Novel microscopy in arbitrary step digital holography

Abstract

In this work, a novel approach for numerical wave-front reconstruction in arbitrary phase step digital holography is presented. We present a simple and effective approach for digital holography microscopy that can be used for the 3D reconstruction of micro-structure images. The experimental results demonstrate that only two digital holograms and a simple estimation are required for the twin-image suppression and numerical reconstruction. The advantages of this approach are its simplicity, in that only one estimate equation need be applied, and its effectiveness, in that the exact phase profile of a micro lens array is presented, without blurring due to numerical reconstruction or aberration caused by the quadratic phase micro objective lens.