Novel microsatellite loci in the grass snake (<i>Natrix natrix</i>) and cross‐amplification in the dice snake (<i>Natrix tessellata</i>)

Meister, Barbara; Armbruster, Georg F. J.; Frauenfelder, Nathalie; Baur, Bruno

doi:10.1111/j.1755-0998.2008.02460.x

Cited by 8 publications

(5 citation statements)

References 11 publications

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“…Sixty‐eight samples were genotyped using 13 polymorphic microsatellite loci in a novel combination (Supporting Information, Table S4). Nine of these loci were either developed for Natrix natrix or had already been tested for their applicability for this species (Hille et al ., ; Meister et al ., ). Yet, only two loci characterized by Hille et al .…”

Section: Methodsmentioning

confidence: 97%

Integrative taxonomy provides evidence for the species status of the Ibero-Maghrebian grass snakeNatrix astreptophora

Felix

Kindler

Ivanov

et al. 2016

Biol. J. Linn. Soc.

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The grass snake (Natrix natrix) is Europe's most widely distributed and, in many regions, most common snake species, with many morphologically defined subspecies. Yet, the taxonomy of grass snakes is relatively little studied and recent work has shown major conflicts between morphologically defined subspecies and phylogeographical differentiation. Using external morphology, osteological characters, and information from 13 microsatellite loci and two mitochondrial markers, we examine differentiation of the subspecies N. n. astreptophora from the North African Maghreb region, the Iberian Peninsula and neighbouring France. According to previous studies, N. n. astreptophora corresponds to a deeply divergent mitochondrial clade and constitutes the sister taxon of all remaining grass snakes. In the French Pyrenees region, there is a contact zone of N. n. astreptophora with another subspecies, N. n. helvetica. Our analyses of microsatellites and mitochondrial DNA reveal that the distribution ranges of the two taxa abut there, but both hybridize only exceptionally. Even though many morphological characters are highly variable and homoplastic in grass snakes, N. n. astreptophora differs consistently from all other grass snakes by its reddish iris coloration and in having significantly fewer ventral scales and another skull morphology. Considering further the virtual absence of gene flow between N. n. astreptophora and N. n. helvetica, and acknowledging the morphological distinctiveness of N. n. astreptophora and its sister group relationship to all remaining subspecies of grass snakes, we conclude that Natrix astreptophora (Seoane, 1884) should be recognized as a distinct species. Further research is needed to explore whether N. astreptophora is polytypic because a single sample of N. astreptophora from Tunisia turned out to be genetically highly distinct from its European conspecifics.

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Section: Methodsmentioning

confidence: 97%

Integrative taxonomy provides evidence for the species status of the Ibero-Maghrebian grass snakeNatrix astreptophora

Felix

Kindler

Ivanov

et al. 2016

Biol. J. Linn. Soc.

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“…Genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide‐based extraction protocol, and genotyped using a set of nine microsatellites (Natnat01, Natnat05, Natnat06, Natnat08, Natnat09, Natnat11, µNt3, µNt7, and µNt8new) developed for the grass snake or the dice snake ( Natrix tessellata ), respectively (Gautschi, Widmer & Koella, 2000; Meister et al ., 2009). Polymerase chain reaction amplification and electrophoresis of amplified products were conducted as described by Meister et al . (2009).…”

Section: Methodsmentioning

confidence: 99%

Spatial genetic analysis of the grass snake, Natrix natrix (Squamata: Colubridae), in an intensively used agricultural landscape

Meister

Höfer

Ursenbacher

et al. 2010

Biological Journal of the Linnean Society

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Both the conversion of natural habitats to farmland and efforts at increasing the yield of existing crops contribute to a decline in biodiversity. As a consequence of land conversion, specialised species are restricted to remnants of original habitat patches, which are frequently isolated. This may lead to a genetic differentiation of the subpopulations. We used seven microsatellite markers to examine the genetic population structure of the grass snake, Natrix natrix (Linnaeus, 1758), sampled in remnants of pristine habitat embedded in an intensively used agricultural landscape in north-western Switzerland. The study area, a former wetland, has been drained and gradually converted into an agricultural plain in the last century, reducing the pristine habitat to approximately 1% of the entire area. The grass snake feeds almost entirely on amphibians, and is therefore associated with wetlands. In Central Europe, the species shows severe decline, most probably as a result of wetland drainage and decrease of amphibian populations. We found no genetically distinct grass snake populations in the study area covering 90 km 2 . This implies that there is an exchange of individuals between small remnants of original habitat. Thus, gene flow may prevent any genetic differentiation of subpopulations distributed over a relatively large area. Our results show that a specialized snake species can persist in an intensively used agricultural landscape, provided that suitable habitat patches are interconnected.

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“…Genetische merkers voor individuele herkenning (microsatellieten) zijn beschikbaar in de literatuur (Meister et al, 2009) en op basis daarvan gemakkelijk operationeel te maken voor het beoogde onderzoek. Wanneer de nulmeting een ruimtelijke populatiestructuur laat zien die gerelateerd lijkt aan de (barrièrewerking van) infrastructuur, is na enkele jaren een effectmeting zinvol.…”

Section: Advies Populatie-genetisch Onderzoek Ringslangunclassified

Populatie-genetische nulmeting heikikker in de Vechtstreek (Noord-Holland) : en advies voor een studie naar de effecten van aangelegde faunapassages voor heikikker en ringslang

Grift¹,

Ottburg²,

Lammertsma³

et al. 2021

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Novel microsatellite loci in the grass snake (Natrix natrix) and cross‐amplification in the dice snake (Natrix tessellata)

Cited by 8 publications

References 11 publications

Integrative taxonomy provides evidence for the species status of the Ibero-Maghrebian grass snakeNatrix astreptophora

Integrative taxonomy provides evidence for the species status of the Ibero-Maghrebian grass snakeNatrix astreptophora

Spatial genetic analysis of the grass snake, Natrix natrix (Squamata: Colubridae), in an intensively used agricultural landscape

Populatie-genetische nulmeting heikikker in de Vechtstreek (Noord-Holland) : en advies voor een studie naar de effecten van aangelegde faunapassages voor heikikker en ringslang

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