Novel NDM-9 metallo-β-lactamase identified from a ST107 Klebsiella pneumoniae strain isolated in China

Wang, Xiaojuan; Li, Henan; Zhao, Chunjiang; Chen, Hongbin; Liu, Jingbo; Wang, Zhanwei; Qi, Wang; Zhang, Yawei; He, Wenqiang; Zhang, Feifei; Wang, Hui

doi:10.1016/j.ijantimicag.2014.04.010

Cited by 47 publications

(31 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most prevalent bla subtypes from the transconjugants were NDM-9 (7/12) and NDM-1 (3/12) ( Table 1). The NDM-9 subtype was first identified on a 180-kb IncH plasmid from a clinical strain of Klebsiella pneumoniae in China, and it conferred higher levels of resistance to carbapenem than NDM-1 (19). Our results suggest that the NDM-9 allele has been an important subtype resulting in the high-level carbapenem resistance among Enterobacteriaceae in China.…”

mentioning

confidence: 63%

High Incidence of Escherichia coli Strains Coharboring mcr-1 and bla _NDM from Chickens

Liu

Song

Zou

et al. 2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

This study investigated the characteristics of Escherichia coli isolates carrying mcr-1-bla NDM from a chicken farm in China. Of the 78 E. coli isolates, 21 clonally unrelated isolates carried mcr-1-bla NDM . Diverse IncI2 plasmids disseminated mcr-1, while the dissemination of bla NDM was mediated by diverse IncB/O plasmids. More striking was the colocalization of resistance genes mcr-1 and bla in an IncHI2/ST3 plasmid, which might pose a great challenge for public health.KEYWORDS high incidence, mcr-1, bla NDM , colocalization, Escherichia coli C arbapenems have been reliable and potent agents against Gram-negative bacteria. The rapid increase of carbapenem-resistant Enterobacteriaceae poses a great threat to public health and has prompted the reconsideration of colistin as a last-resort therapeutic option (1). Recently, a plasmid-borne colistin resistance gene (mcr-1) was identified in Escherichia coli and Klebsiella pneumoniae strains from animals and humans in China (2). This finding foreshadowed the inevitable dissemination of colistin resistance worldwide and was confirmed by the presence of mcr-1 in other countries (3-6).The mcr-1 gene is often associated with the extended-spectrum ␤-lactamase gene and has been found with bla CTX-M in the same plasmid (4,7,8). The mcr-1 gene has also emerged in carbapenem-resistant isolates (4, 9, 10), and the cotransfer of mcr-1 and carbapenem resistance genes is obviously of great clinical concern. Recently, mcr-1 was even found with bla NDM in the same IncX3-X4 hybrid plasmid in E. coli from pets in China (11). However, only a few isolates resistant to both carbapenem and colistin were characterized in those previous studies.Food-producing animals, especially chickens, serve as resistance gene "reservoirs," so it is crucial to identify the origins of multidrug-resistant plasmids in these animals. In this study, we investigated the genetically diverse E. coli isolates carrying mcr-1 and bla NDM from a chicken farm and characterized the plasmids harboring mcr-1 or bla NDM .Seventy-eight E. coli isolates were collected from diseased chickens in four separate barns on a large chicken farm (100,000 animals) in Shandong Province, China in October 2015. Feces from chickens that showed signs of diarrhea were randomly collected and streaked onto MacConkey agar. After incubating at 37°C for 20 h, one colony with typical E. coli morphology was selected from each chicken sample. Although detailed information on antibiotic usage was not available, ceftiofur and colistin were often used for prophylaxis and treatment of bacterial infections on this farm.Susceptibilities to 18 antimicrobials were determined for these 78 isolates by the agar dilution method using the recommended breakpoints (12, 13). The colistin breakpoint (Ն2 g/ml) was used according to recommendations by the European Committee for Antimicrobial Susceptibility Testing.

show abstract

mentioning

confidence: 63%

High Incidence of Escherichia coli Strains Coharboring mcr-1 and bla _NDM from Chickens

Liu

Song

Zou

et al. 2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…There are 10 variants of the NDM enzyme discovered to date (39)(40)(41), with little functional difference between them in terms of carbapenem hydrolysis, although differences in thermal stability and the hydrolysis of certain cephalosporins were detected (42).…”

Section: Enterobacteriaceaementioning

confidence: 99%

Carbapenem-Resistant Acinetobacter baumannii and Enterobacteriaceae in South and Southeast Asia

et al. 2017

View full text Add to dashboard Cite

SUMMARY Carbapenem-resistant Gram-negative bacteria, in particular the Acinetobacter baumannii-calcoaceticus complex and Enterobacteriaceae, are escalating global public health threats. We review the epidemiology and prevalence of these carbapenem-resistant Gram-negative bacteria among countries in South and Southeast Asia, where the rates of resistance are some of the highest in the world. These countries house more than a third of the world's population, and several are also major medical tourism destinations. There are significant data gaps, and the almost universal lack of comprehensive surveillance programs that include molecular epidemiologic testing has made it difficult to understand the origins and extent of the problem in depth. A complex combination of factors such as inappropriate prescription of antibiotics, overstretched health systems, and international travel (including the phenomenon of medical tourism) probably led to the rapid rise and spread of these bacteria in hospitals in South and Southeast Asia. In India, Pakistan, and Vietnam, carbapenem-resistant Enterobacteriaceae have also been found in the environment and community, likely as a consequence of poor environmental hygiene and sanitation. Considerable political will and effort, including from countries outside these regions, are vital in order to reduce the prevalence of such bacteria in South and Southeast Asia and prevent their global spread.

show abstract

“…NDM-9 differing by a single amino acid substitution (E152K) from NDM-1 was recently identified in K. pneumoniae ST107 strain from China [93]. …”

Section: Introductionmentioning

confidence: 99%

Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health

Khan

Maryam

Zarrilli³

2017

BMC Microbiol

427

295

View full text Add to dashboard Cite

BackgroundThe emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria.Main bodyIn this review, we have discussed bla NDM-1variants, its genetic analysis including type of specific mutation, origin of country and spread among several type of bacterial species. Wide members of enterobacteriaceae, most commonly Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, and gram-negative non-fermenters Pseudomonas spp. and Acinetobacter baumannii were found to carry these markers. Moreover, at least seventeen variants of bla NDM-type gene differing into one or two residues of amino acids at distinct positions have been reported so far among different species of bacteria from different countries. The genetic and structural studies of these variants are important to understand the mechanism of antibiotic hydrolysis as well as to design new molecules with inhibitory activity against antibiotics.ConclusionThis review provides a comprehensive view of structural differences among NDM-1 variants, which are a driving force behind their spread across the globe.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-1012-8) contains supplementary material, which is available to authorized users.

show abstract

Novel NDM-9 metallo-β-lactamase identified from a ST107 Klebsiella pneumoniae strain isolated in China

Cited by 47 publications

References 5 publications

High Incidence of Escherichia coli Strains Coharboring mcr-1 and bla _NDM from Chickens

High Incidence of Escherichia coli Strains Coharboring mcr-1 and bla _NDM from Chickens

Carbapenem-Resistant Acinetobacter baumannii and Enterobacteriaceae in South and Southeast Asia

Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health

Contact Info

Product

Resources

About

Novel NDM-9 metallo-β-lactamase identified from a ST107 Klebsiella pneumoniae strain isolated in China

Cited by 47 publications

References 5 publications

High Incidence of Escherichia coli Strains Coharboring mcr-1 and bla NDM from Chickens

High Incidence of Escherichia coli Strains Coharboring mcr-1 and bla NDM from Chickens

Carbapenem-Resistant Acinetobacter baumannii and Enterobacteriaceae in South and Southeast Asia

Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health

Contact Info

Product

Resources

About

High Incidence of Escherichia coli Strains Coharboring mcr-1 and bla _NDM from Chickens

High Incidence of Escherichia coli Strains Coharboring mcr-1 and bla _NDM from Chickens