Novel MicroRNA Regulators of Atrial Natriuretic Peptide Production

Wu, Connie; Arora, Pankaj; Agha, Obiajulu; Hurst, L; Allen, Kaitlin; Nathan, Daniel I.; Hu, Dongjian; Jiramongkolchai, Pawina; Smith, J. G.; Melander, Olle; Trenson, Sander; Janssens, Stefan; Domian, Ibrahim J.; Wang, Thomas J.; Bloch, Kenneth D.; Buys, Emmanuel; Bloch, Donald B.; Newton‐Cheh, Christopher

doi:10.1128/mcb.01114-15

Cited by 22 publications

(19 citation statements)

References 37 publications

(52 reference statements)

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“…Although it was not detected in the present study, several researches in vivo and in vitro have demonstrated that excessive fluid volume would cause cardiomyocyte injury and apoptosis, which could induce a series of cardiovascular dysfunctions by impairing biochemical signal transduction, vascular tone adaptation, and baroreflex control of circulation . When HD patients are fluid overloaded, the sustained preload‐induced cardiomyocyte injury might lead to abnormal expression and production of bioactive substances that could disrupt BP regulation . However, the exact mechanism involved was not yet adequately established.…”

Section: Discussionmentioning

confidence: 66%

Effect of Improving Volume Overload on Home Blood Pressure Variability in Hemodialysis Patients

et al. 2019

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Blood pressure variability is an independent predictor of adverse clinical events in hemodialysis patients. Volume overload is one of the most important factors affecting blood pressure homeostasis. In the present study, we explored the effects of dry weight reduction on home blood pressure variability in volume overload hemodialysis patients. Hemodialysis patients with volume overload had their dry weight gradually decreased under the guidance of bioimpedance methods, which was represented by calf‐bioimpedance ratio (Calf‐BR). Home blood pressure was measured on waking up and at bedtime for 1 week at baseline and at the end of the two‐month study. Coefficient of variation was used to define home blood pressure variability. Thirty‐eight hemodialysis patients had their dry weight significantly decreased from 60.7 ± 11.3 to 59.6 ± 10.7 kg (P = 0.003) accompanied with a significant reduction in calf‐BR (0.828 ± 0.023 vs. 0.786 ± 0.020, P<0.001). The systolic and diastolic blood pressure decreased significantly. Moreover, the whole‐day, morning, and evening systolic blood pressure variability gradually and significantly decreased by the end of the study (5.6 ± 2.1% versus 4.0 ± 1.7%, P<0.001; 7.7 ± 3.5% vs. 6.3 ± 2.7%, P = 0.005; 7.5 ± 2.8% vs. 5.9 ± 2.3%, P = 0.002, respectively). Diastolic blood pressure variability parameters were unchanged. The interdialytic weight gain and the incidence of adverse events were similar throughout the study period. Gradual dry weight reduction by bioimpedance methods improved home blood pressure variability in hemodialysis patients with chronic fluid overload.

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Section: Discussionmentioning

confidence: 66%

Effect of Improving Volume Overload on Home Blood Pressure Variability in Hemodialysis Patients

et al. 2019

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“…COS7 cells (obtained directly from American Type Culture Collection, CRL-1651) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 200 U/ml penicillin, and 200 μg/ml streptomycin. Cardiomyocytes (CM) were differentiated from human embryonic stem cells (hESC, WA07, NIH Human Embryonic Stem Cell Registry Number 0067, generated by WiCell Research Institute) as previously described [ 10 ]. Human ESC-CMs have been well characterized and display functional and structural properties of primary human cardiomyocytes [ 11 , 12 ], including specifically their expression of NPPA , a hallmark of cardiomyocyte-specific expression.…”

Section: Methodsmentioning

confidence: 99%

“…Deep sequencing analysis identified rs61764044, a second variant located 123 nucleotides downstream of, and perfectly correlated with, rs5068 (r 2 = 1); the minor alleles of both variants are always co-inherited. Because of this perfect linkage disequilibrium, rs61764044 is also associated with increased plasma ANP levels, lower blood pressure, and reduced risk of hypertension [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…We have previously shown that rs5068 decreases NPPA transcriptional repression by disrupting the binding site of miR-425 in the NPPA 3’UTR [ 9 ]. Similarly, rs61764044 introduced a G-U “wobble” base pairing within the binding region of miR-155 to the NPPA 3’UTR, thereby conferring resistance to miR-155-mediated repression of NPPA expression [ 10 ]. In human cardiomyocytes, overexpression of either miR-425 or miR-155 induced a significant decrease in NPPA expression [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-425 and microRNA-155 cooperatively regulate atrial natriuretic peptide expression and cGMP production

et al. 2018

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AimsAtrial natriuretic peptide (ANP), secreted primarily by atrial cardiomyocytes, decreases blood pressure by raising cyclic 3’,5’-guanosine monophosphate (cGMP) levels and inducing vasorelaxation, natriuresis, and diuresis. Raising the level of ANP has been shown to be an effective treatment for hypertension. To advance the future development of an anti-microRNA (miR) approach to increasing expression of ANP, we investigated the regulation of NPPA expression by two miRs: miR-425 and miR-155. We examined whether miR-425 and miR-155 have an additive effect on the expression and function of ANP.Methods and resultsHuman embryonic stem cell-derived cardiomyocytes (hESC-CMs) were transfected with miR-425, miR-155, or a combination of the two miRs. Two days later, NPPA expression was measured using real time qPCR. Each of the miRs decreased NPPA expression over a wide range of concentrations, with a significant reduction at concentrations as low as 1 nM. The combination of miR-425 and miR-155 reduced NPPA expression to a greater extent than either miR-425 or miR-155 alone. An in vitro assay was developed to study the potential biological significance of the miR-induced decrease in NPPA expression. The cooperative effect of miR-425 and miR-155 on NPPA expression was associated with a significant decrease in cGMP levels.ConclusionsThese data demonstrate that miR-425 and miR-155 regulate NPPA expression in a cooperative manner. Targeting both miRNAs with anti-miRs (possibly at submaximal concentrations) might prove to be a more effective strategy to modulate ANP levels, and thus blood pressure, than targeting either miRNA alone.

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“…Currently, the ideas concerning local humoral mechanisms of BP regulation are being successfully developed, and the progress in this direction is quite significant (Burnier and Forni 2012, Mangiafico et al 2013, Markov 2005, Ruilope et al 2010. The role of kidneys and heart in BP maintenance is known and well-researched (Carlström et al 2015, Esler 2015, Qu et al 2016, Roman et al 2016, Wu et al 2016. The significance of the tonus of sections of autonomic nervous system in BP formation is discussed in (Bakris and Nathan 2014, Bhatt et al 2014, Mu et al 2011.…”

Section: Introductionmentioning

confidence: 99%

Complex assessment of blood pressure regulation system in hypertension patients

Samorodskaya¹,

Polischuk²,

Елисеева³

2019

RRP

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Introduction. There are almost no studies characterizing the integrative level of blood pressure (BP) regulation. Materials and methods. 277 people of both genders aged 58.6±6.4 with stage II hypertension disease were randomized into six groups. The monotherapy of hypertension disease was conducted in five groups, using nebivolol, lisinopril, indapamide, amlodipine, and losartan. The sixth group had a combined therapy (lisinopril/indapamide). The therapy effectiveness was assessed at four levels of blood pressure regulation, using the following methods: 1) laser Doppler flowmetry, determination of the level of tumor necrosis factor-α and interleukin-10; 2) echocardiography and Doppler sonography, ultrasound examination of the renal blood flow, ECG, Holter monitoring of ECG; 3) an examination of the heart rate variability level and a quantitative assessment of beta-adrenoreception of erythrocyte cell membranes; 4) the regulatory and adaptive status was assessed, using the method of cardio-respiratory synchronism. Results and discussion. A more significant BP decrease was revealed during a combination therapy (by 20.4% of the baseline daily value). At the integrative level, an index of the regulatory and adaptive status (iRAS) increased in the treatment with lisinopril/indapamide combination (by 40.5%), amlodipine (by 40.5%), losartan (by 35.3%), and lisinopril (by 30.2%). Nebivolol administration resulted in a 13.5% decrease in iRAS. Indapamide therapy had no significant effect on iRAS. Conclusion. A comprehensive assessment of the blood pressure regulation system makes it possible to control the effectiveness of the therapy not only on a target organ or function, but also on the condition of the organism as an integral system.

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Novel MicroRNA Regulators of Atrial Natriuretic Peptide Production

Cited by 22 publications

References 37 publications

Effect of Improving Volume Overload on Home Blood Pressure Variability in Hemodialysis Patients

Effect of Improving Volume Overload on Home Blood Pressure Variability in Hemodialysis Patients

MicroRNA-425 and microRNA-155 cooperatively regulate atrial natriuretic peptide expression and cGMP production

Complex assessment of blood pressure regulation system in hypertension patients

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