Novel microsatellite markers for the endangered neotropical fish <i>Brycon orbignyanus</i> and cross-amplification in related species

Souza, Felipe Pinheiro de; Urrea-Rojas, Angela Maria; Ruas, Claudete de Fátima; Povh, Jayme Aparecido; Ribeiro, Ricardo Pereira; Ruas, Eduardo Augusto; Giacomin, Renata Mussoi; Góes, Bruna Delgado; Castro, Pedro Luiz de; Lopera-Barrero, Nelson Maurício

doi:10.1080/1828051x.2018.1436008

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…Thirty microsatellite primers were used for the cross-amplification tests, 13 of which were for the genus Brycon: bh5, bh6, bh8, bh13, bh16 (B. hilarii) (sAnches & GALeTi Jr, 2006); bc48-10 (B. cephalus) (bArroso, 2003); bom5, bom13 (B. opalinus) (bArroso et al, 2003) and borg9, borg13, borg56, borg59, borg65 (B. orbignyanus) (souZA et al, 2018). in addition, the following primers were tested: five primers for Piaractus mesopotamicus (Pme2, Pme4, Pme5, Pme20, Pme28) (cALcAGnoTTo et al 2001), five primers for Colossoma macropomum (cmA8, cmA11, cmc8, cmD1, cme3) (sAnTos et al, 2009), and seven primers for the genus Prochilodus, of which six were for P. argenteus (Par12, Par14, Par15, Par21, Par43, and Par80) (bArbosA et al, 2006;bArbosA et al, 2008) and one was for P. lineatus (Pli30) (YAZbeck & kALAPoThAkis, 2007).…”

Section: Methodsmentioning

confidence: 99%

Transferability of heterologous microsatellite primers in Brycon gouldingi

et al. 2018

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Brycon gouldingi is a species of neotropical fish of socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this species are still limited, making it difficult to evaluate the population structure and genetic diversity in natural and captive stocks. Here, we aimed to evaluate the transferability of heterologous microsatellite primers in B. gouldingi. A total of 30 primers for eight species were evaluated: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus, and Colossoma macropomum. The primers that showed the best amplification patterns were applied to 20 specimens of B. gouldingi, and their genetic parameters were assessed. Among the 30 primers, seven showed satisfactory transferability, six of which belonged to the genus Brycon: Bh13 (B. hilarii), BoM5, BoM13 (B. opalinus), Borg9, Borg13, and Borg59 (B. orbignyanus), and one belonged to P. argenteus (Par80). The primers for the other species tested showed non-specificity or monomorphism; and were therefore, excluded from the analyses. The number of alleles ranged between two (Borg13 and Borg59) and three (Bh13, BoM5, BoM13, Borg9 and Par80), with sizes varying between 103 bp (BoM5) and 430 bp (Borg9). Four primers showed evidence of null alleles (BoM13, Borg9, Borg13, and Par80), which could probably be attributed to the respective Hardy-Weinberg deviation. Thus, seven primers were validated for cross-amplification in B. gouldingi, which may be used in future studies involving this species.

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Section: Methodsmentioning

confidence: 99%

Transferability of heterologous microsatellite primers in Brycon gouldingi

et al. 2018

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“…Thirty-five primers were tested, of which seventeen belonged to Brycon: two were described by Barroso et al (2005) for B. opalinus (BoM5 and BoM13), five were described by Sanches and Galetti (2006) for B. hilarii (Bh5, Bh6, Bh8, Bh13, Bh16), one was described by Matsumoto and Hilsdorf (2009) for B. insignis (Bc48-10), three were described by Souza et al (2018a) for B. orbignyanus (Borg13, Borg25, and Borg59), and six were described by Araújo (2012) for B. amazonicus (Bag22, Bag25, Bag27, Bag31, Bam6, and Bam11). Additionally, we tested primers of related species: five described by Barbosa et al (2006) (Par12, Par14, Par15, Par21, and Par43) and two described by Barbosa et al (2008) (Par80, and Par82) for Prochilodus argenteus, one described by Yazbeck and Kalapothakis (2007) for Prochilodus lineatus (Pli30), five described by Calcagnotto et al (2001) for Piaractus mesopotamicus (Pme2, Pme4, Pme5, Pme20, and Pme28) and five described by Santos et al (2009) for Colossoma macropomum (Cm1A8, Cm1A11, Cm1C8, Cm1D1, and Cm1E3).…”

Section: Methodsmentioning

confidence: 99%

Transferability of heterologous primers in Brycon falcatus

et al. 2020

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The genusBryconcomprises fish species of significant socioeconomic and biological importance in Brazil. Despite that, the genetic knowledge about these species is scarce, especially regardingBrycon falcatus. Thus, the objective of this study was to evaluate the transferability of heterologous microsatellite primers inB. falcatus for the first time. Heterologous primers obtained from B. opalinus, B. hilarii, B. insignis, B. orbignyanus, B. amazonicus, Prochilodus argenteus, Prochilodus lineatus, Piaractus mesopotamicus, and Colossoma macropomum were evaluated. The primers that showed the best amplification patterns were applied to a sample of 22 individuals and the genetic parameters were calculated. Nine primers displayed satisfactory cross-amplification withB. falcatus: BoM5 (Brycon opalinus); Bh8, Bh13 and Bh16 (B. hilarii); Borg59 (B. orbignyanus); Bag22 (B. amazonicus); Par12 and Par80 (P. argenteus), and Cm1A8 (C. macropomum). The genetic parameters (number of alleles, effective alleles, allele richness, and expected and observed heterozygosity) and the polymorphic information content (PIC) confirmed the viability of these primers for population genetics analyses. Our study demonstrates the potential of transferability of microsatellite markers from related species and even different genera to B. falcatus, providing usefull tools for future population genetic studies in this species.

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“…For the cross-amplification tests, 32 microsatellite primers were used in five Leiarius marmoratus specimens, of which six primers belonged to the species Pseudoplatystoma punctifer: PPU1, PPU2, PPU4, PPU10, PPU13, PPU15 (Saulo-Machado et al, 2011); four primers described for Colossoma macropomum: Cm1A8, Cm1A11, Cm1D1, and Cm1E3 (Santos, Hrbek, & Farias, 2009); three described for Brycon hilarii: Bh6, Bh8, and Bh16 (Sanches & Galetti, 2006); four for Brachyplatystoma rousseauxii: BR38, BR47, BR51, and BR61 (Batista, 2010); three described for Brycon orbignyanus: Borg9, Borg13, and Borg4 (Souza et al, 2018b); four for Piaractus mesopotamicus: PME2, PME4, PME21 and PME32 (Calcagnotto, Russello, & DeSalle, 2001); two for Prochilodus argenteus: Par15 and Par21 (Barbosa, Correa, Galzerani, Galetti, & Hatanaka, 2006); two for Brycon opalinus: BoM5 and BoM13 (Barroso et al, 2003); and four primers described for Pseudoplatystoma corruscans: Pcor1, Pcor5, Pcor8, and Pcor10 (Revaldaves, Pereira, Foresti, & Oliveira, 2005).…”

Section: Methodsmentioning

confidence: 99%

Transferability of heterologous microsatellite primers in Leiarius marmoratus

Pandolfi

Yamachita

Souza

et al. 2020

Semina: Ciênc. Agrár.

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The Amazonian Jundiá (Leiarius marmoratus) (Siluliformes: Pimelodidae) is a species of catfish with social and economic importance in some South American countries such as Brazil and Colombia. Genetic evaluation of this species is limited due to the lack of specific molecular markers, hindering studies on genetic diversity and structure in animals under captive conditions or in natural populations. The objective of the present study was to evaluate the transferability of heterologous microsatellite markers in Leiarius marmoratus. Thirty-two heterologous primers were tested in L. marmoratus. The primers that presented the best standards were applied to 20 specimens, and the number of alleles (Na), number of effective alleles (Ne), gene diversity per Locus (GdL) and percentage of amplification failure (Md) were calculated. Eleven primers demonstrated satisfactory transferability patterns, all from the fish of the Pimelodidae family, of which, seven were monomorphic and four polymorphic. The eleven markers presented low percentage of Md (mean was 5.9% samples per locus). Na varied from one to two alleles per locus, revealing low polymorphism in the evaluated samples. The mean Ne and GdL numbers were 1.77 and 0.32, respectively. The transferability of the heterologous microsatellite loci in L. marmoratus was shown to be possible. However, further tests are needed to apply these markers in population genetic studies.

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Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species

Cited by 9 publications

References 20 publications

Transferability of heterologous microsatellite primers in Brycon gouldingi

Transferability of heterologous microsatellite primers in Brycon gouldingi

Transferability of heterologous primers in Brycon falcatus

Transferability of heterologous microsatellite primers in Leiarius marmoratus

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