved by the Cryopiece system, and ICSI outcomes (rates of fertilization, embryo cleavage, and clinical pregnancy) were evaluated. The average sperm post-thaw retrieval rate was 79.1%, and motility was 29.7%. Ejaculated spermatozoa had a higher post-thaw motility (32.5%) than testicular spermatozoa (21.8%; P = 0.005). ICSI achieved a fertilization rate of 61.9%, embryo cleavage rate of 84.6%, and clinical pregnancy rate of 43.3%. The ICSI outcomes in the ejaculated and testicular frozen-thawed spermatozoa were similar. Assisted oocyte activation (AOA) after ICSI with motile (72.1%) or immotile (71.9%) spermatozoa resulted in a significantly higher fertilization rate than that when using motile spermatozoa without AOA (52.0%; P = 0.005). However, AOA did not enhance the clinical pregnancy rate (55.6% or 40.0% vs 35.3%; P = 0.703). The Cryopiece system is simple and useful for the cryopreservation of small numbers of ejaculated or testicular spermatozoa for ICSI in patients with severe oligozoospermia or nonobstructive azoospermia.

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“…To date, several sperm cryocarriers have been developed, including human or hamster empty zonae (Desai et al, 2004;Walmsley et al, 1998), micro-straw (Huang et al, 2020;Isachenko et al, 2005), ICSI needle (Sohn et al, 2003), Cryotop (Chen et al, 2015), Cellsleeper (Endo et al, 2012) and Cryopiece (Sun et al, 2017).…”

mentioning

confidence: 99%

Cryopreservation of human spermatozoa by freezing testicular seminiferous tubule with novel cryopiece

Feng

Zheng

2021

Andrologia

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The amount of spermatozoa recovered by techniques such as testicular sperm extraction (TESE) (Erdem et al., 2017) in patients with obstructive azoospermia (OA) is very limited. The testicular spermatozoa injected into oocytes by the intracytoplasmic sperm injection (ICSI) technique form embryos that are transferred into the uterus to obtain a clinical pregnancy. The success of TESE and ICSI in obtaining fertility in patients has been known for a long time (An et al., 2018).Although the amount of spermatozoa obtained by testicular biopsy is small, the retrieved spermatozoon is a valuable resource for patients with azoospermia (Desai et al., 2004). Indeed, the success of assisted reproductive therapy is often unpredictable, failure implies the need for uncomfortable and painful repeated testicular biopsies, and this can also increase the risk of orchitis, disruption of the bloodtestis barrier, and production of antisperm antibodies (Schlegel & Su, 1997). Cryopreservation of testicular spermatozoa allows patients to perform multiple treatments of assisted reproduction with a small amount of testicular spermatozoa (Liu & Li, 2020). Therefore, in order to avoid damage to the testis caused by repeated surgery, there is the need to freeze testicular spermatozoa found in the first testicular biopsy.

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Novel micro-straw for freezing small quantities of human spermatozoa

Cited by 14 publications

References 23 publications

Micro‐straw: An efficient cryopreservation carrier for rare human spermatozoa

Micro‐straw: An efficient cryopreservation carrier for rare human spermatozoa

Clinical benefits of a modified Cryopiece system for cryopreservation of rare ejaculated and testicular spermatozoa for ICSI

Cryopreservation of human spermatozoa by freezing testicular seminiferous tubule with novel cryopiece

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