Novel mitochondrial proteins and decreased intrinsic activity of cytochrome-C Oxidase

Alleyne, Trevor; Adogwa, Andrew; Lalla, A.; Joseph, Jerome; John, Roger

doi:10.1007/bf02815234

Cited by 8 publications

(4 citation statements)

References 16 publications

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“…Low copper in the diet during the pre-and perinatal period leads to oxidative degeneration of the CNS tissue with a secondary Wallerian degeneration of the myelin. 40,41 Loss of myelin in the dorsolateral spinal cord tracts, or, to a lesser extent, the formation of cavitations in the white matter of the brain are the histopathologic hallmarks of this disease.…”

Section: 31mentioning

confidence: 99%

Magnetic Resonance Imaging Findings in Metabolic and Toxic Disorders of 3 Small Ruminants

Schenk

Ganter

Seehusen

et al. 2007

Veterinary Internal Medicne

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A 2-month-old female Boer Goat with microphthalmia since birth was referred to the Clinic for Pigs and Small Ruminants and Ambulatory Service of the University of Veterinary Medicine, Hannover, because of a 3-day period of inappetence. Physical examination revealed no abnormalities except for bilateral microphthalmia. During neurologic examination, apathy and pleurothotonus to the right in combination with a circling gait in the same direction were observed. Postural reactions were reduced on the left side and the goat was blind. The neuroanatomic localization of the disease was forebrain lesion on the right side.The following differential diagnoses were considered: anomaly (eg, hydrocephalus), an inflammatory disease (including parasitic infection), or a metabolic or toxic lesion. In this context and in the following reports the term metabolic disturbance refers to endogenous metabolic abnormalities and to nutritional deficiencies. Although the clinical signs were asymmetric, a metabolic or toxic lesion was included in the differential diagnosis list as in some diffuse intracranial lesions one side can be affected more severely than the other. Radiographs of the skull revealed no abnormalities. The CBC and serum biochemistry results were normal. The owners wanted to keep the goat as a pet and further investigations were carried out.The sum of thiamine monophosphate (TMP), thiamine diphosphate (TDP), and vitamin B1 concentrations in the blood as an indicator for thiamine deficiency was 0.123 mg/L (reference range, 0.044-0.08 mg/L). Serum antibodies against caprine encephalitis arthritis virus (CAE-V) and border disease virus (BD-V) could not be detected. The goat was anesthetized with xylazine a at a dosage of 0.05 mg/kg IM and ketamine b at a dosage of 4 mg/kg IV. Cerebrospinal fluid (CSF) was collected from the cisterna magna. The CSF cell count and protein concentrations were normal. Anesthesia was maintained with isoflurane c and oxygen via a Draeger respirator d and magnetic resonance imaging (MRI) was performed after 6 days of symptomatic treatment including antibiotics, IV fluids, PO administration of rumen fluid from a healthy donor, and thiamine supplements (Thiasel, e 100 mg/kg IV). Treatment did not alleviate the neurologic signs.The MRI of all 3 patients described in this report was performed with a 1.0 Tesla scanner.f The anesthetized animal was placed in sternal recumbency with the head positioned in a head coil.The imaging protocol included a T1-weighted spin echo (SE) (repetition time [TR] in milliseconds [ms] of 330.0 ms and echo time [TE] of 12.0 ms) sequence, a T2-weighted turbo spin echo (TSE; TR: 3,458.0 ms; TE: 96.0 ms), and a fluid attenuated inversion recovery (FLAIR) sequence (TR: 9,000.0 ms; TE: 105.0 ms). These 3 sequences were performed in sagittal, transverse, and dorsal planes. Furthermore, the T1-weighted SE sequence was performed a second time at the end of the other scans after IV administration of gadoliniumdimegluminie (Magnevist, g 0.2 mmol/kg). Compared to unaffected brain t...

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Section: 31mentioning

confidence: 99%

Magnetic Resonance Imaging Findings in Metabolic and Toxic Disorders of 3 Small Ruminants

Schenk

Ganter

Seehusen

et al. 2007

Veterinary Internal Medicne

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“…Copper deficiency is known to cause an ataxic myelopathy in ruminants called swayback. 12 Decreased activity of a copper metalloenzyme has been shown in swayback 13 and Menkes disease. 7 In humans, the well recognized disorders of copper deficiency are Menkes disease 8 and the occipital horn syndrome.…”

mentioning

confidence: 99%

Copper deficiency myelopathy produces a clinical picture like subacute combined degeneration

Kumar¹,

Gross²,

Ahlskog³

2004

Neurology

289

221

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“…Normally the liver tissue and similarly the kidney tissue from three to five animals in a particular group would be combined prior to commencing the isolation of mitochondria. Mitochondria were extracted from the liver and kidney tissue by acid precipitation as described previously [28,29] and stored at -20 °C until needed; kinetic studies were normally performed within 48 h of storage.…”

Section: Methodsmentioning

confidence: 99%

“…The rate of oxygen consumption of the mitochondria isolated from diabetic and control rats was determined by polarograhic assay as described elsewhere [24,28]. The final mitochondrial concentration was 2 mg/mL and the concentration of cytochrome c (the substrate) ranged from 6.8 to 680 μM; a small volume of buffer was added as necessary to maintain a fixed reaction volume of 3.0 mL.…”

Section: Polarographic Assaymentioning

confidence: 99%

The Impact of Diabetes Mellitus on Oxygen Utilization by Complex IV: Preliminary Insights

et al. 2017

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Background: The protein complexes of the electron transport chain have been linked to the pathogenesis of diabetes mellitus (DM), but the interplay between DM and cytochrome c oxidase (complex IV) is not well understood. In this study, using a rat DM model, we evaluated the effect of DM on liver and kidney mitochondria, looking specifically at the characteristics of oxygen consumption by complex IV; we also studied the effects of DM on the protein composition of liver and kidney mitochondria.

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Novel mitochondrial proteins and decreased intrinsic activity of cytochrome-C Oxidase

Cited by 8 publications

References 16 publications

Magnetic Resonance Imaging Findings in Metabolic and Toxic Disorders of 3 Small Ruminants

Magnetic Resonance Imaging Findings in Metabolic and Toxic Disorders of 3 Small Ruminants

Copper deficiency myelopathy produces a clinical picture like subacute combined degeneration

The Impact of Diabetes Mellitus on Oxygen Utilization by Complex IV: Preliminary Insights

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