Novel Molecular Variants of Allele I of the
            <i>Escherichia coli</i>
            P Fimbrial Adhesin Gene
            <i>papG</i>

Johnson, James R.; Stell, Adam L.; Kaster, Nicholas; Fasching, Claudine E.; O'Bryan, Timothy T.

doi:10.1128/iai.70.5.2739a-2739a.2002

Cited by 4 publications

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“…The pap gene cluster consists of 11 genes encoding; the main component of the fimbria rod (papA), papEF, which encodes adaptor subunits, and a terminal adhesin gene papG. The papG subunit occurs in 4 molecular variants, including classes I-IV, each encoded by a distinct allele of the adhesin gene papG [7,8]. Each of these 4 papG molecular variants have distinct receptor binding specificities, and hence understood to confer differences in host range specificities [9][10][11] and /or capacity to cause specific UTI clinical syndromes [9,12,13], albeit some contradicting findings in other studies [14].…”

Section: Introductionmentioning

confidence: 99%

Distribution of papG alleles among uropathogenic Escherichia coli from reproductive age women

Kudinha

Kong

2022

J Biomed Sci

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Background Extraintestinal Escherichia coli (E. coli) causing urinary tract infections (UTIs), and often referred to as uropathogenic E. coli (UPEC), are a major contributor to the morbidity of UTIs and associated healthcare costs. UPEC possess several virulence factors (VFs) for infecting and injuring the host. We studied the papG allele distribution, and its association with other VF genes and phylogenetic groups, amongst 836 UPEC and fecal isolates from reproductive age women. Results The papGII gene was highly prevalent amongst pyelonephritis isolates (68%), whilst the majority, albeit smaller proportion, of cystitis isolates (31%) harboured the papGIII gene. Among the pyelonephritis and cystitis isolates, papG positive isolates on average had higher VF gene scores, and were more likely to belong to phylogenetic group B2, than their negative counterparts. This was mostly due to the contribution of papGII isolates, which on average contained more VF genes than their papGIII counterparts, irrespective of the uro-clinical syndrome. However, the papGII isolates from the pyelonephritis cohort had higher VF gene scores than the cystitis ones, suggesting presence of possible papGII clones with differing inferred virulence potential. Furthermore, papGII isolates were more likely to possess an intact pap gene operon than their papGIII counterparts. Also of note was the high proportion of isolates with the papGI allele which was not associated with other pap operon genes; and this finding has not been described before. Conclusions The association of the papGII gene with several VF genes compared to the papGIII gene, appears to explain the abundance of these genes in pyelonephritis and cystitis isolates, respectively.

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Section: Introductionmentioning

confidence: 99%

Distribution of papG alleles among uropathogenic Escherichia coli from reproductive age women

Kudinha

Kong

2022

J Biomed Sci

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“…Some diarrheagenic E.coli strains have the ability to tolerate exposure to pH 2.0. Such an acid shock mimics transit through the stomach and induces expression of sets of genes involved in survival and pathogenesis (Braun and Braun,2002).Clinically, two distinct types of pathogenic E. coli are recognized ;One group commonly called extraintestinal pathogenic E. coli (ExPEC) includes those E. coli associated with newborn meningitis (NBM) or sepsis and urinary tract infections (UTIs) (Johnson et al, 2001).Several virulence factors (VFs) enhance the capacity of E. coli to cause systemic infections; unlike most commensal E.coli strains, extraintestinal isolates possess genes encoding putative VFs of extraintestinal pathogenic E.coli(ExPEC),which include various combinations of adhesins (P and S fimbriae),toxins(e.g., hemolysin and cytonecrotizing factor),polysaccharide coatings including ( capsules and lipopolysaccharides), siderophores or iron acquisition systems (e.g.,aerobactin and yersiniabactin), serum resistance mechanisms, and invasins ,These VFs help the organisms to colonize host surfaces, avoid and/or subvert host defense mechanisms, injure and/or invade host cells and tissues, and incite a noxious inflammatory response, thereby leading to clinical disease (Bingen-Bidois et al .,2002 (Donnenberg, 2002;Kaper et al,2004). E.coli serotypes defined by serum antibodies of the patient or animals that identify the specific type of antigen presented by bacteria.…”

Section: Introductionmentioning

confidence: 99%

Genotyping of Escherichia Coli Isolated From Clinical and Hospitals Environment

2019

University of Thi-Qar Journal of Science

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Pathogenic Escherichia coli (E. coli) strains are known to cause intestinal and extraintestinal infections in human. A wide variety of infectious diseases could be caused by E. coli strains, including urinary tract infection, septicemia, newborn meningitis, central nervous system and respiratory system infections.Several bacterial agents can cause diarrheal and urinary tract infections, among these E.coli strains are detected as an important cause of morbidity and mortality of diarrhoea and UTI throughout the world.A total of 318 samples were collected during the period from September 2012 to march 2013 .All specimens were screened for the presence of E. coli by cultured on MacConkey agar and Eosin-methylene blue agar then identified by biochemical tests and confirmed by API 20E system which revealed that:90/318(28.33%) gave positive growth for E.coli)15 (16.63%) environmental isolates and 75(83.33%) clinical isolates(as fallowing:1/42(2.38%)was obtained from burns,7/25(28%)from High vaginal swabs ,11/41 (26.82%) from Wounds infections,18/33(54.54%)from Diarrheal infections and38/67(56.71 %)from Urinary tract infections, while 15/110 (13.63%) from environmental isolates, The isolated were subjected to molecular identification as E.coli by used the intergenic spacer region primer(ITS). The confirmed isolates were examined to detect the phylogenetic group based on triplex PCR by using a combination of two genes (chuA and yjaA) and an anonymous DNA fragment.Universal primers (ITS) indicated that all isolates 90(100%) gave positive results.The phylogenetic analysis revealed that 90 E.coli isolates belonged to three phylogenetic groups A &D 36(40%)for each, while B2 18 (20%) and no one isolates belong to B1 phylogenetic types .Out of the 16 of antibiotics used, 87(92.22%) isolates were multidrug resistance.All isolates have been found resistant to at least one β-lactam Antibiotics.

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“…Variações mais abrangentes, que podem ser detectadas pelo uso de iniciadores ou sondas, ocorrem entre os vários alelos conhecidos de papG (adesina da fímbria P) (Johanson et al, 1993;Johnson et al, 2001b;Karkkainen et al, 1998). As variantes peptídicas correspondem a ligações preferenciais diferentes ao componente Gal( 1-4)Gal dos glicopeptídeos (Stapleton et al, 1998;Stroud et al, 1998)…”

Section: E Coliunclassified

Metodos biologicos e moleculares no estudo de fatores de virulencia em amostras de Eschericia coli isoladas por hemocultura de pacientes com septicemia

Ananias¹,

Yano²

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Conheço as tuas obras: não és nem frio nem quente. Oxalá fosses frio ou quente! Mas, como és morno, nem frio e nem quente, vou vomitar-te.". Apocalipse 3, 15-16. v Dedico este trabalho aos meus pais, pelo exemplo que representam, pelo incentivo que me deram e pelo amor compartilhado durante toda minha vida. vi AGRADECIMENTOS A Deus, por estar sempre presente em minha vida e pela sua imensa bondade, permitindo-me a conquista de tantas coisas.Aos meus pais e irmãos, que sempre me apoiaram e principalmente pelo amor que me deram.Agradeço enormemente ao meu orientador, Prof. Dr. Tomomasa Yano, pela excelente orientação, por toda confiança e apoio e pela grande pessoa que é. Ao senhor, toda a minha admiração.

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Novel Molecular Variants of Allele I of the Escherichia coli P Fimbrial Adhesin Gene papG

Cited by 4 publications

References 0 publications

Distribution of papG alleles among uropathogenic Escherichia coli from reproductive age women

Distribution of papG alleles among uropathogenic Escherichia coli from reproductive age women

Genotyping of Escherichia Coli Isolated From Clinical and Hospitals Environment

Metodos biologicos e moleculares no estudo de fatores de virulencia em amostras de Eschericia coli isoladas por hemocultura de pacientes com septicemia

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