Novel neural adhesion molecules in the Contactin/F3 subgroup of the immunoglobulin superfamily: isolation and characterization of cDNAs from rat brain

Ogawa, Jun; Kaneko, Hitoshi; Matsumoto, Takayuki; Nagata, Saburo; Hosoya, Hiroko; Watanabe, Kazutada

doi:10.1016/s0304-3940(96)13156-6

Cited by 58 publications

(45 citation statements)

References 19 publications

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“…The results revealed binding of APP to contactins 3 and 4. This fits with the homology relationships, as contactins 3 and 4 are the most closely related pair within the contactin family (Ogawa et al, 1996). APLP1 bound contactins 3 and 4, and also contactin 5.…”

Section: App Binds Specific Contactinssupporting

confidence: 84%

Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system

et al. 2008

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The amyloid precursor protein (APP) plays a central role in Alzheimer's disease, but its actions in normal development are not well understood. Here, a tagged APP ectodomain was used to identify extracellular binding partners in developing chick brain. Prominent binding sites were seen in the olfactory bulb and on retinal axons growing into the optic tectum. Co-precipitation from these tissues and tandem mass spectrometry led to the identification of two associated proteins: contactin 4 and NgCAM. In vitro binding studies revealed direct interactions among multiple members of the APP and contactin protein families. Levels of the APP processing fragment, CTF␣, were modulated by both contactin 4 and NgCAM. In the developing retinotectal system, APP, contactin 4 and NgCAM are expressed in the retina and tectum in suitable locations to interact. Functional assays revealed regulatory effects of both APP and contactin 4 on NgCAM-dependent growth of cultured retinal axons, demonstrating specific functional interactions among these proteins. These studies identify novel binding and functional interactions among proteins of the APP, contactin and L1CAM families, with general implications for mechanisms of APP action in neural development and disease.

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Section: App Binds Specific Contactinssupporting

confidence: 84%

Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system

et al. 2008

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“…The sections were stained with 1% 2,3,5-triphenyltetrazolium chloride for 30 minutes at 37°C and fixed in 4% paraformaldehyde. The area (unstained) of the infarction in the left cerebral hemisphere was then traced and measured using image analysis software (Image Pro-Plus 6.0), and the infarction volume per brain (mm 3 ) was calculated from the measured infarction area. 11…”

Section: Measurement Of Infarction Volumementioning

confidence: 99%

“…3,5 To investigate the role of NB-3 after ischemic injury, we examined NB-3 expression in the adult mouse brain. NB-3-deficient mice were generated by substituting a part of the NB-3 gene with the galactosidase (LacZ) gene.…”

Section: Expression and Distribution Of Nb-3 In The Adult Mouse Brainmentioning

confidence: 99%

“…NB-3, also termed contactin-6, is a member of the contactin/F3 subgroup in the immunoglobulin superfamily. 3 The extracellular part of NB-3 consists of 6 immunoglobulin-like domains and 4 fibronectin type III repeats and provides the structural basis for the homophilic nature of NB-3 as well as its numerous heterophilic interactions. 4 A greater understanding of the functional roles of NB-3 is important to improve the targeting of recognition molecules that exert adverse effects on neuroregeneration after an acute lesion.…”

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confidence: 99%

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Loss of NB-3 Aggravates Cerebral Ischemia by Impairing Neuron Survival and Neurite Growth

Huang

Sun

Zhao

et al. 2011

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Background and Purpose-NB-3 is a member of the F3/contactin family of neural recognition molecules, which are crucial for cell morphogenesis and motility. NB-3 is expressed in neurons and plays an important role in axonal extension and neuronal survival. However, the role of NB-3 in cerebral ischemic injury remains unknown. Methods-Adult male wild-type and NB-3 knockout mice were subjected to ischemic injury by unilateral middle cerebral carotid artery occlusion for 3 hours, 6 hours, and 12 hours. Ischemic infarction volumes were then determined by 2, 3, 5-triphenyltetrazolium chloride staining. Neurological dysfunction analysis was also performed. Primary culture of neuronal cells from wild-type and knockout animals was also used for analysis of neuronal survival and neurite outgrowth. Results-NB-3 expression in the ischemic hemisphere was decreased after transient middle cerebral artery occlusion (MCAO). NB-3-knockout mice developed a 2.6-fold larger infarct volume and exhibited increased neurological deficit scores after transient middle cerebral artery occlusion compared with control mice. Substrate with NB-3 promoted neuronal survival and neurite outgrowth in vitro, whereas neurite outgrowth and neuronal survival were significantly reduced in NB-3-deficient neurons. In addition, NB-3 deficiency renders neurons more susceptible to oxygen-glucose deprivation-induced damage and NB-3 as substrate could partially through homophilic mechanisms. Conclusions-These data demonstrate that NB-3 deficiency may aggravate brain damage after middle cerebral artery occlusion by impairing neuronal survival and neurite growth.

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“…The vertebrate contactin subfamily of GPI-anchored Ig-CAMs comprises TAG-1/axonin-1, and several other related proteins, BIG-1, BIG-2, NB-2/FAR-2 and NB-3 (Furley et al, 1990;Yoshihara et al, 1995;Ogawa et al, 1996;Plagge et al, 2001). A Blast search of the Drosophila genome using the human contactin sequence identified a single Drosophila gene, CG1084 (FlyBase), with high sequence homology to contactin family members, which was named cont.…”

Section: Structural Comparison Between Cont and Its Mammalian Homologsmentioning

confidence: 99%

Drosophila contactin, a homolog of vertebrate contactin, is required for septate junction organization and paracellular barrier function

et al. 2004

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Novel neural adhesion molecules in the Contactin/F3 subgroup of the immunoglobulin superfamily: isolation and characterization of cDNAs from rat brain

Cited by 58 publications

References 19 publications

Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system

Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system

Loss of NB-3 Aggravates Cerebral Ischemia by Impairing Neuron Survival and Neurite Growth

Drosophila contactin, a homolog of vertebrate contactin, is required for septate junction organization and paracellular barrier function

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