Novel Multilocus Sequence Typing Scheme Reveals High Genetic Diversity of Human Pathogenic Members of theFusarium incarnatum-F.equisetiandF. chlamydosporumSpecies Complexes within the United States

Abstract

Species limits within the clinically important Fusarium incarnatum-F. equiseti and F. chlamydosporum species complexes (FIESC and FCSC, respectively) were investigated using multilocus DNA sequence data. Maximumparsimony and maximum-likelihood analyses of aligned DNA sequences from four loci resolved 28 species within the FIESC, within which the species were evenly divided among two clades designated Incarnatum and Equiseti, and four species within the FCSC. Sequence data from a fifth locus, ␤-tubulin, was excluded from the study due to the presence of highly divergent paralogs or xenologs. The multilocus haplotype nomenclature adopted in a previous study

Phylogenetic analyses and toxigenic profiles of Fusarium equiseti and Fusarium acuminatum isolated from cereals from Southern Europe

“…Indeed, the present results, and those of a recent study on the clinically important F incarnatum-F equiseti species complex involving the use of multilocus DNA sequence data (O'Donnell et al, 2009), show the strong variability of F equiseti and F scirpi. O'Donnell et al (2009) concluded that the F incarnatum-F. equiseti complex contained 28 phylogenetically distinct species in which both F equiseti and F. scirpi were represented in two distinct clusters. These F equiseti and F scirpi strains were also included in the present phylogenetic study, and they clustered within F equiseti type I and F scirpi respectively.…”

“…Neither do such methods detect intraspecific variability. Fortunately, phylogenetic analyses that make use of DNA sequence data have made significant contributions to our understanding of the systematics of Fusarium, providing species boundaries that are essential for establishing inter-and intraspecific relationships with respect to toxin profiles (Jurado et al, 2006a;Kristensen et al, 2005;Mirete et al, 2004;O'Donnell et al, 2009). Additionally, these techniques have provided the basis necessary for developing rapid, specific and accurate diagnostic methods based on PCR.…”

“…This species is often detected in Norwegian cereals , but it is also common in Southern Europe (Logrieco et al, 2003), particularly in Spain (Jurado et al, 2006a;Marin, 2010;Soldevilla et al, 2005). Further, it belongs to the socalled Fusarium incamatum-F. equiseti species complex, a genetically highly diverse group (O'Donnell et al, 2009) the members of which are associated with human disease.…”

Challenges in Fusarium, a Trans-Kingdom Pathogen

“…The opportunists on humans group into seven species complexes: the Fusarium solani, F. oxysporum, F. incarnatum-equiseti, F. fujikuroi, F. clamydosporum, F. dimerum and F. sporotrichioides species complexes [4]. In particular members of the Fusarium solani and F. oxysporum species complexes have been implicated in human infections worldwide [8][9][10], but local, endemic plant pathogens prove to have virulence to humans as well (e.g., [4]). Many molecular siblings have no binomial designations yet.…”

First report of Fusarium lacertarum causing cladode rot in Nopalea cochenellifera in Brazil

“…These morphological characteristics tallied with those of Fusarium lacertarum (Leslie and Summerell 2006). The elongation factor-1α region was sequenced and compared with that of other Fusarium species from Fusarium-ID database, and subsequently in a maximum likelihood phylogenetic analysis including previously published sequences (O'Donnell et al 2009). Our isolate (URM7639, GenBank accession No.…”